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22874 Methadone, Urine

Methadone, Urine
Test Code: METD
Synonyms/Keywords
​​Dolophine
Useful For
Detecting exposure to methadone to monitor compliance in taking prescribed medicaitons, compliance in rehabilitation programs, or to detect abuse.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Urine​ Sage urine collection container​ Sterile plastic container with no preservatives​ 5 mL​ 2 mL​ 1 mL​
Specimen Stability Information
Specimen Type Temperature Time
Urine​ ​ Ambient​ 72 hours​
Refrigerate (Preferred)​ 30 days​
Frozen​ 30 days​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Friday​
If no confirmatory work is required: 1 day​
 
If confirmatory work is required: 4 days
 Beckman AU680 Clinical Chemistry System with Emit Immunoassay and (if needed) Liquid Chromatography/ Tandem Mass Spectrometry (LCMS/MS) using Multiple Reaction Monitoring
Test Information
This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the US Food and Drug Administration.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.
 
Methadone (Dolophine) was first synthesized as a morphine substitute.  Since it causes marked sedation, it has been used since 1965 as a narcotic maintenance treatment of former heroin addicts.  Methadone is metabolized to EDDP (2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidone).  When a person uses methadone, the main products that appear in urine are methadone and EDDP.   The test will detect the presence of parent methadone and EDDP (methadone metabolite).
 
This test is a screen for Methadone by immunoassay; if the screen is positive, this test automatically includes LCMS/MS confirmation for Methadone and EDDP. Quantitation/confirmation charged separately if needed.  It is intended for use only in patients where an initial drug screening test has not already been performed (i.e. Point-of-Care drug screening cup, dipstick, etc.).
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative.  Positives are reported with a quantitative LCMS/MS result.​
Interpretation

​Current Cutoff Levels for Toxicology

Methadone and EDDP are both excreted in the urine of a person taking methadone.  Methadone, however, is highly sensitive to urine pH, whereas EDDP is not.  Therefore, it is preferable to monitor EDDP in urine for assessing compliance in drug rehabilitation therapy. 

Methadone and EDDP can be detected up to 6-7 days after last use.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
80307​ ​ 1​ Single Drug Class​ ​
80358 ​ 1​ Methadone Confirmation ​If needed
​G0480 ​ ​1 Methadone Confirmation ​For Medicare/Medicaid, If needed
Classification
This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the US Food and Drug Administration.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.
Synonyms/Keywords
​​Dolophine
Ordering Applications
Ordering Application Description
​Cerner Methadone, Urine
​COM ​Methadone, Urine
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Urine​ Sage urine collection container​ Sterile plastic container with no preservatives​ 5 mL​ 2 mL​ 1 mL​
Specimen Stability Information
Specimen Type Temperature Time
Urine​ ​ Ambient​ 72 hours​
Refrigerate (Preferred)​ 30 days​
Frozen​ 30 days​
Useful For
Detecting exposure to methadone to monitor compliance in taking prescribed medicaitons, compliance in rehabilitation programs, or to detect abuse.
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative.  Positives are reported with a quantitative LCMS/MS result.​
Interpretation

​Current Cutoff Levels for Toxicology

Methadone and EDDP are both excreted in the urine of a person taking methadone.  Methadone, however, is highly sensitive to urine pH, whereas EDDP is not.  Therefore, it is preferable to monitor EDDP in urine for assessing compliance in drug rehabilitation therapy. 

Methadone and EDDP can be detected up to 6-7 days after last use.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Friday​
If no confirmatory work is required: 1 day​
 
If confirmatory work is required: 4 days
 Beckman AU680 Clinical Chemistry System with Emit Immunoassay and (if needed) Liquid Chromatography/ Tandem Mass Spectrometry (LCMS/MS) using Multiple Reaction Monitoring
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
80307​ ​ 1​ Single Drug Class​ ​
80358 ​ 1​ Methadone Confirmation ​If needed
​G0480 ​ ​1 Methadone Confirmation ​For Medicare/Medicaid, If needed
Classification
This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the US Food and Drug Administration.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.
For most current information refer to the Marshfield Laboratory online reference manual.