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26083 Epstein-Barr Virus DNA Detection & Quantification, Plasma (EBVQU)

Epstein-Barr Virus DNA Detection & Quantification, Plasma (EBVQU)
Test Code: EBVQUSO
Synonyms/Keywords
EBV, Infectious Mononucleosis, Viral Load, Post Transplant Lymphoproliferative Disorder (PTLD)
Useful For

Diagnosis of EBV-associated infectious mononucleosis in individuals with equivocal or discordant Epstein-Barr virus (EBV) serologic marker test results.

Diagnosis of posttransplant lymphoproliferative disorders (PTLD), especially in EBV-seronegative organ transplant recipients receiving antilymphocyte globulin for induction immunosuppression and OKT-3 treatment for early organ rejection.

Monitoring progression of EBV-associated PTLD in organ transplant recipients.

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Plasma ​EDTA Lavender Top Tube (LTT) ​1.5 mL ​0.8 mL
Collection Processing Instructions
Centrifuge blood collection tube per collection tube manufacturer's instructions. Pour off plasma into aliquot tube.
Specimen Stability Information
Specimen Type Temperature Time
​Plasma ​ ​Frozen (preferred) ​30 days
​Refrigerate ​7 days
Rejection Criteria
Gross hemolysis​OK
Gross lipemia​OK
Interference

Nonsymptomatic EBV viremia or viral shedding may occur occasionally in healthy individuals. Therefore, this test should be used only for patients with a clinical history and symptoms consistent with EBV infection, and must be interpreted in the context of patient's clinical history, signs and symptoms. This test should not be used to screen asymptomatic patients.

Only plasma specimens are acceptable for testing with this assay, and lipemic plasma specimens may result in reduced assay sensitivity or assay failure.

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Friday ​2 days ​Real-Time Polymerase Chain Reaction (PCR) followed by Minor Groove-Binding (MGB) Probe Hybridization
Reference Lab
Test Information

Serial determination of plasma specimens from organ transplant recipients may be necessary to monitor increasing (risk of development of posttransplant lymphoproliferative disorders: PTLD) or decreasing (treatment efficacy) levels of Epstein-Barr virus (EBV) DNA.

Due to potential differences in assay performance, serial monitoring of a patient's EBV viral load should be performed using the same exact assay.

Reference Range Information
Performing Location Reference Range
​Mayo Medical Laboratories

​Undetected

Reportable Range: 100-5,000,000 IU/mL  (or 2.00-6.70 log IU/mL), with a limit of detection (based on a 95% detection rate) at 45 IU/mL (1.65 log IU/mL).

Interpretation

​Increasing levels of Epstein-Barr virus (EBV) DNA in serial plasma specimens of a given organ transplant recipient may indicate possible development of posttransplant lymphoproliferative disorder (PTLD).

An "Undetected" result indicates that EBV DNA is not detected in the plasma specimen (see Cautions). If clinically indicated, repeat testing in 1 to 2 months is recommended.

A result of "<100 IU/mL" indicates that the EBV DNA level present in the plasma specimen is below 100 IU/mL (or 2.00 log IU/mL), and the assay cannot accurately quantify the EBV DNA present below this level.

A quantitative value (reported in IU/mL and log IU/mL) indicates the EBV DNA level (ie, viral load) present in the plasma specimen.

A result of ">5,000,000 IU/mL" indicates that the EBV DNA level present in the plasma specimen is above 5,000,000 IU/mL (6.70 log IU/mL), and this assay cannot accurately quantify the EBV DNA present above this level.

An "Inconclusive" result indicates that the presence or absence of EBV DNA in the plasma specimen could not be determined with certainty after repeat testing in the laboratory, possibly due to PCR inhibition or presence of interfering substance. Submission of a new specimen for testing is recommended if clinically indicated.

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87799 ​1
Synonyms/Keywords
EBV, Infectious Mononucleosis, Viral Load, Post Transplant Lymphoproliferative Disorder (PTLD)
Ordering Applications
Ordering Application Description
​Centricity Epstein-Barr Virus DNA Detection/Quant (EBVQU)
​Cerner Epstein-Barr Virus DNA Detection/Quant (EBVQU)
​COM Epstein-Barr Virus DNA Detection/Quant (EBVQU)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Plasma ​EDTA Lavender Top Tube (LTT) ​1.5 mL ​0.8 mL
Collection Processing
Centrifuge blood collection tube per collection tube manufacturer's instructions. Pour off plasma into aliquot tube.
Specimen Stability Information
Specimen Type Temperature Time
​Plasma ​ ​Frozen (preferred) ​30 days
​Refrigerate ​7 days
Rejection Criteria
Gross hemolysis​OK
Gross lipemia​OK
Interference

Nonsymptomatic EBV viremia or viral shedding may occur occasionally in healthy individuals. Therefore, this test should be used only for patients with a clinical history and symptoms consistent with EBV infection, and must be interpreted in the context of patient's clinical history, signs and symptoms. This test should not be used to screen asymptomatic patients.

Only plasma specimens are acceptable for testing with this assay, and lipemic plasma specimens may result in reduced assay sensitivity or assay failure.

Useful For

Diagnosis of EBV-associated infectious mononucleosis in individuals with equivocal or discordant Epstein-Barr virus (EBV) serologic marker test results.

Diagnosis of posttransplant lymphoproliferative disorders (PTLD), especially in EBV-seronegative organ transplant recipients receiving antilymphocyte globulin for induction immunosuppression and OKT-3 treatment for early organ rejection.

Monitoring progression of EBV-associated PTLD in organ transplant recipients.

Reference Range Information
Performing Location Reference Range
​Mayo Medical Laboratories

​Undetected

Reportable Range: 100-5,000,000 IU/mL  (or 2.00-6.70 log IU/mL), with a limit of detection (based on a 95% detection rate) at 45 IU/mL (1.65 log IU/mL).

Interpretation

​Increasing levels of Epstein-Barr virus (EBV) DNA in serial plasma specimens of a given organ transplant recipient may indicate possible development of posttransplant lymphoproliferative disorder (PTLD).

An "Undetected" result indicates that EBV DNA is not detected in the plasma specimen (see Cautions). If clinically indicated, repeat testing in 1 to 2 months is recommended.

A result of "<100 IU/mL" indicates that the EBV DNA level present in the plasma specimen is below 100 IU/mL (or 2.00 log IU/mL), and the assay cannot accurately quantify the EBV DNA present below this level.

A quantitative value (reported in IU/mL and log IU/mL) indicates the EBV DNA level (ie, viral load) present in the plasma specimen.

A result of ">5,000,000 IU/mL" indicates that the EBV DNA level present in the plasma specimen is above 5,000,000 IU/mL (6.70 log IU/mL), and this assay cannot accurately quantify the EBV DNA present above this level.

An "Inconclusive" result indicates that the presence or absence of EBV DNA in the plasma specimen could not be determined with certainty after repeat testing in the laboratory, possibly due to PCR inhibition or presence of interfering substance. Submission of a new specimen for testing is recommended if clinically indicated.

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Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Medical Laboratories ​Monday through Friday ​2 days ​Real-Time Polymerase Chain Reaction (PCR) followed by Minor Groove-Binding (MGB) Probe Hybridization
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87799 ​1
For most current information refer to the Marshfield Laboratory online reference manual.