Specimen must be drawn WITHOUT a tourniquet and WITHOUT hand clenching.
Specimens are drawn at baseline and at 1,3,5, 10 and 20 minutes post exercise.
Centrifuge in refrigerated centrifuge immediately and remove plasma to plastic tube within 15 minutes.
Send all blood specimens to the laboratory as a group at the end of the test. After collection, each sample should be kept on wet ice until analyzed.