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23165 Bordetella by PCR

Bordetella by PCR
Test Code: BORDPCR
Synonyms/Keywords
​​Bordetella, Pertussis PCR, Whooping Cough, Parapertussis PCR
Useful For
​Bordetella by PCR is the preferred method for the detection of Bordetella pertussis and Bordetella parapertussis, since it is more sensitive than culture, and is less subject to the effects of delayed transport.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No
Naspharyngeal swab
 
 Universal Transport media- UTM
 Universal Viral Transport (UVT, BD) Copan ESwab (Liquid Amies)
 ​ 1 swab​ 1 swab​
Collection Processing Instructions

​Submit one flexible shaft nasopharyngeal swab of Dacron or Rayon in universal transport media.  Calcium alginate is NOT acceptable for PCR. Send sample to lab on ice.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Acceptable Specimen Types
​Nasopharyngeal Swabs
Specimen Stability Information
Specimen Type Temperature Time​​
Swab​ ​ Refrigerate​ (2-8 degrees C) Up to ​7 days
​Frozen at < or = -70 degrees C​Up to 5 months
Rejection Criteria
Calcium alginate swab
​Wooden shaft swab
Charcoal swab​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield
Monday through Sunday  
6-8 hours
 Real Time PCR, IVD
Test Information
BORDPCR is a real-time polymerase chain reaction (PCR) based qualitative in vitro diagnostic test for the direct detection and identification of Bordetella pertussis and Bordetella parapertussis nucleic acid in nasopharyngeal swab (NPS) specimens obtained from individuals suspected of having a respiratory tract infection attributable to B. pertussis or B. parapertussis.
Reference Range Information
Performing Location Reference Range
​Marshfield

Negative for Bordetella pertussis by PCR.

Negative for Bordetella parapertussis by PCR.​

Interpretation
​Cross-reactivity with Bordetella holmesii may occur with the Bordetella pertussis polymerase chain reaction (PCR) assay. While the prevalence of B. holmesii is relatively low, it has been associated with pertussis-like symptoms.  

Bordetella is a Category 1 reportable disease.

All positive Bordetella results are called to provider.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87801 ​ ​ ​ ​
Synonyms/Keywords
​​Bordetella, Pertussis PCR, Whooping Cough, Parapertussis PCR
Ordering Applications
Ordering Application Description
​Cerner ​Bordetella pertussis by PCR
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No
Naspharyngeal swab
 
 Universal Transport media- UTM
 Universal Viral Transport (UVT, BD) Copan ESwab (Liquid Amies)
 ​ 1 swab​ 1 swab​
Collection Processing

​Submit one flexible shaft nasopharyngeal swab of Dacron or Rayon in universal transport media.  Calcium alginate is NOT acceptable for PCR. Send sample to lab on ice.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Acceptable Specimen Types
​Nasopharyngeal Swabs
Specimen Stability Information
Specimen Type Temperature Time​​
Swab​ ​ Refrigerate​ (2-8 degrees C) Up to ​7 days
​Frozen at < or = -70 degrees C​Up to 5 months
Rejection Criteria
Calcium alginate swab
​Wooden shaft swab
Charcoal swab​
Useful For
​Bordetella by PCR is the preferred method for the detection of Bordetella pertussis and Bordetella parapertussis, since it is more sensitive than culture, and is less subject to the effects of delayed transport.
Reference Range Information
Performing Location Reference Range
​Marshfield

Negative for Bordetella pertussis by PCR.

Negative for Bordetella parapertussis by PCR.​

Interpretation
​Cross-reactivity with Bordetella holmesii may occur with the Bordetella pertussis polymerase chain reaction (PCR) assay. While the prevalence of B. holmesii is relatively low, it has been associated with pertussis-like symptoms.  

Bordetella is a Category 1 reportable disease.

All positive Bordetella results are called to provider.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield
Monday through Sunday  
6-8 hours
 Real Time PCR, IVD
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87801 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.