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23380 HLA Antibody Detection and Identification

HLA Antibody Detection and Identification
Test Code: MISC
Synonyms/Keywords
​Ref Lab Code: 2222, PRA Mini Screen, Platelet Reactive Antibody
Useful For
​Solid organ transplantation.
If ordering HLA matched platelets, refer to "HLA Matched Platelet" for correct test codes.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​Clot tube ​Red Top Tube (RTT) ​ ​10 mL ​5 mL ​
Collection Processing Instructions
Do not separate serum from clot. Submit sample at room temperature. Collect pre-dialysis (if applicable).​
Specimen Stability Information
Specimen Type Temperature Time
​Clot tube Room temperature​ ​7 days
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Blood Center of Wisconsin    ​ Undefined​

​7 days for Detection

10 days for Identification

 Flow Cytometry​
Reference Lab
Versiti/Blood Center of Wisconsin
Test Information
Always includes HLA Antibody Detection. If HLA Antibody Screen is positive, also includes HLA Antibody Identification - Class I and/or HLA Antibody Identification - Class II.
The HLA Antibody Detection and Identification must be completed before HLA matched platelets will be available from the Blood Center.
The Blood Center may not send HLA matched platelets when the patient has negative HLA Antibody Detection and Identification results. Transfusion Services will be notified and will send an HLA-Matched Platelets Progress Note/Sticker indicating that the Blood Center is NOT collecting HLA-matched products.​
Reference Range Information
No reference ranges are available.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86828​ ​ ​ ​Detection ​
​86832 ​ ​ ​Identification (Class I) ​
​86833 ​ ​ ​Identification (Class II) ​
Synonyms/Keywords
​Ref Lab Code: 2222, PRA Mini Screen, Platelet Reactive Antibody
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​Clot tube ​Red Top Tube (RTT) ​ ​10 mL ​5 mL ​
Collection Processing
Do not separate serum from clot. Submit sample at room temperature. Collect pre-dialysis (if applicable).​
Specimen Stability Information
Specimen Type Temperature Time
​Clot tube Room temperature​ ​7 days
Useful For
​Solid organ transplantation.
If ordering HLA matched platelets, refer to "HLA Matched Platelet" for correct test codes.
Reference Range Information
No reference ranges are available.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Blood Center of Wisconsin    ​ Undefined​

​7 days for Detection

10 days for Identification

 Flow Cytometry​
Reference Lab
Versiti/Blood Center of Wisconsin
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86828​ ​ ​ ​Detection ​
​86832 ​ ​ ​Identification (Class I) ​
​86833 ​ ​ ​Identification (Class II) ​
For most current information refer to the Marshfield Laboratory online reference manual.