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25073 FLT3 Mutation Analysis, Varies (FLT)

FLT3 Mutation Analysis, Varies (FLT)
Test Code: FLT3SO
Synonyms/Keywords
FLT
FLT3
FLT3-ITD
FLT3 coding for D835
Useful For

A prognostic indicator in some patients with acute myeloid leukemia

This test should not be used to monitor residual disease following treatment.

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Collect one of the following specimens:
​Whole Blood ​EDTA Lavender Top Tube (LTT) ​ 3 mL ​ ​
​Bone Marrow ​EDTA Lavender Top Tube (LTT) ​ ​2 mL ​ ​
Collection Processing Instructions

​1. Invert tube several times to mix blood or bone marrow.

2. Send specimen in original tube.  Do not aliquot.

3.  Label specimen as blood or bone marrow.

Specimen must arrive within 7 days of collection.

The following information is required:

1. Pertinent clinical history

2. Clinical or morphologic suspicion

3. Date and time of collection

4. Specimen source

Specimen Stability Information
Specimen Type Temperature Time
​Whole Blood or Bone Marrow ​ ​Ambient (preferred) ​7 days
​Refrigerated ​7 days
Rejection Criteria

Grossly Hemolyzed

Bone marrow biopsies

Slides

Paraffin shavings

Heparinized samples

Moderately to severely clotted

Interference

​This test is not designed for monitoring residual disease following treatment and the following should be noted: the sensitivity of the test is less than other methods designed for residual disease testing and there have been several reports of FLT3 variants being lost or gained in neoplastic cells following treatment.

Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation

Mayo Clinic Laboratories

 ​Monday through Saturday ​3 to 6 days Polymerase Chain Reaction (PCR)/Capillary Electrophoresis
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
Performing Location Reference Range
Mayo Clinic Laboratories
 An interpretive report will be provided.
Interpretation

An interpretive report will be issued indicating whether the FLT3 internal tandem duplication (ITD), D835 alteration, or both were detected.

Variant status will be indicated as positive or negative. If ITD positive, an allelic ratio will be reported.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​81245 ​ ​1 ​FLT3 Mutation Detection ​
81246 ​ ​1 ​Tyrosine Kinase Domain (TKD) variants (D835) ​
Synonyms/Keywords
FLT
FLT3
FLT3-ITD
FLT3 coding for D835
Ordering Applications
Ordering Application Description
​Centricity ​FLT3 Mutation Detection, PCR
​Cerner ​FLT3 Mutation Detection, PCR
​COM ​FLT3 Mutation Detection, PCR
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Collect one of the following specimens:
​Whole Blood ​EDTA Lavender Top Tube (LTT) ​ 3 mL ​ ​
​Bone Marrow ​EDTA Lavender Top Tube (LTT) ​ ​2 mL ​ ​
Collection Processing

​1. Invert tube several times to mix blood or bone marrow.

2. Send specimen in original tube.  Do not aliquot.

3.  Label specimen as blood or bone marrow.

Specimen must arrive within 7 days of collection.

The following information is required:

1. Pertinent clinical history

2. Clinical or morphologic suspicion

3. Date and time of collection

4. Specimen source

Specimen Stability Information
Specimen Type Temperature Time
​Whole Blood or Bone Marrow ​ ​Ambient (preferred) ​7 days
​Refrigerated ​7 days
Rejection Criteria

Grossly Hemolyzed

Bone marrow biopsies

Slides

Paraffin shavings

Heparinized samples

Moderately to severely clotted

Interference

​This test is not designed for monitoring residual disease following treatment and the following should be noted: the sensitivity of the test is less than other methods designed for residual disease testing and there have been several reports of FLT3 variants being lost or gained in neoplastic cells following treatment.

Useful For

A prognostic indicator in some patients with acute myeloid leukemia

This test should not be used to monitor residual disease following treatment.

Reference Range Information
Performing Location Reference Range
Mayo Clinic Laboratories
 An interpretive report will be provided.
Interpretation

An interpretive report will be issued indicating whether the FLT3 internal tandem duplication (ITD), D835 alteration, or both were detected.

Variant status will be indicated as positive or negative. If ITD positive, an allelic ratio will be reported.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation

Mayo Clinic Laboratories

 ​Monday through Saturday ​3 to 6 days Polymerase Chain Reaction (PCR)/Capillary Electrophoresis
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​81245 ​ ​1 ​FLT3 Mutation Detection ​
81246 ​ ​1 ​Tyrosine Kinase Domain (TKD) variants (D835) ​
For most current information refer to the Marshfield Laboratory online reference manual.