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25223 Neonatal Screen (281)

Neonatal Screen (281)
Test Code: NEO
Synonyms/Keywords
PKU-Newborn Screening, CAH Trypsinogen Screen, Newborn Screening
Useful For

Detect babies with congenital disorders​

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​Capillary Blood ​Filter Paper Specimen Cards ​ ​5 Circles completely filled ​ ​
Collection Processing Instructions

​5 filter paper circles completely filled with free flowing capillary blood on special filter paper specimen cards.  Completely air dry card in horizontal position before sending to the laboratory.

Blood must dry for a minimum of 3 hrs. Dried specimens should be transported to State Laboratory of Hygiene within 24 hours (the State Laboratory of Hygiene provides a next-day courier delivery service).

Specimen Stability Information
Specimen Type Temperature
Capillary Blood on ​Filter Paper Specimen Cards Ambient
Rejection Criteria

Circles containing clots or less than four completely filled dried blood circles on card.

Incomplete saturation, uneven saturation, layed specimen, plasma separated from red blood cells, specimen damaged, blood clots within the circle of blood, contamination with water, formula, urine, tissue fluid, antiseptic, etc.

Specimen received more than seven days after collection, specimen not allowed to dry before mailing, outdated collection card, large folds or creases through the blood circles, defective specimen collection card, blood observed on tan cover.

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Wisconsin State Lab of Hygiene​ Monday through Saturday​

​Same day/1 day - normal results

Up to 4 days - abnormal results

 Enzymatic assay, immunoreaction assay, electrophoresis, high performance liquid chromography, tandem mass spectrometry, polymerase chain reaction (PCR), real-time PCR, digital droplet PCR, and sequencing technology
Reference Lab
Wisconsin State Lab of Hygiene
Test Information

Screen for the following disorders: arginosuccinic acidemia, biotinidase deficiency, citrullinemia (I & II), congenital adrenal hyperplasia, congenital hypothyroidism, cystic fibrosis, fatty acid oxidation disorders (12), galactosemia, sickle cell disease, hemoglobin S-Beta thalessemia, hemoglobin S-C disease, hemoglobin E-Beta thalessemia, Beta thalassemia, homocystinuria, hypermethioninemia, maple syrup disease, organic acidemia (12), phenylketonuria (PKU), hyperphenylalaninemia, tyrosinemia (I, II & III), severe combined immune deficiency (SCID), and spinal muscular atrophy (SMA).

Reference Range Information
Interpretive Report
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​84999 ​ ​ ​ ​
Synonyms/Keywords
PKU-Newborn Screening, CAH Trypsinogen Screen, Newborn Screening
Ordering Applications
Ordering Application Description
​Centricity ​Neo-Natal Screen
​Cerner ​Neo-Natal Screen
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​Capillary Blood ​Filter Paper Specimen Cards ​ ​5 Circles completely filled ​ ​
Collection Processing

​5 filter paper circles completely filled with free flowing capillary blood on special filter paper specimen cards.  Completely air dry card in horizontal position before sending to the laboratory.

Blood must dry for a minimum of 3 hrs. Dried specimens should be transported to State Laboratory of Hygiene within 24 hours (the State Laboratory of Hygiene provides a next-day courier delivery service).

Specimen Stability Information
Specimen Type Temperature
Capillary Blood on ​Filter Paper Specimen Cards Ambient
Rejection Criteria

Circles containing clots or less than four completely filled dried blood circles on card.

Incomplete saturation, uneven saturation, layed specimen, plasma separated from red blood cells, specimen damaged, blood clots within the circle of blood, contamination with water, formula, urine, tissue fluid, antiseptic, etc.

Specimen received more than seven days after collection, specimen not allowed to dry before mailing, outdated collection card, large folds or creases through the blood circles, defective specimen collection card, blood observed on tan cover.

Useful For

Detect babies with congenital disorders​

Reference Range Information
Interpretive Report
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Wisconsin State Lab of Hygiene​ Monday through Saturday​

​Same day/1 day - normal results

Up to 4 days - abnormal results

 Enzymatic assay, immunoreaction assay, electrophoresis, high performance liquid chromography, tandem mass spectrometry, polymerase chain reaction (PCR), real-time PCR, digital droplet PCR, and sequencing technology
Reference Lab
Wisconsin State Lab of Hygiene
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​84999 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.