Allergen, Mushroom, IgE (MUSH)

Test Code: ALMSHSO

Overview
Ordering
Specimen
Performing
Clinical/Interpretive
Contacts
Coding

Synonyms/Keywords

Agaricus bisporus, Agaricus hortensis, Buttom Mushroom, Champignon, Common Mushroom, Cultivated Mushroom, Table Mushroom, White Mushroom,

Useful For

Establishing the diagnosis of an allergy to mushroom

Defining the allergen responsible for eliciting signs and symptoms

Identifying allergens:

- Responsible for allergic disease and/or anaphylactic episode

- To confirm sensitization prior to beginning immunotherapy

- To investigate the specificity of allergic reactions to insect venom allergens, drugs, or chemical allergens

Testing for IgE antibodies is not useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists, or in patients in whom the medical management does not depend upon identification of allergen specificity.

Specimen Requirements

Specimen Type	Preferred Container/Tube	Acceptable Container/Tube	Specimen Volume	Specimen Minimum Volume (allows for 1 repeat)	Pediatric Minimum Volume (no repeat)
Serum	Serum Separator Tube (SST)	Red Top Tube (RTT)	0.5 mL for each 5 allergens requested	0.3 mL

Collection Processing Instructions

For 1 allergen: 0.3 mL
For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace

Specimen Stability Information

Specimen Type	Temperature	Time
Serum	Refrigerated (preferred)	14 days
Serum	Frozen	90 days

Interference

Some individuals with clinically insignificant sensitivity to allergens may have measureable levels of IgE antibodies in serum, and results must be interpreted in the clinical context.

False-positive resuts for IgE antibodies may occur in patients with markedly elevated serum IgE (.2,500 kU/L) due to nonspecific binding to allergen solid phases.

Performing Laboratory Information

Performing Location	Day(s) Test Performed	Report Available	Methodology/Instrumentation
Mayo Clinic Laboratories	Monday through Friday	1 to 3 days	Fluorescence Enzyme Immunoassay (FEIA)

Reference Lab

Mayo Clinic Laboratories

Reference Range Information

Performing Location

Reference Range

Mayo Clinic Laboratories

Class	IgE kU/L	Interpretation
0	<0.10	Negative
0/1	0.10-0.34	Borderline/equivocal
1	0.35-0.69	Equivocal
2	0.70-3.49	Positive
3	3.50-17.4	Positive
4	17.5-49.9	Strongly positive
5	50.0-99.9	Strongly positive
6	> or =100	Strongly positive

Reference values apply to all ages.

Interpretation

Detection of IgE antibodies in serum (Class 1 or greater) indicates an increased likelihood of allergic disease as opposed to other etiologies and defines the allergens that may be responsible for eliciting signs and symptoms.

The level of IgE antibodies in serum varies directly with the concentration of IgE antibodies expressed as a class score or kU/L.

Outreach CPTs

CPT	Modifier (if needed)	Quantity	Description	Comments
86003		1

Classification