You may be trying to access this site from a secured browser on the server. Please enable scripts and reload this page.
Turn on more accessible mode
Turn off more accessible mode
Skip Ribbon Commands
Skip to main content
Turn off Animations
Turn on Animations
Sign In
This page location is:
Lab Test Reference Manual
Human Reference Manual
Pages
26033
Lab Test Reference Manual
Human Reference Manual
Currently selected
26033
Vitamin B-12/Folate
Marshfield Lab Public WebSite
Marshfield Clinic Public WebSite
It looks like your browser does not have JavaScript enabled. Please turn on JavaScript and try again.
Search
Test Code
Laboratory Section
All
Test Category
All
Browse By Test Name
#
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
Additional Information
Libraries
Newsletter Document Library
Regional Menus Pages
Lists
Lab Clinical Practice Guidelines
Antimicrobial Susceptibility Panels
Toxicology Information
Urine Preservatives
Requisition Forms and Instructions
Testing
Newsletter Links
Newsletters 2014
Newsletters 2015
Newsletters 2016
Newsletters 2017
Newsletters 2018
Cumulative Antibiogram Reports
testz
Human Test Code IDs
Recent
Newsletters 2025
Newsletters 2024
Newsletters 2023
Newsletters 2022
Antibiogram-PDFs
Site Contents
Vitamin B-12/Folate
Test Code: B/F
Overview
Ordering
Specimen
Performing
Clinical/Interpretive
Contacts
Coding
Synonyms/Keywords
Synonyms, Keywords
Vitamin B-12, B12, Cyanocobalamin, Folate, Folic Acid
Useful For
Useful For
Investigation of macrocytic anemia, workup of deficiencies seen in megaloblastic anemias, investigation of suspected folate deficiency.
Specimen Requirements
Specimen Requirements
Fasting Required
Specimen Type
Preferred Container/Tube
Acceptable Container/Tube
Specimen Volume
Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No
Serum
Serum Separator Tube (SST)
Red Top Tube (RTT)
1.0 mL
0.8 mL
0.6 mL
Collection Processing Instructions
Collection Processing
Refrigerate off the cells up to 4 hours. If analysis will be delayed longer than 4 hours, freeze the sample. Avoid repeated freezing and thawing of serum specimen. High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with the Folate assay, therefore it is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Specimen Stability Information
Specimen Stability Information
Specimen Type
Temperature
Time
Serum
Refrigerated off cells
< 4 hours
Frozen
2 months
Rejection Criteria
Rejection Criteria
More than Trace Hemolysis
Interference
Interference
Folate: Human Anti-Murine Antibodies (HAMA) may develop leading to test interference in patients receiving murine-based agents for chemotherapy or tumor-imaging studies. Likewise, Fluorescein used in retinal angiography will interfere. High doses of exogenous biotin (also termed Vitamin B7, Vitamin H, or Coenzyme R) may interfere with this assay.
Performing Laboratory Information
Performing Laboratory Information
Performing Location
Day(s) Test Performed
Analytical Time
Methodology/Instrumentation
Marshfield
Monday through Sunday
Less than 2 hours
Competitive Immunoassay using Direct Chemiluminescent Technology/Siemens Centaur
Reference Range Information
Reference Range Information
Performing Location
Reference Range
Marshfield
Folate: >= 3.4 ng/mL
Vitamin B-12: 160-1000 pg/mL
Outreach CPTs
Outreach CPT Codes
CPT
Modifier
(if needed)
Quantity
Description
Comments
82746
1
Folate
82607
1
Vitamin B-12
Synonyms/Keywords
Synonyms, Keywords
Vitamin B-12, B12, Cyanocobalamin, Folate, Folic Acid
Ordering Applications
Ordering Applications
Ordering Application
Description
COM
Vitamin B-12/Folate
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Requirements
Fasting Required
Specimen Type
Preferred Container/Tube
Acceptable Container/Tube
Specimen Volume
Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No
Serum
Serum Separator Tube (SST)
Red Top Tube (RTT)
1.0 mL
0.8 mL
0.6 mL
Collection Processing
Collection Processing
Refrigerate off the cells up to 4 hours. If analysis will be delayed longer than 4 hours, freeze the sample. Avoid repeated freezing and thawing of serum specimen. High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with the Folate assay, therefore it is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Specimen Stability Information
Specimen Stability Information
Specimen Type
Temperature
Time
Serum
Refrigerated off cells
< 4 hours
Frozen
2 months
Rejection Criteria
Rejection Criteria
More than Trace Hemolysis
Interference
Interference
Folate: Human Anti-Murine Antibodies (HAMA) may develop leading to test interference in patients receiving murine-based agents for chemotherapy or tumor-imaging studies. Likewise, Fluorescein used in retinal angiography will interfere. High doses of exogenous biotin (also termed Vitamin B7, Vitamin H, or Coenzyme R) may interfere with this assay.
Useful For
Useful For
Investigation of macrocytic anemia, workup of deficiencies seen in megaloblastic anemias, investigation of suspected folate deficiency.
Reference Range Information
Reference Range Information
Performing Location
Reference Range
Marshfield
Folate: >= 3.4 ng/mL
Vitamin B-12: 160-1000 pg/mL
For more information visit:
http://labtestsonline.org
Performing Laboratory Information
Performing Laboratory Information
Performing Location
Day(s) Test Performed
Analytical Time
Methodology/Instrumentation
Marshfield
Monday through Sunday
Less than 2 hours
Competitive Immunoassay using Direct Chemiluminescent Technology/Siemens Centaur
For billing questions, see Contacts
Outreach CPTs
Outreach CPT Codes
CPT
Modifier
(if needed)
Quantity
Description
Comments
82746
1
Folate
82607
1
Vitamin B-12
Return To Top
For most current information refer to the Marshfield Laboratory online reference manual.