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26078 Bullous Pemphigoid, BP180 & BP230, IgG Antibodies, Serum (BPAB)

Bullous Pemphigoid, BP180 & BP230, IgG Antibodies, Serum (BPAB)
Test Code: BPSO
Synonyms/Keywords

BP 180

BP 230

Bullous Pemphigoid

Useful For

Initial screening test in the diagnosis of bullous pemphigoid and its variants.

Complementing the standard serum test of indirect immunofluorescence utilitzing monkey esophagus substrate and human salt-split skin substrate (CIFS/Cutaneous Immunofluorescence Antibodies (IgG), Serum) 

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Serum ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​1 mL ​0.5 mL
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​ ​ ​Refrigerate (preferred) ​14 days
​Frozen ​30 days
​Ambient ​14 days
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories ​Varies ​1 to 5 days ​Enzyme-Linked Immunosorbent Assay (ELISA)
Reference Lab
Reference Range Information

BULLOUS PEMPHIGOID 180

<20 RU/mL (negative)

> or =20 RU/mL (positive)

BULLOUS PEMPHIGOID 230

<20 RU/mL (negative)

> or =20 RU/mL (positive)

Interpretation

Antibodies to bullous pemphigoid (BP) BP180 and BP230 have been shown to be present in most patients with pemphigoid. Adequate sensitivities and specificity for disease are documented and Mayo’s experience demonstrates a very good correlation between BP180 and BP230 results and the presence of pemphigoid (see Supportive Data). However, in those patients strongly suspected to have pemphigoid, either by clinical findings or by routine biopsy, and in whom the BP180/BP230 assay is negative, follow-up testing by CIFS / Cutaneous Immunofluorescence Antibodies (IgG), Serum is recommended.

Antibody titer correlates with disease activity in many patients. Patients with severe disease can usually be expected to have high titers of antibodies to BP. Titers are expected to decrease with clinical improvement.

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​83516 2
Synonyms/Keywords

BP 180

BP 230

Bullous Pemphigoid

Ordering Applications
Ordering Application Description
​Centricity ​Bullous Pemphigoid 180/230 Abs
​Cerner ​Bullous Pemphigoid 180/230 Abs
​COM ​Bullous Pemphigoid 180/230 Abs
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Serum ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​1 mL ​0.5 mL
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​ ​ ​Refrigerate (preferred) ​14 days
​Frozen ​30 days
​Ambient ​14 days
Useful For

Initial screening test in the diagnosis of bullous pemphigoid and its variants.

Complementing the standard serum test of indirect immunofluorescence utilitzing monkey esophagus substrate and human salt-split skin substrate (CIFS/Cutaneous Immunofluorescence Antibodies (IgG), Serum) 

Reference Range Information

BULLOUS PEMPHIGOID 180

<20 RU/mL (negative)

> or =20 RU/mL (positive)

BULLOUS PEMPHIGOID 230

<20 RU/mL (negative)

> or =20 RU/mL (positive)

Interpretation

Antibodies to bullous pemphigoid (BP) BP180 and BP230 have been shown to be present in most patients with pemphigoid. Adequate sensitivities and specificity for disease are documented and Mayo’s experience demonstrates a very good correlation between BP180 and BP230 results and the presence of pemphigoid (see Supportive Data). However, in those patients strongly suspected to have pemphigoid, either by clinical findings or by routine biopsy, and in whom the BP180/BP230 assay is negative, follow-up testing by CIFS / Cutaneous Immunofluorescence Antibodies (IgG), Serum is recommended.

Antibody titer correlates with disease activity in many patients. Patients with severe disease can usually be expected to have high titers of antibodies to BP. Titers are expected to decrease with clinical improvement.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories ​Varies ​1 to 5 days ​Enzyme-Linked Immunosorbent Assay (ELISA)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​83516 2
For most current information refer to the Marshfield Laboratory online reference manual.