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26473 Antimicrobial Susceptibility, Acid-Fast Bacilli, Slowly Growing, Varies (MMLSG)

Antimicrobial Susceptibility, Acid-Fast Bacilli, Slowly Growing, Varies (MMLSG)
Test Code: MISC
Synonyms/Keywords

​​Acid-Fast Bacilli (AFB), AFB (Acid-Fast Bacilli), Antibiotic Susceptibility, Bacillus, Acid-Fast, MIC (Minimum Inhibitory Concentration), Minimum Inhibitory Concentration (MIC), Mycobacteria Antimicrobial Susceptibility (MIC) (Minimum Inhibitory Concentration), Mycobacterium marinum Susceptibility Testing, Mycobacterium kansasii Susceptibility Testing, Mycobacterium avium complex Susceptibility Testing, Slow Growing Mycobacteria to Antimicrobial Agents, Susceptibility Testing

Useful For

Determination of resistance of slowly growing mycobacteria to antimicrobial agents

When this test is ordered, susceptibility for slow growers will be performed at an additional charge. Antimicrobials are tested and reported using the Clinical and Laboratory Standards Institute (CLSI) guidelines.(1, 2)

Antimicrobials tested for Mycobacterium avium complex: amikacin, clarithromycin, clofazimine, linezolid, and moxifloxacin.

Antimicrobials tested for all other slowly growing Mycobacterium species: amikacin, clarithromycin, clofazimine, ciprofloxacin, doxycycline, linezolid, minocycline, moxifloxacin, rifabutin, rifampin, and trimethoprim /sulfamethoxazole.

Clofazimine interpretive criterion is not available.

Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​Organism​Middlebrook 7H10 agar slant or other appropriate media​​Isolate​​
Collection Processing Instructions

Specimen source and organism identification are required.

Organism must be in pure culture, actively growing. 

CTB / Mycobacteria and Nocardia Culture, Varies or CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies must also be ordered and will be charged separately unless identification of organism is provided.

Specimen Stability Information
Specimen TypeTemperature
​Varies ​​Ambient (preferred)
​Refrigerated
Rejection Criteria
Agar plate
Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Varies​12 to 35 days​Minimum Inhibitory Concentration (MIC) by Microtiter Broth Dilution Method
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​Interpretive Report will be provided.
Interpretation

​Results are reported as the minimum inhibitory concentration in micrograms/mL.

Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​87186​​1​​
Synonyms/Keywords

​​Acid-Fast Bacilli (AFB), AFB (Acid-Fast Bacilli), Antibiotic Susceptibility, Bacillus, Acid-Fast, MIC (Minimum Inhibitory Concentration), Minimum Inhibitory Concentration (MIC), Mycobacteria Antimicrobial Susceptibility (MIC) (Minimum Inhibitory Concentration), Mycobacterium marinum Susceptibility Testing, Mycobacterium kansasii Susceptibility Testing, Mycobacterium avium complex Susceptibility Testing, Slow Growing Mycobacteria to Antimicrobial Agents, Susceptibility Testing

Ordering Applications
Ordering ApplicationDescription
​COM​Antimicrobial Suscep, AF Bacilli, Slow Grow (MMLSG)
​Cerner​Antimicrobial Susceptibility, Acid-Fast Bacilli, Slowing Growing, Varies (MMLSG)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​Organism​Middlebrook 7H10 agar slant or other appropriate media​​Isolate​​
Collection Processing

Specimen source and organism identification are required.

Organism must be in pure culture, actively growing. 

CTB / Mycobacteria and Nocardia Culture, Varies or CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies must also be ordered and will be charged separately unless identification of organism is provided.

Specimen Stability Information
Specimen TypeTemperature
​Varies ​​Ambient (preferred)
​Refrigerated
Rejection Criteria
Agar plate
Useful For

Determination of resistance of slowly growing mycobacteria to antimicrobial agents

When this test is ordered, susceptibility for slow growers will be performed at an additional charge. Antimicrobials are tested and reported using the Clinical and Laboratory Standards Institute (CLSI) guidelines.(1, 2)

Antimicrobials tested for Mycobacterium avium complex: amikacin, clarithromycin, clofazimine, linezolid, and moxifloxacin.

Antimicrobials tested for all other slowly growing Mycobacterium species: amikacin, clarithromycin, clofazimine, ciprofloxacin, doxycycline, linezolid, minocycline, moxifloxacin, rifabutin, rifampin, and trimethoprim /sulfamethoxazole.

Clofazimine interpretive criterion is not available.

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​Interpretive Report will be provided.
Interpretation

​Results are reported as the minimum inhibitory concentration in micrograms/mL.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Varies​12 to 35 days​Minimum Inhibitory Concentration (MIC) by Microtiter Broth Dilution Method
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​87186​​1​​
For most current information refer to the Marshfield Laboratory online reference manual.