Skip Ribbon Commands
Skip to main content
Sign In
Navigate Up

26514 Cytomegalovirus (CMV) Molecular Detection, PCR, Varies (CMVPV)

Cytomegalovirus (CMV) Molecular Detection, PCR, Varies (CMVPV)
Test Code: CMVPVSO
Synonyms/Keywords

​​​CMV Detection by Real-Time PCR

Cytomegalovirus, PCR

LightCycler CMV

PCR (Polymerase Chain Reaction)​

Test Components

​Cytomegalovirus PCR

Useful For

​Rapid qualitative detection of cytomegalovirus (CMV) DNA

This test is not intended for the monitoring of CMV disease progression.

This test should not be used to screen asymptomatic patients.

Specimen Requirements

Specimen TypePreferred Container/TubeAcceptable Container/Tube
Specimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)

​Submit only 1 of the following specimens:​​​​ ​ ​ ​ ​ ​

​
Body Fluid

Sources: Spinal, pleural, peritoneal, ascites, pericardial, amniotic, or ocular .

Collection Instructions: Do not centrifuge.

​Sterile, screwcap, 5-mL aliquot tube
​Sterile container
​0.5 mL
Ocular Fluid, and Spinal Fluid:
0.3 mL

Body Fluid (pleural, peritoneal, ascites, pericardial):
 0.5 mL​
​
​

Upper respiratory tract fluid

Sources: nasopharyngeal aspirate or washing

Collection Instructions: Do not centrifuge.

Note:  for lower respiratory tract specimens,order test code:  CMVLRSO

​Sterile, screwcap, 5-mL aliquot tube
​Sterile container
​1.5 mL
​1.0 mL
​
​

Genital swab

Sources: Cervix, vagina, urethra, anal/rectal, or other genital sources.

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)


​Multimicrobe media (M4-RT) and ESwabs
​​​​
​

Swab

Sources: Dermal, eye, nasal, saliva, or throat.

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)​


​Multimicrobe media (M4-RT) and ESwabs
​​​​
​

Tissue

Sources: Brain, colon, kidney, liver, lung, etc.

Collection Instructions: Submit only fresh tissue in multimicrobe media (M4-RT) or a sterile container with 1 to 2 mL sterile saline


​Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, or M5)
​
​Entire collection
​2 x 2-mm biopsy
​
​Urine

Collection Instructions: Collect a random urine specimen.
​Sterile container
​​1.0 mL​
​0.3 mL​
​

Bone Marrow

Collection Instructions:
Send bone marrow in original tube.  Do not aliquot.​
​EDTA Lavender top (LTT)
​

​​0.5 mL
​
​
Collection Processing Instructions

​Specimen source is required.

For plasma specimens order CMVQN / Cytomegalovirus (CMV) DNA Detection and Quantification by Real-Time PCR, Plasma.

For lower respiratory tract specimens (bronchial washing, bronchoalveolar larvage, fluid/washings from lung, sputum, tracheal secretions, tracheal aspirates) order test code:  CMVLR/ Cytomegalovirus (CMV) Molecular Detection, PCR, Lower Respiratory.​​

Specimen Stability Information
Specimen TypeTemperature
Time
​​
Varies
​​
​Refrigerated (preferred)
​7 days
​Frozen
​7 days
Rejection Criteria

​Calcium alginate-tipped swab
​Wood swab
​Transport swab containing gel
​Feces
​Paraffin blocks
​Breast milk
​Heat inactivated specimens



Interference

​A negative result does not eliminate the possibility of cytomegalovirus (CMV) infection.

This assay is only to be used for patients with a clinical history and symptoms consistent with CMV infection and must be interpreted in the context of the clinical picture.​

Performing Laboratory Information
Performing LocationDay(s) Test Performed
Report Available
Methodology/Instrumentation
​Mayo Clinic Laboratories
​Monday through Sunday
​Same day/1 to 4 days
​Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reference Lab
Mayo Clinic Laboratories
Test Information

Infection with cytomegalovirus (CMV) is a significant cause of morbidity and mortality in transplant recipients and other immunocompromised hosts. Specific neurologic syndromes associated with CMV infection include subacute radiculomyelopathy, peripheral neuropathy, and encephalitis.

Cytomegalovirus-associated central nervous system disease occurs most commonly in immunocompromised patients. Histologic evidence of CMV infections in autopsy brain tissue was identified in 20% to 40% of patients with AIDS. In 2 separate studies, CMV (DNA) was the most common herpesvirus (29/181, 16/49) detected from the cerebrospinal fluid of patients with AIDS. 

Central nervous system infections with CMV can also occur in immunocompetent patients, and infection can result in a mono-like illness. A significant percentage of adults are positive for IgG antibodies against CMV, suggesting prior infection with the virus.


​

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​​
​

Negative

Reference values apply to all ages.

Interpretation

​Detection of cytomegalovirus (CMV) DNA in a specimen supports the clinical diagnosis of infection due to this virus.

Studies indicate that CMV DNA is not detected by polymerase chain reaction in cerebrospinal fluid from patients without central nervous system disease caused by this virus.​​

Outreach CPTs
CPTModifier
(if needed)		Quantity
DescriptionComments
87496​
​​1
​Cytomegalovirus, amplified probe technique
​
Synonyms/Keywords

​​​CMV Detection by Real-Time PCR

Cytomegalovirus, PCR

LightCycler CMV

PCR (Polymerase Chain Reaction)​

Test Components

​Cytomegalovirus PCR

Ordering Applications
Ordering ApplicationDescription
​Cerner
​Cytomegalovirus (CMV), Molecular Detection, PCR, Varies (CMVPV)​

If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements

Specimen TypePreferred Container/TubeAcceptable Container/Tube
Specimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)

​Submit only 1 of the following specimens:​​​​ ​ ​ ​ ​ ​

​
Body Fluid

Sources: Spinal, pleural, peritoneal, ascites, pericardial, amniotic, or ocular .

Collection Instructions: Do not centrifuge.

​Sterile, screwcap, 5-mL aliquot tube
​Sterile container
​0.5 mL
Ocular Fluid, and Spinal Fluid:
0.3 mL

Body Fluid (pleural, peritoneal, ascites, pericardial):
 0.5 mL​
​
​

Upper respiratory tract fluid

Sources: nasopharyngeal aspirate or washing

Collection Instructions: Do not centrifuge.

Note:  for lower respiratory tract specimens,order test code:  CMVLRSO

​Sterile, screwcap, 5-mL aliquot tube
​Sterile container
​1.5 mL
​1.0 mL
​
​

Genital swab

Sources: Cervix, vagina, urethra, anal/rectal, or other genital sources.

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)


​Multimicrobe media (M4-RT) and ESwabs
​​​​
​

Swab

Sources: Dermal, eye, nasal, saliva, or throat.

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)​


​Multimicrobe media (M4-RT) and ESwabs
​​​​
​

Tissue

Sources: Brain, colon, kidney, liver, lung, etc.

Collection Instructions: Submit only fresh tissue in multimicrobe media (M4-RT) or a sterile container with 1 to 2 mL sterile saline


​Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, or M5)
​
​Entire collection
​2 x 2-mm biopsy
​
​Urine

Collection Instructions: Collect a random urine specimen.
​Sterile container
​​1.0 mL​
​0.3 mL​
​

Bone Marrow

Collection Instructions:
Send bone marrow in original tube.  Do not aliquot.​
​EDTA Lavender top (LTT)
​

​​0.5 mL
​
​
Collection Processing

​Specimen source is required.

For plasma specimens order CMVQN / Cytomegalovirus (CMV) DNA Detection and Quantification by Real-Time PCR, Plasma.

For lower respiratory tract specimens (bronchial washing, bronchoalveolar larvage, fluid/washings from lung, sputum, tracheal secretions, tracheal aspirates) order test code:  CMVLR/ Cytomegalovirus (CMV) Molecular Detection, PCR, Lower Respiratory.​​

Specimen Stability Information
Specimen TypeTemperature
Time
​​
Varies
​​
​Refrigerated (preferred)
​7 days
​Frozen
​7 days
Rejection Criteria

​Calcium alginate-tipped swab
​Wood swab
​Transport swab containing gel
​Feces
​Paraffin blocks
​Breast milk
​Heat inactivated specimens



Interference

​A negative result does not eliminate the possibility of cytomegalovirus (CMV) infection.

This assay is only to be used for patients with a clinical history and symptoms consistent with CMV infection and must be interpreted in the context of the clinical picture.​

Useful For

​Rapid qualitative detection of cytomegalovirus (CMV) DNA

This test is not intended for the monitoring of CMV disease progression.

This test should not be used to screen asymptomatic patients.

Test Components

​Cytomegalovirus PCR

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​​
​

Negative

Reference values apply to all ages.

Interpretation

​Detection of cytomegalovirus (CMV) DNA in a specimen supports the clinical diagnosis of infection due to this virus.

Studies indicate that CMV DNA is not detected by polymerase chain reaction in cerebrospinal fluid from patients without central nervous system disease caused by this virus.​​

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test Performed
Report Available
Methodology/Instrumentation
​Mayo Clinic Laboratories
​Monday through Sunday
​Same day/1 to 4 days
​Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)		Quantity
DescriptionComments
87496​
​​1
​Cytomegalovirus, amplified probe technique
​
For most current information refer to the Marshfield Laboratory online reference manual.