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26645 Carbapenem Resistance Genes, Molecular Detection, PCR

Carbapenem Resistance Genes, Molecular Detection, PCR
Test Code: CARBANAT
Test Components

​blaIMP, blaKPC, blaNDM, blaOXA-48 and blaVIM carbapenem-resistance genes.

Useful For

Detection of carbapenem-resistance genes from Enterobacterales, Acinetobacter baumannii, and Pseudomonas aeruginosa isolates by nucleic amplified acid test. 

These bacteria are often resistant to all beta-lactam agents and frequently are co-resistant to multiple classes of other antimicrobial agents, leaving very few treatment options.
Specimen Requirements

Specimen TypePreferred Container/TubeAcceptable Container/Tube
​Pure culture of aerobic bacterium
​Sealed agar plates
​Agar slant (e.g., chocolate, tryptic soy)
Collection Processing Instructions
Submit pure culture of organism on agar plates to keep turn-around-time to a minimum.

Clearly state the name (ID) of the organism submitted for carbapenemase testing. If the identification is not indicated, the organism will be identified, and susceptibility testing will be completed at an additional charge. 

Do not send primary plates or plates with mixed cultures. Multiple organisms submitted will be rejected and facility will be asked to resubmit.

Ensure that plates are properly sealed (e.g., tape or parafilm) to prevent contamination and exposure to laboratory personnel.
Specimen Stability Information
Specimen TypeTemperatureTime​​

​Agar plate or slant
​After incubation, store and transport at room temperature​
​5 days
Rejection Criteria

Isolates submitted that are not Enterobacterales, P. aeruginosa or Acinetobacter baumannii

Mixed isolation plates

Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation

​Marshfield
​Monday through Sunday
​2 days
​​Nucleic Acid Amplification Test
Reference Range Information
Performing LocationReference Range
​Marshfield
​​Not Detected
Interpretation

Not Detected for all five of the carbapenemase targets: No carbapenemase production.

  • Note: A not detected result does not preclude the presence of other carbapenem-resistance mechanisms.
  •  If there is suspect for any other carbapenemase target the microbiology section will forward the isolate to the state lab upon client request with corresponding sensitivity report.

Detected for any of the five targets: Carbapenemase production.

*Carbapenemase producing organisms are reportable conditions in Wisconsin.

Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments

​87150
​​5
​​Culture, typing; identification by nucleic acid probe, per culture or isolate.
Test Components

​blaIMP, blaKPC, blaNDM, blaOXA-48 and blaVIM carbapenem-resistance genes.

Ordering Applications
Ordering ApplicationDescription

​Cerner
​​Carbapenamase Detection by NAT
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements

Specimen TypePreferred Container/TubeAcceptable Container/Tube
​Pure culture of aerobic bacterium
​Sealed agar plates
​Agar slant (e.g., chocolate, tryptic soy)
Collection Processing
Submit pure culture of organism on agar plates to keep turn-around-time to a minimum.

Clearly state the name (ID) of the organism submitted for carbapenemase testing. If the identification is not indicated, the organism will be identified, and susceptibility testing will be completed at an additional charge. 

Do not send primary plates or plates with mixed cultures. Multiple organisms submitted will be rejected and facility will be asked to resubmit.

Ensure that plates are properly sealed (e.g., tape or parafilm) to prevent contamination and exposure to laboratory personnel.
Specimen Stability Information
Specimen TypeTemperatureTime​​

​Agar plate or slant
​After incubation, store and transport at room temperature​
​5 days
Rejection Criteria

Isolates submitted that are not Enterobacterales, P. aeruginosa or Acinetobacter baumannii

Mixed isolation plates

Useful For

Detection of carbapenem-resistance genes from Enterobacterales, Acinetobacter baumannii, and Pseudomonas aeruginosa isolates by nucleic amplified acid test. 

These bacteria are often resistant to all beta-lactam agents and frequently are co-resistant to multiple classes of other antimicrobial agents, leaving very few treatment options.
Test Components

​blaIMP, blaKPC, blaNDM, blaOXA-48 and blaVIM carbapenem-resistance genes.

Reference Range Information
Performing LocationReference Range
​Marshfield
​​Not Detected
Interpretation

Not Detected for all five of the carbapenemase targets: No carbapenemase production.

  • Note: A not detected result does not preclude the presence of other carbapenem-resistance mechanisms.
  •  If there is suspect for any other carbapenemase target the microbiology section will forward the isolate to the state lab upon client request with corresponding sensitivity report.

Detected for any of the five targets: Carbapenemase production.

*Carbapenemase producing organisms are reportable conditions in Wisconsin.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation

​Marshfield
​Monday through Sunday
​2 days
​​Nucleic Acid Amplification Test
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments

​87150
​​5
​​Culture, typing; identification by nucleic acid probe, per culture or isolate.
For most current information refer to the Marshfield Laboratory online reference manual.