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22163 Renal Acidification Test, Urine

Renal Acidification Test, Urine
Test Code: MISC
Synonyms/Keywords
Acidification Test, Renal Acidosis Study, Wong and Davies Test​
Useful For
This test is useful for helping to diagnosis renal tubular acidosis.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Yes​

Urine

Serum/Plasma​

Sage cup - urine, no preservative

Red Top (RTT) or
Lithium-heparin Plasma Separator  (PST)​

 

Lithium or Sodium-heparin Green Top (GTT)​

30-80 mL each specimen

1 mL​

10 mL

 

0.3 mL​

10 mL

 

0.6 mL whole blood​

Collection Processing Instructions
Fasting and post dose (ammonium chloride) - collected hourly for 2-6 hours post dose.  Random urine specimens.  Should be 30-80 mL per each specimen.   HAND CARRY specimens to Lab-Specimen Processing immediately after collection.  Oral test dose of ammonium chloride must be obtained by the nursing unit from Hospital Pharmacy.  Note: The requesting physician will need to order the ammonium chloride test dose (0.1 g/kg in 100-200 mL of flavored water) from the Hospital Pharmacy.
 
A two hour blood sample is also drawn. 
A serum bicarbonate (test code=CO2) or venous blood gas sample for pH and PCO2 should be collected 2 hours after administration of the test dose.​
Specimen Stability Information
Specimen Type Temperature Time
Urine, Random​ Cannot be stored​ Immediately hand deliver to Lab-Specimen Processing​
Rejection Criteria
Reject if conditions of collection not followed
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Schedule in advance.  Monday through Friday​ 4-6 hours​ pH meter/Orion Model 420A​
Reference Range Information
Performing Location Reference Range
Marshfield​ pH of post dose specimens should be < or = 5.5.  If 2 and 3 hour urine pHs are <5.2 test may be discontinued​
​ 2 hour plasma bicarbinate level should be < or = 20 mmol/L for the test to be valid​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​? ​ ​ ​ ​
Synonyms/Keywords
Acidification Test, Renal Acidosis Study, Wong and Davies Test​
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Yes​

Urine

Serum/Plasma​

Sage cup - urine, no preservative

Red Top (RTT) or
Lithium-heparin Plasma Separator  (PST)​

 

Lithium or Sodium-heparin Green Top (GTT)​

30-80 mL each specimen

1 mL​

10 mL

 

0.3 mL​

10 mL

 

0.6 mL whole blood​

Collection Processing
Fasting and post dose (ammonium chloride) - collected hourly for 2-6 hours post dose.  Random urine specimens.  Should be 30-80 mL per each specimen.   HAND CARRY specimens to Lab-Specimen Processing immediately after collection.  Oral test dose of ammonium chloride must be obtained by the nursing unit from Hospital Pharmacy.  Note: The requesting physician will need to order the ammonium chloride test dose (0.1 g/kg in 100-200 mL of flavored water) from the Hospital Pharmacy.
 
A two hour blood sample is also drawn. 
A serum bicarbonate (test code=CO2) or venous blood gas sample for pH and PCO2 should be collected 2 hours after administration of the test dose.​
Specimen Stability Information
Specimen Type Temperature Time
Urine, Random​ Cannot be stored​ Immediately hand deliver to Lab-Specimen Processing​
Rejection Criteria
Reject if conditions of collection not followed
Useful For
This test is useful for helping to diagnosis renal tubular acidosis.​
Reference Range Information
Performing Location Reference Range
Marshfield​ pH of post dose specimens should be < or = 5.5.  If 2 and 3 hour urine pHs are <5.2 test may be discontinued​
​ 2 hour plasma bicarbinate level should be < or = 20 mmol/L for the test to be valid​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Schedule in advance.  Monday through Friday​ 4-6 hours​ pH meter/Orion Model 420A​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​? ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.