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22185 Amylase, Total

Amylase, Total
Test Code: AMY
Synonyms/Keywords
​Amylase - Blood​
Useful For
Total amylase activity in serum and urine is most frequently measured for the diagnosis of pancreatic disorders.  Although amylase is not organ (pancreas) specific, measurement in conjunction with lipase remain the clinician's choice due to fast turnaround time.  Hyperamylasemia is also found in other conditions, including perforated peptic ulcer, ruptured ectopic pregnancy, diabetic ketoacidosis, some renal disease, salivary disease, and macroamylasemia.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Plasma​/Serum Lithium-heparin Plasma Separator Tube (PST), Serum Separator Tube (SST)​ Lithium or Sodium-heparin Green Top (GTT), ​Red Top Tube (RTT)
 1 mL​ 0.5 mL​ 0.5 mL whole blood​
Collection Processing Instructions
Samples collected in a RTT or GTT must be removed from cells within one hour for storage or transport. Samples collected in gel barrier tubes must be removed from the primary tube prior to transporting to Marshfield. Do not send the primary collection tube.​
Specimen Stability Information
Specimen Type Temperature Time
Plasma​/Serum ​ ​ Ambient​ 5 days​
Refrigerate​ 7 days​
Frozen​ 90 days​
Rejection Criteria
Specimens collected with EDTA, oxalate, or sodium citrate preservative.
Performing Laboratory Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Weston​ ​Monday through Sunday​ ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
Eau Claire​ ​Monday through Sunday​ ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
Park Falls
 ​Monday through Sunday​ ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
Ladysmith​ Medical Center ​Monday through Sunday ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
Lakeview Medical Center​ ​Monday through Sunday​ ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
Marshfield​ Monday through Sunday​ Less than 2 hours​ CNPG3 Rate Assay/Beckman AU
​Minocqua ​Monday through Sunday​ ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
​Neillsville​Monday through Sunday​​Less than 2 hours​Biochromatic Rate Technique/Siemens Dimension

​Steven's Point
​Monday through Sunday
Less than 2 hours
​​CNPG3 Rate Assay/Beckman AU​
Reference Range Information
Performing Location Reference Range
All Performing Sites using Dimension 25-100 U/L  ​
​Marshfield
​       0 - 14 days:       <=9​ U/L
15 days - <3 mo:     <=17 U/L
      3 mo - <1 yr:     <=39 U/L
               >=1yr:   18 - 69 U/L
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82150 ​ ​ ​ ​ ​
Synonyms/Keywords
​Amylase - Blood​
Ordering Applications
Ordering Application Description
​Centricity ​Amylase
​Cerner ​Amylase Level (COMMON)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Plasma​/Serum Lithium-heparin Plasma Separator Tube (PST), Serum Separator Tube (SST)​ Lithium or Sodium-heparin Green Top (GTT), ​Red Top Tube (RTT)
 1 mL​ 0.5 mL​ 0.5 mL whole blood​
Collection Processing
Samples collected in a RTT or GTT must be removed from cells within one hour for storage or transport. Samples collected in gel barrier tubes must be removed from the primary tube prior to transporting to Marshfield. Do not send the primary collection tube.​
Specimen Stability Information
Specimen Type Temperature Time
Plasma​/Serum ​ ​ Ambient​ 5 days​
Refrigerate​ 7 days​
Frozen​ 90 days​
Rejection Criteria
Specimens collected with EDTA, oxalate, or sodium citrate preservative.
Useful For
Total amylase activity in serum and urine is most frequently measured for the diagnosis of pancreatic disorders.  Although amylase is not organ (pancreas) specific, measurement in conjunction with lipase remain the clinician's choice due to fast turnaround time.  Hyperamylasemia is also found in other conditions, including perforated peptic ulcer, ruptured ectopic pregnancy, diabetic ketoacidosis, some renal disease, salivary disease, and macroamylasemia.​
Reference Range Information
Performing Location Reference Range
All Performing Sites using Dimension 25-100 U/L  ​
​Marshfield
​       0 - 14 days:       <=9​ U/L
15 days - <3 mo:     <=17 U/L
      3 mo - <1 yr:     <=39 U/L
               >=1yr:   18 - 69 U/L
For more information visit:
Performing Laboratory Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Weston​ ​Monday through Sunday​ ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
Eau Claire​ ​Monday through Sunday​ ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
Park Falls
 ​Monday through Sunday​ ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
Ladysmith​ Medical Center ​Monday through Sunday ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
Lakeview Medical Center​ ​Monday through Sunday​ ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
Marshfield​ Monday through Sunday​ Less than 2 hours​ CNPG3 Rate Assay/Beckman AU
​Minocqua ​Monday through Sunday​ ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
​Neillsville​Monday through Sunday​​Less than 2 hours​Biochromatic Rate Technique/Siemens Dimension

​Steven's Point
​Monday through Sunday
Less than 2 hours
​​CNPG3 Rate Assay/Beckman AU​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82150 ​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.