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22248 Bilirubin, Direct Only

Bilirubin, Direct Only
Test Code: DBIL
Synonyms/Keywords
​DBIL, Conjugated Bilirubin​, Fractionated Bilirubin
Useful For
Direct bilirubin measurements are used in the diagnosis and treatment of liver, hemolytic, hematological, and metabolic disorders, including hepatitis and gall bladder block.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Plasma​/Serum Lithium-heparin Plasma Separator (PST), Serum Separator Tube (SST)​ Lithium or Sodium-heparin Green Top (GTT), Red Top Tube(RTT)
 1 mL​ 0.3 mL​ 0.3 mL serum or plasma from 0.6 mL whole blood
Collection Processing Instructions
Bilirubin is light sensitive.  Protect samples from light.
Samples collected in a SST or RTT must be removed from cells within one hour for storage or transport.
DBIL can only be U-Haved/add-on from a previously collected sample if requested within 24 hours of the original collection, or if the sample has been protected from light.
Specimen Stability Information
Specimen Type Temperature Time
Plasma​/Serum ​ ​ Ambient​ 1 day​
Refrigerate​ 2 days​
Frozen​ 3 months​
Rejection Criteria
Grossly hemolyzed
Interference
Hemolysis falsely decreases DBIL result.​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Chippewa Falls​ ​Monday through Friday ​Less than 2 hours​ ​Bichromatic Endpoint Technique/Siemens Dimension
​Weston
 ​Monday through Sunday​ ​Less than 2 hours​ ​Bichromatic Endpoint Technique/Siemens Dimension
​Eau Claire ​Monday through Sunday​ ​Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/Beckman AU
Park Falls
 Monday through Sunday​​ ​Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/Beckman AU
​Ladysmith Medical Center ​Monday through Sunday ​Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/Beckman AU
​Lakeview Medical Center ​Monday through Sunday​ ​Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/Beckman AU
Marshfield​ Monday through Sunday​ Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/Beckman AU
​Minocqua ​Monday through Sunday​ ​Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/Beckman AU
Neillsville​​Monday through Sunday​​Less than 2 hours​​Bichromatic Endpoint Technique/Siemens Dimension
Stevens Point​ ​Monday through Friday​
 ​Less than 2 hours​ ​BDiazonium Salt/DPD Endpoint Assay/Beckman AU
​Wisconsin Rapids ​Monday through Friday ​Less than 2 hours​ ​Bichromatic Endpoint Technique/Siemens Dimension
Reference Range Information
Performing Location Reference Range
All Performing Sites using Dimension Analyzers​
 0.0 - 0.3 mg/dL​
All Performing Sites using Beckman AU Analyzers
​<=14 days:  <=0.5 mg/dL
>=15 days:  <=0.3 mg/dL
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82248 ​ ​ ​ ​ ​
Synonyms/Keywords
​DBIL, Conjugated Bilirubin​, Fractionated Bilirubin
Ordering Applications
Ordering Application Description
​Centricity ​Bilirubin, Direct
​Cerner ​Bilirubin Direct (COMMON)
​COM ​Bilirubin, Direct
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Plasma​/Serum Lithium-heparin Plasma Separator (PST), Serum Separator Tube (SST)​ Lithium or Sodium-heparin Green Top (GTT), Red Top Tube(RTT)
 1 mL​ 0.3 mL​ 0.3 mL serum or plasma from 0.6 mL whole blood
Collection Processing
Bilirubin is light sensitive.  Protect samples from light.
Samples collected in a SST or RTT must be removed from cells within one hour for storage or transport.
DBIL can only be U-Haved/add-on from a previously collected sample if requested within 24 hours of the original collection, or if the sample has been protected from light.
Specimen Stability Information
Specimen Type Temperature Time
Plasma​/Serum ​ ​ Ambient​ 1 day​
Refrigerate​ 2 days​
Frozen​ 3 months​
Rejection Criteria
Grossly hemolyzed
Interference
Hemolysis falsely decreases DBIL result.​
Useful For
Direct bilirubin measurements are used in the diagnosis and treatment of liver, hemolytic, hematological, and metabolic disorders, including hepatitis and gall bladder block.​
Reference Range Information
Performing Location Reference Range
All Performing Sites using Dimension Analyzers​
 0.0 - 0.3 mg/dL​
All Performing Sites using Beckman AU Analyzers
​<=14 days:  <=0.5 mg/dL
>=15 days:  <=0.3 mg/dL
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Chippewa Falls​ ​Monday through Friday ​Less than 2 hours​ ​Bichromatic Endpoint Technique/Siemens Dimension
​Weston
 ​Monday through Sunday​ ​Less than 2 hours​ ​Bichromatic Endpoint Technique/Siemens Dimension
​Eau Claire ​Monday through Sunday​ ​Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/Beckman AU
Park Falls
 Monday through Sunday​​ ​Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/Beckman AU
​Ladysmith Medical Center ​Monday through Sunday ​Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/Beckman AU
​Lakeview Medical Center ​Monday through Sunday​ ​Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/Beckman AU
Marshfield​ Monday through Sunday​ Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/Beckman AU
​Minocqua ​Monday through Sunday​ ​Less than 2 hours​ Diazonium Salt/DPD Endpoint Assay/Beckman AU
Neillsville​​Monday through Sunday​​Less than 2 hours​​Bichromatic Endpoint Technique/Siemens Dimension
Stevens Point​ ​Monday through Friday​
 ​Less than 2 hours​ ​BDiazonium Salt/DPD Endpoint Assay/Beckman AU
​Wisconsin Rapids ​Monday through Friday ​Less than 2 hours​ ​Bichromatic Endpoint Technique/Siemens Dimension
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82248 ​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.