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22298 Antibody Elution

Antibody Elution
Test Code: DAT
Synonyms/Keywords
​​​Elution Study, Eluate
Useful For
​Used for investigation of positive DAT, and identification of the IgG and/or ABO antibody coating patient cells
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Neonatal Minimum Volume
(no repeat)
No​ Whole blood​ 6 mL EDTA
Pink Top Tube
(PTT)​
One 3 mL EDTA Lavender Top Tube (LTT)
 
Cord blood - EDTA Lavender Top Tube (LTT)​
 3 mL ​ 2.5 mL ​ Two 0.5 mL microcollection tubes​
Specimen Stability Information
Specimen Type Temperature Time
Whole Blood​ Refrigerate​ <72 hours old​
Cord Blood​ Refrigerate​ <72 hours old​
Rejection Criteria
Sample >72 hours old will yield weaker results- OK​
Serum separator tubes ​
Hemolyzed​
Frozen​
Interference

Cells with a positive DAT due to complement normally yield an eluate containing no antibody activity.

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday​ 2-4 hours​ Gamma ELU-KIT II or LUI FREEZE method​
Test Information
Infants >4 months, Pediatrics, and Adults:
Order antibody elution prerequisite DAT to determine if red cells have been coated in vivo with IgG antibody and/or complement.  If positive DAT is from recently transfused patient, an antibody elution will be performed and charged.

Neonates <4 months:
Order antibody elution prerequisite DAT to determine if red cells have been coated with maternal IgG antibody.  If DAT is positive, an antibody elution may be performed and charged based on maternal antibody history.
Reference Range Information
Performing Location Reference Range
​Marshfield No reference ranges are available​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​86860 ​ ​ ​ ​
Synonyms/Keywords
​​​Elution Study, Eluate
Ordering Applications
Ordering Application Description
​Cerner Direct Antiglobulin Test​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Neonatal Minimum Volume
(no repeat)
No​ Whole blood​ 6 mL EDTA
Pink Top Tube
(PTT)​
One 3 mL EDTA Lavender Top Tube (LTT)
 
Cord blood - EDTA Lavender Top Tube (LTT)​
 3 mL ​ 2.5 mL ​ Two 0.5 mL microcollection tubes​
Specimen Stability Information
Specimen Type Temperature Time
Whole Blood​ Refrigerate​ <72 hours old​
Cord Blood​ Refrigerate​ <72 hours old​
Rejection Criteria
Sample >72 hours old will yield weaker results- OK​
Serum separator tubes ​
Hemolyzed​
Frozen​
Interference

Cells with a positive DAT due to complement normally yield an eluate containing no antibody activity.

Useful For
​Used for investigation of positive DAT, and identification of the IgG and/or ABO antibody coating patient cells
Reference Range Information
Performing Location Reference Range
​Marshfield No reference ranges are available​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday​ 2-4 hours​ Gamma ELU-KIT II or LUI FREEZE method​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​86860 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.