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22300 Antibody Screen

Antibody Screen
Test Code: ABSC
Synonyms/Keywords
​​​Indirect Coombs, Indirect Antiglobulin Test, IAT, Atypical antibody screen
Test Components

​If ordering for transfuion of blood products refer to:

Type and Screen (except Neonates)

Neonate Transfusion (infants <4 months old)

Useful For
​Determining the presence of atypical antibodies to red cell antigens in patient plasma or serum.
 
Investigation of immune-mediated hemolytic anemia.
 
Identifying antibody(ies) in prenatal patients to assist in determining the risk for Hemolytic Disease of the Fetus and Newborn (HDFN).
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Whole blood​
6 mL EDTA
Pink Top Tube
(PTT)​
Two 3 mL EDTA Lavender Top Tubes (LTT)
 OR
6 mL Red Top Tube (RTT)​
 6 mL ​
 2.5 mL ​ Two 0.5 mL EDTA microcollection tubes​
Collection Processing Instructions

Invert specimen at least 5-6 times in order to facilitate mixing and prevent clotting.  Do not separate plasma from red cells.

Specimen Stability Information
Specimen Type Temperature Time
​Whole blood/serum Refrigerate​ <10 days old​
Rejection Criteria
Serum separator tubes (SST)
Frozen​
Hemolyzed​
Interference
​Fresh serum, fibrin or particulate matter in the patient specimen may cause anomalous results.  EDTA plasma may minimize these interferences.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Weston​ ​Monday through Sunday​ ​Less than 2 hours ​Solid Phase Red Cell Adherence
Park Falls
 ​Monday through Sunday​ ​Less than 2 hours Column Agglutination Technology
Rice Lake
 ​Monday through Sunday​ ​Less than 2 hours ​Solid Phase Red Cell Adherence
Marshfield​ Monday through Sunday​ Less than 2 hours​ ​Solid Phase Red Cell Adherence
​NeillsvilleMonday through Sunday​​​Less than 2 hours​Solid Phase Red Cell Adherence​
Minocqua​​Monday through Sunday​Less than 2 hours​Solid Phase Red Cell Adherence
​Eau Claire
​Monday through Sunday
​Less than 2 hours
​Soli​d Phase Red Cell Adherence
​Beaver Dam
​Monday through Sunday
​Less than 2 hours
​Gel​
​Ladysmith
​Monday through Sunday
​Less than 2 hours
​Gel
Test Information
​An antibody screen is performed to determine the presence of atypical antibodies.
An Antibody Screen (ABSC) is the appropriate test to be ordered when requesting an antibody identification.
 
Other Testing Performed: Other tests may or may not be performed, at additional charge, depending on the initial test results.  Refer to CPT tables for additional testing codes and description.
Reference Range Information
Performing Location Reference Range
All Performing Sites Negative​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86850 ​ ​ ​ABSC ​
​86870 ​ ​ ​ABID ​​(if needed)
​86886 ​ ​ ​Antibody Titer ​​(if needed)
Synonyms/Keywords
​​​Indirect Coombs, Indirect Antiglobulin Test, IAT, Atypical antibody screen
Test Components

​If ordering for transfuion of blood products refer to:

Type and Screen (except Neonates)

Neonate Transfusion (infants <4 months old)

Ordering Applications
Ordering Application Description
​Centricity ​Antibody Screen
​Cerner ​Antibody Screen
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Whole blood​
6 mL EDTA
Pink Top Tube
(PTT)​
Two 3 mL EDTA Lavender Top Tubes (LTT)
 OR
6 mL Red Top Tube (RTT)​
 6 mL ​
 2.5 mL ​ Two 0.5 mL EDTA microcollection tubes​
Collection Processing

Invert specimen at least 5-6 times in order to facilitate mixing and prevent clotting.  Do not separate plasma from red cells.

Specimen Stability Information
Specimen Type Temperature Time
​Whole blood/serum Refrigerate​ <10 days old​
Rejection Criteria
Serum separator tubes (SST)
Frozen​
Hemolyzed​
Interference
​Fresh serum, fibrin or particulate matter in the patient specimen may cause anomalous results.  EDTA plasma may minimize these interferences.
Useful For
​Determining the presence of atypical antibodies to red cell antigens in patient plasma or serum.
 
Investigation of immune-mediated hemolytic anemia.
 
Identifying antibody(ies) in prenatal patients to assist in determining the risk for Hemolytic Disease of the Fetus and Newborn (HDFN).
Test Components

​If ordering for transfuion of blood products refer to:

Type and Screen (except Neonates)

Neonate Transfusion (infants <4 months old)

Reference Range Information
Performing Location Reference Range
All Performing Sites Negative​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Weston​ ​Monday through Sunday​ ​Less than 2 hours ​Solid Phase Red Cell Adherence
Park Falls
 ​Monday through Sunday​ ​Less than 2 hours Column Agglutination Technology
Rice Lake
 ​Monday through Sunday​ ​Less than 2 hours ​Solid Phase Red Cell Adherence
Marshfield​ Monday through Sunday​ Less than 2 hours​ ​Solid Phase Red Cell Adherence
​NeillsvilleMonday through Sunday​​​Less than 2 hours​Solid Phase Red Cell Adherence​
Minocqua​​Monday through Sunday​Less than 2 hours​Solid Phase Red Cell Adherence
​Eau Claire
​Monday through Sunday
​Less than 2 hours
​Soli​d Phase Red Cell Adherence
​Beaver Dam
​Monday through Sunday
​Less than 2 hours
​Gel​
​Ladysmith
​Monday through Sunday
​Less than 2 hours
​Gel
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86850 ​ ​ ​ABSC ​
​86870 ​ ​ ​ABID ​​(if needed)
​86886 ​ ​ ​Antibody Titer ​​(if needed)
For most current information refer to the Marshfield Laboratory online reference manual.