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22309 Anti-DNA By Crithidia

Anti-DNA By Crithidia
Test Code: NDNA
Synonyms/Keywords
​nDNA (Double stranded) Antibody, NDNA, Anti-native DNA Antibody​
Useful For
A confirmatory test for the evaluation of systemic lupus erythematosus. The Crithidia luciliae nDNA test, detects autoantibodies to nDNA antigens. High titers of anti-DNA, a type of anti-nuclear antibody (ANA), are associated with systemic lupus erythematosus (SLE). Titers of DNA autoantibodies may decrease with successful therapy, and increase in acute recurrence of the disease. DNA-anti-DNA immune complexes play a role in the pathogenesis of SLE through the deposit of the complexes in the kidney and other tissues. For this reason the detection and quantitation of DNA autoantibodies is diagnostically and therapeutically helpful in patients suspected, or known to have SLE.​

See the Mixed Connective Tissue Disease Panel Algorithm for suggested testing strategy. ​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT) or Serum Separator Tube (SST)​ ​ 0.5 mL​ 0.3 mL​ 0.3 mL​
Collection Processing Instructions

​Separate serum from cells within 2 hours of collection.

Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Refrigerate​ <2 days​
Frozen >2 days 
Rejection Criteria
Gross hemolysis
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday, Wednesday, Friday​ 1 day​ Indirect Immunofluorescence (IFA)  ​
Test Information
Substrate is Crithidia lucillae. Positives will be titered.​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative (<1:10) ​
Interpretation

​A positive result supports the diagnosis of systemic lupus erythematosus.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86225 ​ 1​ ​ ​
Synonyms/Keywords
​nDNA (Double stranded) Antibody, NDNA, Anti-native DNA Antibody​
Ordering Applications
Ordering Application Description
​Cerner ​Anti-DNA by Crithidia
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT) or Serum Separator Tube (SST)​ ​ 0.5 mL​ 0.3 mL​ 0.3 mL​
Collection Processing

​Separate serum from cells within 2 hours of collection.

Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Refrigerate​ <2 days​
Frozen >2 days 
Rejection Criteria
Gross hemolysis
Useful For
A confirmatory test for the evaluation of systemic lupus erythematosus. The Crithidia luciliae nDNA test, detects autoantibodies to nDNA antigens. High titers of anti-DNA, a type of anti-nuclear antibody (ANA), are associated with systemic lupus erythematosus (SLE). Titers of DNA autoantibodies may decrease with successful therapy, and increase in acute recurrence of the disease. DNA-anti-DNA immune complexes play a role in the pathogenesis of SLE through the deposit of the complexes in the kidney and other tissues. For this reason the detection and quantitation of DNA autoantibodies is diagnostically and therapeutically helpful in patients suspected, or known to have SLE.​

See the Mixed Connective Tissue Disease Panel Algorithm for suggested testing strategy. ​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative (<1:10) ​
Interpretation

​A positive result supports the diagnosis of systemic lupus erythematosus.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday, Wednesday, Friday​ 1 day​ Indirect Immunofluorescence (IFA)  ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86225 ​ 1​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.