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22319 Anti-Neutrophil Cytoplasmic Antibody (ANCA)

Anti-Neutrophil Cytoplasmic Antibody (ANCA)
Test Code: ANCA
Synonyms/Keywords
MPO, PR3, ANCA, Cytoplasmic Neutrophil antibody​
Test Components
P-ANCA, C-ANCA, Atypical ANCA, and Atypical C-ANCA​
Useful For
Anti-Neutrophil Cytoplasmic Antibodies are autoantibodies directed against cytoplasmic constituents of neutrophils and monocytes. The most common reasons for requesting an ANCA test are to diagnose and monitor inflammatory activity in the primary systemic small vessel vasculitides, namely Wegener’s Granulomatosis, Microscopic Polyangiitis (and its renal-limited form), Pauciimune Segmental Necrotizing Glomerulonephritis, and Churg-Strauss syndrome.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT) or Serum Separator Tube (SST) ​ ​ 0.5 mL​ 0.3 mL​ 0.3 mL​
Collection Processing Instructions

​Separate serum from cells within 2 hours of collection.

Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerate​ <2 days​
Frozen >2 days​
Rejection Criteria
Gross hemolysis
Gross lipemia
Heat treated samples​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day​ Indirect Immunofluorescence (IFA)​
Marshfield​ Monday through Friday​ 1 day​ Muliplex Flow Immunoassay/ Bio-Rad BioPlex 2200 (If needed)​
Test Information
Samples are screened by IFA using ethanol fixed neutrophils. Positive samples will be tested by MPO and PR3 using Multiplex Flow Immunoassay.
For evaluation of autoimmune neutropenia or more rare, febrile non-hemolytic transfusion reactions, refer to Granolycytes Antibodies test performed by Mayo Medical Labs. ​
Reference Range Information
Performing Location Reference Range
Marshfield​ ANCA Negative: <1:20 ​
MPO and PR3  <1.0 Negative​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86036 ​ 1​ ANCA, IFA ​ ​
83516 ​ 2​ MPO, PR3 (if needed) ​ ​
Synonyms/Keywords
MPO, PR3, ANCA, Cytoplasmic Neutrophil antibody​
Test Components
P-ANCA, C-ANCA, Atypical ANCA, and Atypical C-ANCA​
Ordering Applications
Ordering Application Description
​Cerner
Anti-Neutrophil Cytoplasmic Antibody (ANCA)​
​COM

Anti-Neutrophil Cytoplasmic Ab (ANCA)

If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT) or Serum Separator Tube (SST) ​ ​ 0.5 mL​ 0.3 mL​ 0.3 mL​
Collection Processing

​Separate serum from cells within 2 hours of collection.

Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerate​ <2 days​
Frozen >2 days​
Rejection Criteria
Gross hemolysis
Gross lipemia
Heat treated samples​
Useful For
Anti-Neutrophil Cytoplasmic Antibodies are autoantibodies directed against cytoplasmic constituents of neutrophils and monocytes. The most common reasons for requesting an ANCA test are to diagnose and monitor inflammatory activity in the primary systemic small vessel vasculitides, namely Wegener’s Granulomatosis, Microscopic Polyangiitis (and its renal-limited form), Pauciimune Segmental Necrotizing Glomerulonephritis, and Churg-Strauss syndrome.​
Test Components
P-ANCA, C-ANCA, Atypical ANCA, and Atypical C-ANCA​
Reference Range Information
Performing Location Reference Range
Marshfield​ ANCA Negative: <1:20 ​
MPO and PR3  <1.0 Negative​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day​ Indirect Immunofluorescence (IFA)​
Marshfield​ Monday through Friday​ 1 day​ Muliplex Flow Immunoassay/ Bio-Rad BioPlex 2200 (If needed)​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86036 ​ 1​ ANCA, IFA ​ ​
83516 ​ 2​ MPO, PR3 (if needed) ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.