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22330 Anti-Streptolysin O

Anti-Streptolysin O
Test Code: ASO
Synonyms/Keywords
Streptococcal Antibody (ASLO), Streptococcal Ab, ASO​
Useful For
Testing for ADNase-B antibodies is important for confirming an existing or recent streptococcal infection.  The antibody response to streptococcal ADNase-B is stronger, but begins later than the formation of ASLO.  ADNB documents recent Group A Streptococcus infection and is the most consistent of available tests. Unlike ASO, ADNB antibodies are produced in skin infections.​ If ADNase-B testing is desired, please order test code “ADNASSO”.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​
Red Top Tube
(RTT)​
 Serum Separator Tube (SST)​ 0.5 mL 0.4 mL ​ 0.3 mL
Collection Processing Instructions

​Separate serum from cells within 2 hours of collection.

Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerate​ 7 days​
Frozen​ 3 months​
Rejection Criteria


​Plasma Samples
​Highly Lipemic and Hemolyzed Specimens

Performing Laboratory Information
 
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​Monday through Friday​ 6-8 hours​ Turbidimetric method/The Binding Site Optilite analyzer
Reference Range Information
Performing Location Reference Range
Marshfield​
No pediatric reference range
Adult (>18): <200 U/mL
Interpretation
An infection of group A streptococci effects production of specific antibodies against various streptococcal exoenzymes, with two of the most important of being anti-streptolysin O (ASLO) and anti-deoxyribonuclease-B (ADNase B).   Among the assays for antibodies to various streptococcal exoenzymes, the ASLO assay is important as a sensitive parameter, since it has been found to be elevated in about 80-85% of cases.  The sensitivity of antibody testing is increased to 95% when both ADNB and ASO are performed.​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86060​ ​ ​ ​ ​
Synonyms/Keywords
Streptococcal Antibody (ASLO), Streptococcal Ab, ASO​
Ordering Applications
Ordering Application Description
​Cerner ​Anti-Streptolysin O
​COM ​Streptococcal Ab-ASO only
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​
Red Top Tube
(RTT)​
 Serum Separator Tube (SST)​ 0.5 mL 0.4 mL ​ 0.3 mL
Collection Processing

​Separate serum from cells within 2 hours of collection.

Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerate​ 7 days​
Frozen​ 3 months​
Rejection Criteria


​Plasma Samples
​Highly Lipemic and Hemolyzed Specimens

Useful For
Testing for ADNase-B antibodies is important for confirming an existing or recent streptococcal infection.  The antibody response to streptococcal ADNase-B is stronger, but begins later than the formation of ASLO.  ADNB documents recent Group A Streptococcus infection and is the most consistent of available tests. Unlike ASO, ADNB antibodies are produced in skin infections.​ If ADNase-B testing is desired, please order test code “ADNASSO”.
Reference Range Information
Performing Location Reference Range
Marshfield​
No pediatric reference range
Adult (>18): <200 U/mL
Interpretation
An infection of group A streptococci effects production of specific antibodies against various streptococcal exoenzymes, with two of the most important of being anti-streptolysin O (ASLO) and anti-deoxyribonuclease-B (ADNase B).   Among the assays for antibodies to various streptococcal exoenzymes, the ASLO assay is important as a sensitive parameter, since it has been found to be elevated in about 80-85% of cases.  The sensitivity of antibody testing is increased to 95% when both ADNB and ASO are performed.​
For more information visit:
Performing Laboratory Information
 
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​Monday through Friday​ 6-8 hours​ Turbidimetric method/The Binding Site Optilite analyzer
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86060​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.