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22340 Aspartate Amino Transferase

Aspartate Amino Transferase
Test Code: AST
Synonyms/Keywords
SGOT, AST, GOT, Aspartrate Transaminase ​
Useful For
AST is used primarily in the diagnosis of liver and heart muscle disease.  AST is also elevated in 97% of myocardial infarction patients.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No Plasma​/Serum Lithium-heparin Plasma Separator (PST)​, Serum Separator Tube (SST) Lithium or Sodium-heparin Green Top (GTT), Red Top Tube (RTT) 1 mL​ 0.3 mL​ 0.7 mL whole blood​
Collection Processing Instructions
Samples collected in a RTT or GGT must be removed from cells within one hour for storage or transport. Samples collected in gel barrier tubes must be removed from the primary tube prior to transporting to Marshfield. Do not send the primary collection tube. ​
Specimen Stability Information
Specimen Type Temperature Time
Plasma​/Serum ​ ​ Ambient​ 5 days​
Refrigerate​ 7 days​
Frozen​ >7 days to 3 months​
Rejection Criteria
Grossly hemolyzed samples​
Samples collected with sodium citrate, potassium oxalate, or sodium fluoride preservatives
Interference
Hemolysis will falsey increase AST result​.
 
Sulfasalazine may interfere with Dimension AST assays (Regional labs) and cause falsely depressed results.
For patients receiving treatment, venipuncture should occur prior to drug administration to prevent this interference.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Chippewa Falls​ ​Monday through Friday ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
​Weston
 ​Monday through Sunday​ ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
​Eau Claire ​Monday through Sunday​ ​Less than 2 hours​ (IFCC), UV/NADH Rate Assay/Beckman AU​
Park Falls
 ​Monday through Sunday​ ​Less than 2 hours​ ​(IFCC), UV/NADH Rate Assay/Beckman AU​
​Ladysmith Medical Center ​Monday through Sunday ​Less than 2 hours​ (IFCC), UV/NADH Rate Assay/Beckman AU​
Rice Lake
 ​Monday through Sunday​ ​Less than 2 hours​ (IFCC), UV/NADH Rate Assay/Beckman AU​​
Marshfield​
 Monday through Sunday​ Less than 2 hours​ (IFCC), UV/NADH Rate Assay/Beckman AU​
​Minocqua ​Monday through Sunday​ ​Less than 2 hours​ (IFCC), UV/NADH Rate Assay/Beckman AU​
​Neillsville​Monday through Sunday​Less than 2 hours​Biochromatic Rate Technique/Siemens Dimension
​Stevens Point ​Monday through Friday ​Less than 2 hours​ (IFCC), UV/NADH Rate Assay/Beckman AU​
​Wisconsin Rapids ​Monday through Friday ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
Reference Range Information
Performing Location Reference Range
All Performing Sites using Dimension                 0 - 7 days:    20-96 IU/L 
       8 days - 3 years:    16-58 IU/L 
     4 years - 6 years:    10-47 IU/L 
    Male: 7 - 15 years:    10-36 IU/L 
     Male: >=16 years:    15-46 IU/L
Female: 7 - 19 years:    10-36 IU/L 
 Female: >= 20 years:    14-41 IU/L​
Marshfield​ ​Both:      0 - 14 days:      <=134 U/L
          15 days – <1yr:       <=57 U/L
                 1 - <7 yrs:       <=38 U/L
               7 - <12 yrs:       <=32 U/L
Female: 12 - <19 yrs:       <=24 U/L
Male:     12 - <19 yrs:       <=32 U/L
Adult:        >= 19 yrs:      13 - 39 U/L
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
84450 ​ ​ ​ ​ ​
Synonyms/Keywords
SGOT, AST, GOT, Aspartrate Transaminase ​
Ordering Applications
Ordering Application Description
​Centricity

​a. AST (GOT) (Aspartrate Amino Transaminase)

b. GOT (AST, SGOT)

c. SGOT (AST, GOT)

​Cerner ​AST (COMMON)
​COM ​AST (SGOT)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No Plasma​/Serum Lithium-heparin Plasma Separator (PST)​, Serum Separator Tube (SST) Lithium or Sodium-heparin Green Top (GTT), Red Top Tube (RTT) 1 mL​ 0.3 mL​ 0.7 mL whole blood​
Collection Processing
Samples collected in a RTT or GGT must be removed from cells within one hour for storage or transport. Samples collected in gel barrier tubes must be removed from the primary tube prior to transporting to Marshfield. Do not send the primary collection tube. ​
Specimen Stability Information
Specimen Type Temperature Time
Plasma​/Serum ​ ​ Ambient​ 5 days​
Refrigerate​ 7 days​
Frozen​ >7 days to 3 months​
Rejection Criteria
Grossly hemolyzed samples​
Samples collected with sodium citrate, potassium oxalate, or sodium fluoride preservatives
Interference
Hemolysis will falsey increase AST result​.
 
Sulfasalazine may interfere with Dimension AST assays (Regional labs) and cause falsely depressed results.
For patients receiving treatment, venipuncture should occur prior to drug administration to prevent this interference.
Useful For
AST is used primarily in the diagnosis of liver and heart muscle disease.  AST is also elevated in 97% of myocardial infarction patients.​
Reference Range Information
Performing Location Reference Range
All Performing Sites using Dimension                 0 - 7 days:    20-96 IU/L 
       8 days - 3 years:    16-58 IU/L 
     4 years - 6 years:    10-47 IU/L 
    Male: 7 - 15 years:    10-36 IU/L 
     Male: >=16 years:    15-46 IU/L
Female: 7 - 19 years:    10-36 IU/L 
 Female: >= 20 years:    14-41 IU/L​
Marshfield​ ​Both:      0 - 14 days:      <=134 U/L
          15 days – <1yr:       <=57 U/L
                 1 - <7 yrs:       <=38 U/L
               7 - <12 yrs:       <=32 U/L
Female: 12 - <19 yrs:       <=24 U/L
Male:     12 - <19 yrs:       <=32 U/L
Adult:        >= 19 yrs:      13 - 39 U/L
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Chippewa Falls​ ​Monday through Friday ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
​Weston
 ​Monday through Sunday​ ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
​Eau Claire ​Monday through Sunday​ ​Less than 2 hours​ (IFCC), UV/NADH Rate Assay/Beckman AU​
Park Falls
 ​Monday through Sunday​ ​Less than 2 hours​ ​(IFCC), UV/NADH Rate Assay/Beckman AU​
​Ladysmith Medical Center ​Monday through Sunday ​Less than 2 hours​ (IFCC), UV/NADH Rate Assay/Beckman AU​
Rice Lake
 ​Monday through Sunday​ ​Less than 2 hours​ (IFCC), UV/NADH Rate Assay/Beckman AU​​
Marshfield​
 Monday through Sunday​ Less than 2 hours​ (IFCC), UV/NADH Rate Assay/Beckman AU​
​Minocqua ​Monday through Sunday​ ​Less than 2 hours​ (IFCC), UV/NADH Rate Assay/Beckman AU​
​Neillsville​Monday through Sunday​Less than 2 hours​Biochromatic Rate Technique/Siemens Dimension
​Stevens Point ​Monday through Friday ​Less than 2 hours​ (IFCC), UV/NADH Rate Assay/Beckman AU​
​Wisconsin Rapids ​Monday through Friday ​Less than 2 hours​ ​Biochromatic Rate Technique/Siemens Dimension
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
84450 ​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.