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22440 Estradiol

Estradiol
Test Code: ESTRAD
Synonyms/Keywords
​Estrogen, Serum, E2, Beta 17
Specimen Requirements

Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​​​​
 Plasma/Serum​Lithium Heparin Plasma Separator Tube (PST)
Lithium Heparin Green Top Tube (GTT), EDTA Lavender Top Tube (LTT), Serum Separator Tube (SST), Red Top Tube (RTT)
1.0 mL​0.5 mL​0.3 mL​
  • GTT/RTT - physically separate plasma or serum from contact with cells as soon as possible of before transport.  Separate from cells within 30 minutes to minimize uptake of corticosteroids by RBCs​
Collection Processing Instructions
Samples collected in a RTT must be removed from the clot within one hour for storage or transport. Samples collected in gel barrier tubes must be removed from the primary tube prior to transporting to Marshfield. Do not send the primary collection tube.​
Specimen Stability Information

Specimen Type Temperature Time
Plasma/Serum Ambient​ 8 hours​
Refrigerated​ 7 days​
Frozen​ >7 days
Rejection Criteria

​Gross Hemolysis

Gross Icterus

Interference
Human Anti-Murine Antibodies (HAMA) may develop leading to test interference in patients receiving murine based chemotherapy agents or tumor-imaging studies.​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​​ Less than 2 hours​ Beckman Coulter DXI
Reference Range Information
Performing Location Reference Range
Marshfield​ Early follicular:  22.4 – 115
Mid follicular:  25 – 115
Ovulatory Peak:  32.1 – 517
Mid Luteal:  36.5 – 246
Post menopausal:  <=25.1
0 to <1 year:  <=38.2
1 to <12 years (pre-puberty female):  <=16
12 to <19 years (puberty female):  <=196
1 to <12 years (pre-puberty male):  <15
12 to <19 years (puberty male):  <=34.8
Males >19 years:  <15 – 31.5​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82670   ​ ​ ​ ​ ​
Synonyms/Keywords
​Estrogen, Serum, E2, Beta 17
Ordering Applications
Ordering Application Description
​Cerner
 ​Estradiol Level
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements

Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​​​​
 Plasma/Serum​Lithium Heparin Plasma Separator Tube (PST)
Lithium Heparin Green Top Tube (GTT), EDTA Lavender Top Tube (LTT), Serum Separator Tube (SST), Red Top Tube (RTT)
1.0 mL​0.5 mL​0.3 mL​
  • GTT/RTT - physically separate plasma or serum from contact with cells as soon as possible of before transport.  Separate from cells within 30 minutes to minimize uptake of corticosteroids by RBCs​
Collection Processing
Samples collected in a RTT must be removed from the clot within one hour for storage or transport. Samples collected in gel barrier tubes must be removed from the primary tube prior to transporting to Marshfield. Do not send the primary collection tube.​
Specimen Stability Information

Specimen Type Temperature Time
Plasma/Serum Ambient​ 8 hours​
Refrigerated​ 7 days​
Frozen​ >7 days
Rejection Criteria

​Gross Hemolysis

Gross Icterus

Interference
Human Anti-Murine Antibodies (HAMA) may develop leading to test interference in patients receiving murine based chemotherapy agents or tumor-imaging studies.​
Reference Range Information
Performing Location Reference Range
Marshfield​ Early follicular:  22.4 – 115
Mid follicular:  25 – 115
Ovulatory Peak:  32.1 – 517
Mid Luteal:  36.5 – 246
Post menopausal:  <=25.1
0 to <1 year:  <=38.2
1 to <12 years (pre-puberty female):  <=16
12 to <19 years (puberty female):  <=196
1 to <12 years (pre-puberty male):  <15
12 to <19 years (puberty male):  <=34.8
Males >19 years:  <15 – 31.5​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​​ Less than 2 hours​ Beckman Coulter DXI
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82670   ​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.