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22553 D-Dimer, Quantitative

D-Dimer, Quantitative
Test Code: D-DI
Useful For
The D-dimer test is useful for exclusion (but not diagnosis) of acute deep vein thrombosis (DVT) or pulmonary embolism (PE).​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Whole Blood, Plasma​ 3.2% Citrated Blue Top Tube (BTT)​Citrated Blue Top Tube (BTT)​ 1.0 mL Platelet Poor Plasma​ 0.5 mL Platelet Poor Plasma​
Collection Processing Instructions

-Citrated Blue Top Tube (BTT) must be drawn to the fill line.

-Completely invert tube three or four times to mix. Do Not Shake.

-Centrifuge tube at 3000 rpm for 10 minutes or Stat Spin for 3 minutes at 8500 rpm with the brake OFF as soon 

 as possible after collection. 

-If testing cannot be performed within specimen stability transfer plasma (avoiding platelet contamination) to 

 polypropylene tube with plastic pipette for storage within two hours of collection.

-If sending to another testing location: If combined with other coag testing send only one frozen, 

 plasma aliquot (1mL) for all tests, with specimen label.  Also send original Blue Top Tube (BTT) in case sample needs to be checked for clot. 

Specimen Stability Information
Specimen Type Temperature Time
Whole Blood​ Ambient​ 4 Hours​
Refrigerated​ 24 Hours​
​ ​ Plasma​ Ambient​ ​4 hours
Refrigerated​ ​24 hours
Frozen (-20°C)​ 2 Weeks​
Frozen (-70°C)​ 6 Months​
Rejection Criteria
Specimens filled below the fill line
Clotted​
Specimens with abnormal results that have  a Hct >55% that have not had the citrate anticoagulant adjusted​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Diagnostic Treatment Center
Monday through Sunday​ 2-4 hours Immuno​assay/Sysmex CS-2500​
Eau Claire
​Monday through Sunday​ ​2-4 hours ​Immuno​assay/Sysmex CS-2500​
Flambeau Hospital
​Monday through Sunday​ ​2-4 hours ​Immuno​assay/Sysmex CS-2500​
​Ladysmith Medical Center ​Monday through Sunday ​2-4 hours ​Immunoassay/Sysmex CA-620
Lakeview Medical Center
​Monday through Sunday​ ​2-4 hours ​Immuno​assay/Sysmex CS-2500​
Marshfield​​​ ​Monday through Sunday​ ​Less than 2 hours​ ​Immuno​assay/Sysmex CS-2500​
​Merrill ​Monday through Friday ​2-4 hours ​Optical Clot Detection/Sysmex CA-660
​Minocqua ​Monday through Sunday​ ​2-4 hours Immuno​assay/Sysmex CS-2500​
​Neillsville​​Monday through Sunday​​​2-4 hours​Immunoassay/Sysmex CS-2500
​Stevens Point ​Monday through Friday ​2-4 hours ​Optical Clot Detection/Sysmex CA-660
​Wausau ​Monday through Sunday​ ​2-4 hours ​Immuno​assay/Sysmex CS-2500​
​Wisconsin Rapids ​Monday through Friday ​2-4 hours ​Optical Clot Detection/Sysmex CA-660
Reference Range Information
Performing Location Reference Range
Marshfield​ < 190 - 499 ng/mL​
Interpretation
An elevation of D-dimer (>500 ng/mL) indicates activation of coagulation with formation and degradation of cross-linked fibrin. It is a very sensitive, albeit very nonspecific, marker of pulmonary embolism (PE) or deep venous thrombosis (DVT). The main value of D-dimer testing in the setting of possible PE or DVT is its negative rule-out capability. A negative D-dimer result, when the pre-test probability of PE or DVT is low to intermediate, essentially excludes PE and DVT. In healthy adults D-dimer levels increase in a linear fashion after age 50. By optimizing the cutoffs with age, the very high sensitivity of the test can be maintained while providing greater specificity. This allows a greater percentage of older adults to be ruled-out. This allows a simple calculation of AGE x 10 for patients over 50 years old. For example, a 65 year-old patient would now have a cutoff of < 650 ng/mL (i.e. values of 650 ng/mL or less considered negative).
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
85379 ​
Ordering Applications
Ordering Application Description
​Centricity ​D-Dimer
​Cerner D-Dimer​
​COM ​D-Dimer-Blood
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Whole Blood, Plasma​ 3.2% Citrated Blue Top Tube (BTT)​Citrated Blue Top Tube (BTT)​ 1.0 mL Platelet Poor Plasma​ 0.5 mL Platelet Poor Plasma​
Collection Processing

-Citrated Blue Top Tube (BTT) must be drawn to the fill line.

-Completely invert tube three or four times to mix. Do Not Shake.

-Centrifuge tube at 3000 rpm for 10 minutes or Stat Spin for 3 minutes at 8500 rpm with the brake OFF as soon 

 as possible after collection. 

-If testing cannot be performed within specimen stability transfer plasma (avoiding platelet contamination) to 

 polypropylene tube with plastic pipette for storage within two hours of collection.

-If sending to another testing location: If combined with other coag testing send only one frozen, 

 plasma aliquot (1mL) for all tests, with specimen label.  Also send original Blue Top Tube (BTT) in case sample needs to be checked for clot. 

Specimen Stability Information
Specimen Type Temperature Time
Whole Blood​ Ambient​ 4 Hours​
Refrigerated​ 24 Hours​
​ ​ Plasma​ Ambient​ ​4 hours
Refrigerated​ ​24 hours
Frozen (-20°C)​ 2 Weeks​
Frozen (-70°C)​ 6 Months​
Rejection Criteria
Specimens filled below the fill line
Clotted​
Specimens with abnormal results that have  a Hct >55% that have not had the citrate anticoagulant adjusted​
Useful For
The D-dimer test is useful for exclusion (but not diagnosis) of acute deep vein thrombosis (DVT) or pulmonary embolism (PE).​
Reference Range Information
Performing Location Reference Range
Marshfield​ < 190 - 499 ng/mL​
Interpretation
An elevation of D-dimer (>500 ng/mL) indicates activation of coagulation with formation and degradation of cross-linked fibrin. It is a very sensitive, albeit very nonspecific, marker of pulmonary embolism (PE) or deep venous thrombosis (DVT). The main value of D-dimer testing in the setting of possible PE or DVT is its negative rule-out capability. A negative D-dimer result, when the pre-test probability of PE or DVT is low to intermediate, essentially excludes PE and DVT. In healthy adults D-dimer levels increase in a linear fashion after age 50. By optimizing the cutoffs with age, the very high sensitivity of the test can be maintained while providing greater specificity. This allows a greater percentage of older adults to be ruled-out. This allows a simple calculation of AGE x 10 for patients over 50 years old. For example, a 65 year-old patient would now have a cutoff of < 650 ng/mL (i.e. values of 650 ng/mL or less considered negative).
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Diagnostic Treatment Center
Monday through Sunday​ 2-4 hours Immuno​assay/Sysmex CS-2500​
Eau Claire
​Monday through Sunday​ ​2-4 hours ​Immuno​assay/Sysmex CS-2500​
Flambeau Hospital
​Monday through Sunday​ ​2-4 hours ​Immuno​assay/Sysmex CS-2500​
​Ladysmith Medical Center ​Monday through Sunday ​2-4 hours ​Immunoassay/Sysmex CA-620
Lakeview Medical Center
​Monday through Sunday​ ​2-4 hours ​Immuno​assay/Sysmex CS-2500​
Marshfield​​​ ​Monday through Sunday​ ​Less than 2 hours​ ​Immuno​assay/Sysmex CS-2500​
​Merrill ​Monday through Friday ​2-4 hours ​Optical Clot Detection/Sysmex CA-660
​Minocqua ​Monday through Sunday​ ​2-4 hours Immuno​assay/Sysmex CS-2500​
​Neillsville​​Monday through Sunday​​​2-4 hours​Immunoassay/Sysmex CS-2500
​Stevens Point ​Monday through Friday ​2-4 hours ​Optical Clot Detection/Sysmex CA-660
​Wausau ​Monday through Sunday​ ​2-4 hours ​Immuno​assay/Sysmex CS-2500​
​Wisconsin Rapids ​Monday through Friday ​2-4 hours ​Optical Clot Detection/Sysmex CA-660
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
85379 ​
For most current information refer to the Marshfield Laboratory online reference manual.