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22589 Endomysial IgA Antibody

Endomysial IgA Antibody
Test Code: ENDOMYS
Synonyms/Keywords
ENDOMYSIAL AB, Anti-endomysial Antibodies, Sprue​
Useful For
The detection of endomysial antibodies is useful as an aid in the diagnosis of Celiac disease (CD) following a seropositive TTG IgA result, primarily in pediatric patients (ref). CD is an immune-mediated disorder of the intestine, caused by an intolerance to gluten proteins found in wheat, rye, or barley. When individuals with CD ingest gluten, it creates an immune mediated toxic reaction that causes damage to the small intestine. Common symptoms include malabsorption of nutrients, diarrhea, weight loss, anemia, bone disease, and a failure to thrive in infants.
Dermatitis Herpetiformis (DH) is the skin manifestation of CD. It is characterized by blistering, intensely itchy skin. The rash is frequently seen on the face, elbows, knees, and buttocks. ​
JPAN 2012; 54:136-160
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​Serum
Red Top Tube (RTT) or Serum Separator Tube (SST)
​ 0.5 mL​ 0.3 mL​ 0.3 mL​
Collection Processing Instructions
Separate serum from cells within 2 hours of collection.

Do not freeze-thaw serum more than 1 time.​
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Refrigerate​ <2 days​
Frozen >2 days​
Rejection Criteria
Gross hemolysis
Gross lipemia
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday, Wednesday, Friday​ 1 day​ Indirect Immunofluorescence (IFA) ​
Test Information
Endomysial Ab is highly sensitive and specific for celiac disease. Levels decline after the institution of a gluten-free diet. This test is an indirect fluorescent antibody procedure, based on the subjective interpretation of a microscopic pattern using monkey esophagus substrate. ​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative <1:10  ​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86231 ​ ​ ​ ​ ​
Synonyms/Keywords
ENDOMYSIAL AB, Anti-endomysial Antibodies, Sprue​
Ordering Applications
Ordering Application Description
​Centricity ​Endomysial IgA Ab
​Cerner ​Endomysial IgA Ab
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​Serum
Red Top Tube (RTT) or Serum Separator Tube (SST)
​ 0.5 mL​ 0.3 mL​ 0.3 mL​
Collection Processing
Separate serum from cells within 2 hours of collection.

Do not freeze-thaw serum more than 1 time.​
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Refrigerate​ <2 days​
Frozen >2 days​
Rejection Criteria
Gross hemolysis
Gross lipemia
Useful For
The detection of endomysial antibodies is useful as an aid in the diagnosis of Celiac disease (CD) following a seropositive TTG IgA result, primarily in pediatric patients (ref). CD is an immune-mediated disorder of the intestine, caused by an intolerance to gluten proteins found in wheat, rye, or barley. When individuals with CD ingest gluten, it creates an immune mediated toxic reaction that causes damage to the small intestine. Common symptoms include malabsorption of nutrients, diarrhea, weight loss, anemia, bone disease, and a failure to thrive in infants.
Dermatitis Herpetiformis (DH) is the skin manifestation of CD. It is characterized by blistering, intensely itchy skin. The rash is frequently seen on the face, elbows, knees, and buttocks. ​
JPAN 2012; 54:136-160
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative <1:10  ​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday, Wednesday, Friday​ 1 day​ Indirect Immunofluorescence (IFA) ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86231 ​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.