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22594 Human Papillomavirus (HPV)

Human Papillomavirus (HPV)
Test Code: HPV-ONL
Synonyms/Keywords
​HPV, ThinPrep
Useful For

​This test is useful for the detection of Human Papillomavirus (HPV) from the cervical sample associated with a Pap test. 

Please note that this testing can only be performed on a specimen that has been deemed to have adequate cellularity through the cytological screening by Pap test. If Pap testing has been performed on the submitted sample at another lab, please submit a copy of the lab report along with the sample.
If Pap testing has not been performed on the sample, please order HPVSO for the send out test that does not require a Pap. If no Pap test result is available, Marshfield Labs will automatically change order to the send out test.

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​ ThinPrep vial​ Green Label Aptima Tube​ 1.0 mL​ 1.0 mL​ ​
Collection Processing Instructions
Cervical specimens obtained using a spatula / cytobrush collection device and placed in ThinPrep vial containing PreservCyt solution.  
Transport ThinPrep specimen at room temperature overnight.
Specimen Stability Information
Specimen Type Temperature Time
ThinPrep specimen ​ Ambient​ ​30 days
Aptima Specimen Transfer tube​ Ambient​ ​60 days
Rejection Criteria

Insufficient Amount (Less than 1 mL)

Specimen with no accompanying Pap test

Specimen with inadequate cellularity (determined by Pap test) ​

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday through Friday
2-4 days*
 ​Transcription-Mediated Amplification (TMA)
*Testing performed once/day. ​​Specimens will be tested for HPV after adequate cellularity is determined through Pap testing screening. ​ ​ ​
Test Information
​HPV by Transcription-Mediated Amplification (TMA) may be reflexively ordered following a finding of ASCUS on Pap smear, or as clinically indicated. It may also be ordered separately (test code HPV-ONL) if Pap smear was performed on same specimen and cytology report is available indicating adequate cellularity.
Reference Range Information
Performing Location Reference Range
​Marshfield
Reported as Negative, Positive, or Indeterminate for E6/E7 viral messenger RNA (mRNA) from 14 high-risk types (16/18/31/33/35/39/45/51/52/56/58/59/66/68) of human papillomavirus (HPV) in cervical specimens by TMA.​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87624 ​ ​ ​ ​
Synonyms/Keywords
​HPV, ThinPrep
Ordering Applications
Ordering Application Description

​Cerner
​​HPV Only

If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​ ThinPrep vial​ Green Label Aptima Tube​ 1.0 mL​ 1.0 mL​ ​
Collection Processing
Cervical specimens obtained using a spatula / cytobrush collection device and placed in ThinPrep vial containing PreservCyt solution.  
Transport ThinPrep specimen at room temperature overnight.
Specimen Stability Information
Specimen Type Temperature Time
ThinPrep specimen ​ Ambient​ ​30 days
Aptima Specimen Transfer tube​ Ambient​ ​60 days
Rejection Criteria

Insufficient Amount (Less than 1 mL)

Specimen with no accompanying Pap test

Specimen with inadequate cellularity (determined by Pap test) ​

Useful For

​This test is useful for the detection of Human Papillomavirus (HPV) from the cervical sample associated with a Pap test. 

Please note that this testing can only be performed on a specimen that has been deemed to have adequate cellularity through the cytological screening by Pap test. If Pap testing has been performed on the submitted sample at another lab, please submit a copy of the lab report along with the sample.
If Pap testing has not been performed on the sample, please order HPVSO for the send out test that does not require a Pap. If no Pap test result is available, Marshfield Labs will automatically change order to the send out test.

Reference Range Information
Performing Location Reference Range
​Marshfield
Reported as Negative, Positive, or Indeterminate for E6/E7 viral messenger RNA (mRNA) from 14 high-risk types (16/18/31/33/35/39/45/51/52/56/58/59/66/68) of human papillomavirus (HPV) in cervical specimens by TMA.​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday through Friday
2-4 days*
 ​Transcription-Mediated Amplification (TMA)
*Testing performed once/day. ​​Specimens will be tested for HPV after adequate cellularity is determined through Pap testing screening. ​ ​ ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87624 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.