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22614 Immunofixation, Urine-Random

Immunofixation, Urine-Random
Test Code: LIG-CHA
Synonyms/Keywords
Qualitative Urine Light Chains, Urine Protein Electrophoresis, Kappa/Lambda Urine Light Chains-random, Qualitative Bence-Jones Protein​
Useful For
This test is useful in determining if Bence-Jones (free light chain) protein is present in the urine.  Bence-Jones proteins are monoclonal kappa or lambda immunoglobulin light chains, which are not attached to a heavy chain portion of the immunoglobulin molecule.  They may be seen in urine of plasma cell myeloma patients, where a monoclonal heavy chain and light chain, or just a free light chain is being produced.  Bence-Jones protein may occur as a result of protein overload, where high concentrations of serum monoclonal protein appear in the urine.  Free light chains may filter through the glomerulus of the kidney due to their small molecular size, and accumulate in the tubules.  Renal impairment may result from the toxicity of the light chains.
 
Polyclonal light chains may be detected, but are not Bence-Jones proteins.  The polyclonal light chains are generated by increased catabolism of polyclonal light chains in the serum.  They may be found in large quantities in autoimmune diseases such as Lupus Erythothematosis and Rheumatoid Arthritis.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Urine, random​
Sage cup
(no preservative)​
Red Top Tube (RTT) or Sarstedt Urine Aliquot Tube
(no preservative)​
30 mL from a random urine​ 10 mL aliquot​ 6 mL aliquot​
Collection Processing Instructions
Indicate the collection time.​
Specimen Stability Information
Specimen Type Temperature Time
Urine​ Refrigerate​ 3 days​
Rejection Criteria
Specimens collected with a preservative
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 6-8 hours​ Agarose gel electrophoresis and immunofixation/Sebia Hydrasys System​
Reference Range Information
Performing Location Reference Range
Marshfield​ No Bence-Jones protein detected.​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
86335​
Synonyms/Keywords
Qualitative Urine Light Chains, Urine Protein Electrophoresis, Kappa/Lambda Urine Light Chains-random, Qualitative Bence-Jones Protein​
Ordering Applications
Ordering Application Description
​Centricity ​Immunofix, Urine Random
​Cerner ​Immunofixation, Urine Random
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Urine, random​
Sage cup
(no preservative)​
Red Top Tube (RTT) or Sarstedt Urine Aliquot Tube
(no preservative)​
30 mL from a random urine​ 10 mL aliquot​ 6 mL aliquot​
Collection Processing
Indicate the collection time.​
Specimen Stability Information
Specimen Type Temperature Time
Urine​ Refrigerate​ 3 days​
Rejection Criteria
Specimens collected with a preservative
Useful For
This test is useful in determining if Bence-Jones (free light chain) protein is present in the urine.  Bence-Jones proteins are monoclonal kappa or lambda immunoglobulin light chains, which are not attached to a heavy chain portion of the immunoglobulin molecule.  They may be seen in urine of plasma cell myeloma patients, where a monoclonal heavy chain and light chain, or just a free light chain is being produced.  Bence-Jones protein may occur as a result of protein overload, where high concentrations of serum monoclonal protein appear in the urine.  Free light chains may filter through the glomerulus of the kidney due to their small molecular size, and accumulate in the tubules.  Renal impairment may result from the toxicity of the light chains.
 
Polyclonal light chains may be detected, but are not Bence-Jones proteins.  The polyclonal light chains are generated by increased catabolism of polyclonal light chains in the serum.  They may be found in large quantities in autoimmune diseases such as Lupus Erythothematosis and Rheumatoid Arthritis.
Reference Range Information
Performing Location Reference Range
Marshfield​ No Bence-Jones protein detected.​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 6-8 hours​ Agarose gel electrophoresis and immunofixation/Sebia Hydrasys System​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
86335​
For most current information refer to the Marshfield Laboratory online reference manual.