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22657 Gliadin AB (Deamidated) IgG, IgA

Gliadin AB (Deamidated) IgG, IgA
Test Code: GLIADIN
Synonyms/Keywords
​Anti-gliadin Antibodies, Sprue, deamidated gliadin peptide (DGP)​
Useful For
This is a semi-quantitative test for the detection of IgA and IgG antibodies to recombinant, deamidated gliadin-derived peptides in human serum. Gliadin antibodies are found in a high percentage of patients with untreated celiac disease and antibody levels decline after the institution of a gluten-free diet. In comparison with tissue transglutaminase (tTg) and endomysial antibodies, older tests for gliadin antibodies were less specific and could be seen in other intestinal diseases. However, selective in vivo deamidation of gliadin results in enhanced binding by anti-gliadin antibodies and newer assays such as this have higher diagnostic accuracy for celiac disease when compared to standard anti-gliadin assay. In addition, this test can aid in the diagnosis of dermatitis herpetiformis and has utility for monitoring compliance to a gluten-free diet. IgG gliadin is most useful in the 2-10% of patients with celiac disease who are IgA deficient.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​Serum Red Top Tube (RTT) or Serum Separator Tube (SST) ​ ​ 1.0 mL​ 1.0 mL​ 0.5 mL​
Collection Processing Instructions

​Separate serum from cells within 2 hours of collection.

Specimen Stability Information
Specimen Type Temperature Time
Serum ​ Refrigerate​ 7 days​
Frozen > 7 days​
Rejection Criteria
Gross lipemia
Gross hemolysis​
Heat inactivated samples​
​Gross icterus
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day Multiplex Immunoassay
Reference Range Information
Performing Location Reference Range
 Marshfield ​
Negative: < 15 U/mL
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86258​ ​ ​2 Gliadin IgG and Gliadin IgA​ ​
Synonyms/Keywords
​Anti-gliadin Antibodies, Sprue, deamidated gliadin peptide (DGP)​
Ordering Applications
Ordering Application Description
​Centricity ​LAB Gliadin Ab (Deamidated) IgG, IgA
​Cerner ​Gliadin AB (Deamidated) IgG, IgA
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​Serum Red Top Tube (RTT) or Serum Separator Tube (SST) ​ ​ 1.0 mL​ 1.0 mL​ 0.5 mL​
Collection Processing

​Separate serum from cells within 2 hours of collection.

Specimen Stability Information
Specimen Type Temperature Time
Serum ​ Refrigerate​ 7 days​
Frozen > 7 days​
Rejection Criteria
Gross lipemia
Gross hemolysis​
Heat inactivated samples​
​Gross icterus
Useful For
This is a semi-quantitative test for the detection of IgA and IgG antibodies to recombinant, deamidated gliadin-derived peptides in human serum. Gliadin antibodies are found in a high percentage of patients with untreated celiac disease and antibody levels decline after the institution of a gluten-free diet. In comparison with tissue transglutaminase (tTg) and endomysial antibodies, older tests for gliadin antibodies were less specific and could be seen in other intestinal diseases. However, selective in vivo deamidation of gliadin results in enhanced binding by anti-gliadin antibodies and newer assays such as this have higher diagnostic accuracy for celiac disease when compared to standard anti-gliadin assay. In addition, this test can aid in the diagnosis of dermatitis herpetiformis and has utility for monitoring compliance to a gluten-free diet. IgG gliadin is most useful in the 2-10% of patients with celiac disease who are IgA deficient.​
Reference Range Information
Performing Location Reference Range
 Marshfield ​
Negative: < 15 U/mL
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day Multiplex Immunoassay
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86258​ ​ ​2 Gliadin IgG and Gliadin IgA​ ​
For most current information refer to the Marshfield Laboratory online reference manual.