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22663 Glucose, 2 HR Post-Glucola

Glucose, 2 HR Post-Glucola
Test Code: GLU2H
Synonyms/Keywords
​Glucose, 2 hr post-Glucola, i-Stat 2 hr post-Glucola​
Useful For
​The primary use of the oral glucose tolerance test is the diagnosis of diabetes mellitus.  The test measures the ability of the individual to remove an added glucose load from the circulation.  Blood samples are obtained at timed intervals after the ingestion of the test glucose load.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)

Yes

8 Hours​

​
 Plasma​/Serum Lithium-heparin Plasma Separator (PST)​, Serum Separator Tube (SST) Lithium or Sodium-heparin Green Top (GTT), Red Top (RTT), Gray Top (GYTT) 1 mL​ 0.3 mL​ ​
​i-Stat only = Whole Blood ​Lithium-heparin Green Top Tube (GTT) ​ ​1 mL ​200 uL ​
Collection Processing Instructions
​Specimens drawn at 120 minutes only following 75 gram (adult) oral glucose test dose. (The glucola must be consumed within a 5 minute interval; sipping water is permitted.) Serum (RTT) or heparinized plasma is also acceptable when removed from cells within one hour of collection.
 
Patients should be ambulatory and on a high carbohydrate diet for at least 3 days prior to the test. Patient must be fasting for 8 hours prior to start of the tolerance test. Order fasting glucose separately with this test if specified by physician.
 
i-Stat:  Sample must be tested within 10 minutes of collection.
Specimen Stability Information
Specimen Type Temperature Time
Plasma/Serum​ ​ Ambient​ 5 days​
Refrigerate​ 7 days​
​i-Stat Whole Blood ​Ambient ​10 minutes
Rejection Criteria
​​i-Stat testing: clotted samples​
i-Stat testing: anticoaulants other than lithium heparin​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Chippewa Falls​ ​Monday through Friday ​Less than 2 hours​ ​Modified Hexokinase Method/Siemens Dimension
​Colby/Abbotsford ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
​Weston
 ​Monday through Sunday​ ​Less than 2 hours​ ​Modified Hexokinase Method/Siemens Dimension
​Eau Claire ​Monday through Sunday​ ​Less than 2 hours​ ​Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Park Falls
 ​Monday through Sunday​ ​Less than 2 hours​
Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
​Hayward ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
​Ladysmith Medical Center ​​Monday through Sunday ​Less than 2 hours​ ​Hexokinase-UV/NAD Endpoint Assay/ Beckman AU​
​Lakeview Medical Center ​Monday through Sunday​ ​Less than 2 hours​ Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Marshfield​ Monday through Sunday​ Less than 2 hours​ Hexokinas-UV/NAD Endpoint Assay/Beckman AU
​Menomonie ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
​Minocqua Monday through Sunday​​ ​Less than 2 hours​ ​Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Mosinee​ ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
Neillsville​​Monday through Sunday​​Less than 2 hours​​​Modified Hexokinase Method/Siemens Dimension
Phillips​ ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
​Stettin ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
​Stevens Point ​​Monday through Friday ​Less than 2 hours​ ​Hexokinas-UV/NAD Endpoint Assay/Beckman AU​​
​Wisconsin Rapids ​​Monday through Fri​day ​Less than 2 hours​ ​Modified Hexokinase Method/Siemens Dimension
​Wittenberg ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
Reference Range Information
Performing Location Reference Range
All Performing Sites See Interpretation​
Interpretation
​American Diabetes Association Guidelines for the diagnosis of diabetes mellitus in non-pregnant patients (Diabetes Care Jan 2004, S5-S10) are as follows:
Two separate days in which any two of the following events occur:
Fasting glucose >=100 mg/dL or,
Random glucose >=200 mg/dL or,
2 hr glucose following glucose load >=200 mg/dL.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82950 ​ ​ ​ ​ ​
Synonyms/Keywords
​Glucose, 2 hr post-Glucola, i-Stat 2 hr post-Glucola​
Ordering Applications
Ordering Application Description
​Centricity ​Glucose, 2Hr P Gluc
​Cerner ​Glucose 2 Hour Post Glucola
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)

Yes

8 Hours​

​
 Plasma​/Serum Lithium-heparin Plasma Separator (PST)​, Serum Separator Tube (SST) Lithium or Sodium-heparin Green Top (GTT), Red Top (RTT), Gray Top (GYTT) 1 mL​ 0.3 mL​ ​
​i-Stat only = Whole Blood ​Lithium-heparin Green Top Tube (GTT) ​ ​1 mL ​200 uL ​
Collection Processing
​Specimens drawn at 120 minutes only following 75 gram (adult) oral glucose test dose. (The glucola must be consumed within a 5 minute interval; sipping water is permitted.) Serum (RTT) or heparinized plasma is also acceptable when removed from cells within one hour of collection.
 
Patients should be ambulatory and on a high carbohydrate diet for at least 3 days prior to the test. Patient must be fasting for 8 hours prior to start of the tolerance test. Order fasting glucose separately with this test if specified by physician.
 
i-Stat:  Sample must be tested within 10 minutes of collection.
Specimen Stability Information
Specimen Type Temperature Time
Plasma/Serum​ ​ Ambient​ 5 days​
Refrigerate​ 7 days​
​i-Stat Whole Blood ​Ambient ​10 minutes
Rejection Criteria
​​i-Stat testing: clotted samples​
i-Stat testing: anticoaulants other than lithium heparin​
Useful For
​The primary use of the oral glucose tolerance test is the diagnosis of diabetes mellitus.  The test measures the ability of the individual to remove an added glucose load from the circulation.  Blood samples are obtained at timed intervals after the ingestion of the test glucose load.
Reference Range Information
Performing Location Reference Range
All Performing Sites See Interpretation​
Interpretation
​American Diabetes Association Guidelines for the diagnosis of diabetes mellitus in non-pregnant patients (Diabetes Care Jan 2004, S5-S10) are as follows:
Two separate days in which any two of the following events occur:
Fasting glucose >=100 mg/dL or,
Random glucose >=200 mg/dL or,
2 hr glucose following glucose load >=200 mg/dL.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Chippewa Falls​ ​Monday through Friday ​Less than 2 hours​ ​Modified Hexokinase Method/Siemens Dimension
​Colby/Abbotsford ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
​Weston
 ​Monday through Sunday​ ​Less than 2 hours​ ​Modified Hexokinase Method/Siemens Dimension
​Eau Claire ​Monday through Sunday​ ​Less than 2 hours​ ​Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Park Falls
 ​Monday through Sunday​ ​Less than 2 hours​
Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
​Hayward ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
​Ladysmith Medical Center ​​Monday through Sunday ​Less than 2 hours​ ​Hexokinase-UV/NAD Endpoint Assay/ Beckman AU​
​Lakeview Medical Center ​Monday through Sunday​ ​Less than 2 hours​ Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Marshfield​ Monday through Sunday​ Less than 2 hours​ Hexokinas-UV/NAD Endpoint Assay/Beckman AU
​Menomonie ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
​Minocqua Monday through Sunday​​ ​Less than 2 hours​ ​Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Mosinee​ ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
Neillsville​​Monday through Sunday​​Less than 2 hours​​​Modified Hexokinase Method/Siemens Dimension
Phillips​ ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
​Stettin ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
​Stevens Point ​​Monday through Friday ​Less than 2 hours​ ​Hexokinas-UV/NAD Endpoint Assay/Beckman AU​​
​Wisconsin Rapids ​​Monday through Fri​day ​Less than 2 hours​ ​Modified Hexokinase Method/Siemens Dimension
​Wittenberg ​​Monday through Friday ​Less than 2 hours​ ​Amperometrical/i-Stat
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
82950 ​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.