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22677 HCG, Total, Quantitative

HCG, Total, Quantitative
Test Code: HCG-QNT
Synonyms/Keywords
​Human Chorionic Gonadotropin, HCG, Serum, Quantitative, HCG-Pregnancy, Quantitative ​
Useful For

​Used for early detection of pregnancy, monitoring in vitro fertilization patients or for the investigation of suspected ectopic pregnancy or other pregnancy-related complications. This test is not useful for detecting and/or monitoring tumors or gestational trophoblastic disease. It should also not be performed on male patients.​

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum or Plasma​ Serum Separator Tube (SST), Lithium-heparin Plasma Separator Tube (PST)​ Red Top Tube (RTT), Sodium-heparin Green Top Tube (GTT)​ 0.5 mL​ 0.4 mL​ 0.3 mL​
Collection Processing Instructions

Samples must be removed from clot within one hour for storage or transport. Samples collected in gel barrier tubes must be removed from the primary tube prior to transporting to Marshfield. Do not send the primary collection tube.​

For plasma samples only:
 - Sites with centrifuges capable of reaching a speed of 4,400g can centrifuge the collection tube for 3 minutes.
 - Sites with standard centrifugation must centrifuge the collection tube for 10 minutes at 1900g.

Specimen Stability Information
Specimen Type Temperature Time
Serum/Plasma​ ​ Ambient​ 8 hours​
Refrigerated​ 5 days​
Frozen​ >5 days​
Interference
Human Anti-Murine Antibodies (HAMA) may develop leading to test interference in patients receiving murine-based agents for chemotherapy or tumor-imaging studies. Fluorescein will interfere; specimens should not be drawn for 48 hours following fluorescein angiography for diabetic retinopathy.​
 
For testing performed at The Diagnostic & Treatment Center lab (Weston Center): High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay. It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Chippewa Falls​ ​​Monday through Friday ​Less than 2 hours​ ​Two-Step Sandwich Enzyme Immunoassay/Siemens Dimension
​Weston
 ​​Monday through Sunday​ ​Less than 2 hours​ ​​Two-Step Sandwich Enzyme Immunoassay/Siemens Dimension
​Eau Claire ​​Monday through Sunday​ ​Less than 2 hours​ ​​Two-Step immunoenzymatic assay/Beckman access2/DXI
Park Falls
 ​Monday through Sunday​ ​Less than 2 hours​ ​​​​Two-Step immunoenzymatic assay/Beckman access2/DXI
​Ladysmith Medical Center ​​​Monday through Sunday ​Less than 2 hours​ ​​Two-Step immunoenzymatic assay/Beckman access2/DXI​
​Lakeview Medical Center ​Monday through Sunday​ ​Less than 2 hours​ ​​​​Two-Step immunoenzymatic assay/Beckman access2/DXI
Marshfield​ Monday through Sunday​ Less than 2 hours​
Two-site Sandwich Immunoassay using Direct Chemiluminometric Technology/Siemens Centaur ​
Minocqua​ ​​​Monday through Sunday ​Less than 2 hours​ ​​​​Two-Step immunoenzymatic assay/Beckman access2/DXI
Neillsville​​Monday through Sunday​​Less than 2 hours​​​​Two-Step Sandwich Enzyme Immunoassay/Siemens Dimension
Stevens Point​ ​Monday through Friday ​​Less than 2 hours​ ​​​Two-Step immunoenzymatic assay/Beckman access2/DXI​
​Wisconsin Rapids ​Monday through Friday ​Less than 2 hours​ ​Two-Step Sandwich Enzyme Immunoassay/Siemens Dimension
Reference Range Information
Performing Location Reference Range
All Performing Sites
Males: 0-2 mIU/mL​
Females-Non pregnant:  0-6 mIU/mL
Females-Pregnant:  >=25 mIU/mL
Interpretation
Persistent elevations (10-100 mIU/mL) of HCG in females for weeks or months could reflect an interfering substance. HCG assessment in urine or with an alternative serum assay (contact Lab) is recommended in these situations.​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
84702​ ​ ​ ​ ​
Synonyms/Keywords
​Human Chorionic Gonadotropin, HCG, Serum, Quantitative, HCG-Pregnancy, Quantitative ​
Ordering Applications
Ordering Application Description
​Cerner ​Beta Human Chorionic Gonadotropin Quantitative
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum or Plasma​ Serum Separator Tube (SST), Lithium-heparin Plasma Separator Tube (PST)​ Red Top Tube (RTT), Sodium-heparin Green Top Tube (GTT)​ 0.5 mL​ 0.4 mL​ 0.3 mL​
Collection Processing

Samples must be removed from clot within one hour for storage or transport. Samples collected in gel barrier tubes must be removed from the primary tube prior to transporting to Marshfield. Do not send the primary collection tube.​

For plasma samples only:
 - Sites with centrifuges capable of reaching a speed of 4,400g can centrifuge the collection tube for 3 minutes.
 - Sites with standard centrifugation must centrifuge the collection tube for 10 minutes at 1900g.

Specimen Stability Information
Specimen Type Temperature Time
Serum/Plasma​ ​ Ambient​ 8 hours​
Refrigerated​ 5 days​
Frozen​ >5 days​
Interference
Human Anti-Murine Antibodies (HAMA) may develop leading to test interference in patients receiving murine-based agents for chemotherapy or tumor-imaging studies. Fluorescein will interfere; specimens should not be drawn for 48 hours following fluorescein angiography for diabetic retinopathy.​
 
For testing performed at The Diagnostic & Treatment Center lab (Weston Center): High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay. It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Useful For

​Used for early detection of pregnancy, monitoring in vitro fertilization patients or for the investigation of suspected ectopic pregnancy or other pregnancy-related complications. This test is not useful for detecting and/or monitoring tumors or gestational trophoblastic disease. It should also not be performed on male patients.​

Reference Range Information
Performing Location Reference Range
All Performing Sites
Males: 0-2 mIU/mL​
Females-Non pregnant:  0-6 mIU/mL
Females-Pregnant:  >=25 mIU/mL
Interpretation
Persistent elevations (10-100 mIU/mL) of HCG in females for weeks or months could reflect an interfering substance. HCG assessment in urine or with an alternative serum assay (contact Lab) is recommended in these situations.​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Chippewa Falls​ ​​Monday through Friday ​Less than 2 hours​ ​Two-Step Sandwich Enzyme Immunoassay/Siemens Dimension
​Weston
 ​​Monday through Sunday​ ​Less than 2 hours​ ​​Two-Step Sandwich Enzyme Immunoassay/Siemens Dimension
​Eau Claire ​​Monday through Sunday​ ​Less than 2 hours​ ​​Two-Step immunoenzymatic assay/Beckman access2/DXI
Park Falls
 ​Monday through Sunday​ ​Less than 2 hours​ ​​​​Two-Step immunoenzymatic assay/Beckman access2/DXI
​Ladysmith Medical Center ​​​Monday through Sunday ​Less than 2 hours​ ​​Two-Step immunoenzymatic assay/Beckman access2/DXI​
​Lakeview Medical Center ​Monday through Sunday​ ​Less than 2 hours​ ​​​​Two-Step immunoenzymatic assay/Beckman access2/DXI
Marshfield​ Monday through Sunday​ Less than 2 hours​
Two-site Sandwich Immunoassay using Direct Chemiluminometric Technology/Siemens Centaur ​
Minocqua​ ​​​Monday through Sunday ​Less than 2 hours​ ​​​​Two-Step immunoenzymatic assay/Beckman access2/DXI
Neillsville​​Monday through Sunday​​Less than 2 hours​​​​Two-Step Sandwich Enzyme Immunoassay/Siemens Dimension
Stevens Point​ ​Monday through Friday ​​Less than 2 hours​ ​​​Two-Step immunoenzymatic assay/Beckman access2/DXI​
​Wisconsin Rapids ​Monday through Friday ​Less than 2 hours​ ​Two-Step Sandwich Enzyme Immunoassay/Siemens Dimension
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
84702​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.