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22705 Heparin Neutralization - APTT

Heparin Neutralization - APTT
Test Code: APTTH
Synonyms/Keywords
Hepasorb Correction​
Useful For
Hepzyme neutralization of plasma to rule out presence of heparin , typically to rule out possible line contamination with heparin.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​
Whole Blood,
Plasma​
 3.2% Sodium Citrate Blue Top Tube (BTT)​ ​ 1.5 mL plasma​ 1.0 mL plasma​ 0.8 mL plasma​
Collection Processing Instructions
-Citrated Blue Top Tube (BTT) must be drawn to the fill line.
-Completely invert tube three or four times to mix. Do Not Shake.
-Centrifuge tube at 3000 rpm for 10 minutes or Stat Spin for 3 minutes at 8500 rpm with the brake OFF as soon 
 as possible after collection. 
-Perform testing within 4 hours of sample collection or transfer plasma (avoiding platelet contamination) to
 polypropylene tube with plastic pipette for storage within two hours of collection.
Specimen Stability Information
Specimen Type Temperature Time
Whole Blood​ or Plasma Ambient or Refrigerated​ 4 Hours​
Plasma​ Frozen (-18°C)​ 2 Weeks​
Frozen (-70°C)​ 6 Months​
Frozen thawed​ 2 Hours​
Rejection Criteria
Specimens filled below the fill line
Specimens filled above the fill line​
Clotted​
Frozen whole blood​
Known IV fluid or Hickman line fluid contamination​
Specimens with abnormal results that have  a Hct >55% that have not had the citrate anticoagulant adjusted​
​Reject at gross hemolysis
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday​ Less than 2 hours​ Optical Clot Detection/Sysmex CA-1500​
Reference Range Information
Performing Location Reference Range
Marshfield​ 22.4 - 30.7 seconds​
​ See Critical Value List for list of current critical values​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
85525 ​ ​ ​ ​ ​
Synonyms/Keywords
Hepasorb Correction​
Ordering Applications
Ordering Application Description
​Centricity

​a. APTT with Hep Neut

b. Heparin Neutralization - APTT

​Cerner ​APTT with Hep Neut
​COM ​APTT with Heparin Neut
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​
Whole Blood,
Plasma​
 3.2% Sodium Citrate Blue Top Tube (BTT)​ ​ 1.5 mL plasma​ 1.0 mL plasma​ 0.8 mL plasma​
Collection Processing
-Citrated Blue Top Tube (BTT) must be drawn to the fill line.
-Completely invert tube three or four times to mix. Do Not Shake.
-Centrifuge tube at 3000 rpm for 10 minutes or Stat Spin for 3 minutes at 8500 rpm with the brake OFF as soon 
 as possible after collection. 
-Perform testing within 4 hours of sample collection or transfer plasma (avoiding platelet contamination) to
 polypropylene tube with plastic pipette for storage within two hours of collection.
Specimen Stability Information
Specimen Type Temperature Time
Whole Blood​ or Plasma Ambient or Refrigerated​ 4 Hours​
Plasma​ Frozen (-18°C)​ 2 Weeks​
Frozen (-70°C)​ 6 Months​
Frozen thawed​ 2 Hours​
Rejection Criteria
Specimens filled below the fill line
Specimens filled above the fill line​
Clotted​
Frozen whole blood​
Known IV fluid or Hickman line fluid contamination​
Specimens with abnormal results that have  a Hct >55% that have not had the citrate anticoagulant adjusted​
​Reject at gross hemolysis
Useful For
Hepzyme neutralization of plasma to rule out presence of heparin , typically to rule out possible line contamination with heparin.​
Reference Range Information
Performing Location Reference Range
Marshfield​ 22.4 - 30.7 seconds​
​ See Critical Value List for list of current critical values​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday​ Less than 2 hours​ Optical Clot Detection/Sysmex CA-1500​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
85525 ​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.