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22710 Hepatitis B Surface Antibody (Qualitative & Quantitative)

Hepatitis B Surface Antibody (Qualitative & Quantitative)
Test Code: AB
Synonyms/Keywords
HBsAb, Hepatitis Post-vaccine testing, Anti-HBs​
Useful For
To aid in the diagnosis of hepatitis B. Determining the phase of a hepatitis B infection. Determination of immune status.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​Serum Separator Tube (SST)Red Top Tube (RTT)​1 mL​0.5 mL​0.3 mL​
Collection Processing Instructions
Separate serum from the blood within 60 minutes of venipuncture and transport in an aliquot tube. Specimen must be free of particulate matter including fibrin.

Regional/Outreach sites: Please send samples to Marshfield frozen. ​

High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay. It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample. 
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Ambient​ <8 hours​
Refrigerate <48 hours
Frozen​ at -20 °C 6 months
​Frozen at -70 °C ​>6 months
Rejection Criteria
​Grossly hemolyzed
​Grossly icteric
​Specimens containing precipitate
​Cadaver specimens
​Heat-inactivated specimens
​Body fluids other than serum
Interference
Heterophilic antibodies in human serum can react with the immunoglobulins included in the assay components causing interference with immunoassay.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day​ Microparticle Chemiluminometric Immunoassay/Siemens Centaur 
Test Information

Hepatitis B is a viral illness that is transmitted through blood and other body fluids. Both acute and chronic stages occur, with the frequency of progression to chronicity declining with age at time of infection. Chronic hepatitis B often leads to cirrhosis and hepatocellular carcinoma. Hepatitis B is a vaccine-preventable disease.

Limitations:
• The results determined by different assays from different manufacturers can vary due to differences in assay specificities and cannot be used interchangeably.
• Assay performance characteristics have not been established in pregnant women or in populations of immunocompromised or immunosuppressed patients.
• This assay does not differentiate between a vaccine-induced immune response and an immune response induced by infection with HBV. To determine if the anti-HBs response is due to vaccine or HBV infection, a Total anti-Hepatitis B Core assay may be performed.
• This assay is not intended for use in screening blood or plasma donors.
• A positive anti-HBs result does not exclude co-infection by another hepatitis virus.

Reference Range Information
Performing Location Reference Range
Marshfield​

Non-Immune: NON-REACTIVE (<10.0 mIU/mL)

Immune: REACTIVE (>=10.0 mIU/mL)

Interpretation

The presence of hepatitis B surface antibody is consistent with a resolved hepatitis B infection of unknown duration. A result of a single hepatitis B lab study should be interpreted with caution.

Hepatitis B surface antibody can be measured to determine if vaccination is needed or, following a vaccination regimen, to determine if protective immunity has been achieved.

Samples with a concentration of 10 mIU/mL or greater are considered reactive (protective) in accordance with CDC guidelines.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86706​ ​ ​ ​ ​
Synonyms/Keywords
HBsAb, Hepatitis Post-vaccine testing, Anti-HBs​
Ordering Applications
Ordering Application Description
Centricity​

Hepatitis B Surface Ab

and

Hep. B Surf Antibody (HBsAb)​

​Cerner Hepatitis B Surface Antibody​
​Clinical Order Manager ​Hep B Surface Ab (Immune)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​Serum Separator Tube (SST)Red Top Tube (RTT)​1 mL​0.5 mL​0.3 mL​
Collection Processing
Separate serum from the blood within 60 minutes of venipuncture and transport in an aliquot tube. Specimen must be free of particulate matter including fibrin.

Regional/Outreach sites: Please send samples to Marshfield frozen. ​

High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay. It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample. 
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Ambient​ <8 hours​
Refrigerate <48 hours
Frozen​ at -20 °C 6 months
​Frozen at -70 °C ​>6 months
Rejection Criteria
​Grossly hemolyzed
​Grossly icteric
​Specimens containing precipitate
​Cadaver specimens
​Heat-inactivated specimens
​Body fluids other than serum
Interference
Heterophilic antibodies in human serum can react with the immunoglobulins included in the assay components causing interference with immunoassay.
Useful For
To aid in the diagnosis of hepatitis B. Determining the phase of a hepatitis B infection. Determination of immune status.
Reference Range Information
Performing Location Reference Range
Marshfield​

Non-Immune: NON-REACTIVE (<10.0 mIU/mL)

Immune: REACTIVE (>=10.0 mIU/mL)

Interpretation

The presence of hepatitis B surface antibody is consistent with a resolved hepatitis B infection of unknown duration. A result of a single hepatitis B lab study should be interpreted with caution.

Hepatitis B surface antibody can be measured to determine if vaccination is needed or, following a vaccination regimen, to determine if protective immunity has been achieved.

Samples with a concentration of 10 mIU/mL or greater are considered reactive (protective) in accordance with CDC guidelines.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day​ Microparticle Chemiluminometric Immunoassay/Siemens Centaur 
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86706​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.