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22711 Hepatitis B Surface Antigen

Hepatitis B Surface Antigen
Test Code: HAA
Synonyms/Keywords
Hepatitis, Australian Antigen, HBsAg, HAA​
Useful For
To aid in the diagnosis of hepatitis B and determining the phase of a hepatitis B infection.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Serum Separator Tube (SST)​ Red Top Tube (RTT)​ 1 mL​ 0.5 mL​ 0.5 mL​
Collection Processing Instructions
Separate serum from the blood within 60 minutes of venipuncture and transport in an aliquot tube.  Specimen must be free of particulate matter including fibrin.
 
Regional/Outreach sites: Please send samples to Marshfield frozen.​ 
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay. It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Ambient​ <8 hours​
Refrigerate <48 hours
Frozen​ at -20 °C 6 months
​Frozen at -70 °C ​>6 months
Rejection Criteria
​Grossly hemolyzed
​Grossly icteric
​Specimens containing precipitate
​Cadaver specimens
​Heat-inactivated specimens
​Body fluids other than serum
Interference
Heterophilic antibodies in human serum can react with the immunoglobulins included in the assay components causing interference with immunoassay.
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day​  Sandwich Immunoassay using Direct Chemiluminometric Technology/Siemens Centaur ​
Test Information

Hepatitis B is a viral illness that is transmitted through blood and other body fluids. Both acute and chronic stages occur, with the frequency of progression to chronicity declining with age at time of infection. Chronic hepatitis B often leads to cirrhosis and hepatocellular carcinoma. Hepatitis B is a vaccine-preventable disease.

Limitations:
• The results determined by different assays from different manufacturers can vary due to differences in assay specificities and cannot be used interchangeably.
• It is recognized that the current methods for the detection of hepatitis B surface antigen may not detect all potentially infected individuals. A nonreactive test result does not exclude the possibility of exposure to or infection with hepatitis B virus. A nonreactive test result in individuals with prior exposure to hepatitis B may be due to antigen levels below the detection limit of this assay or lack of antigen reactivity to the antibodies in this assay.
• Assay performance characteristics have not been established in populations of immunocompromised or immunosuppressed patients.

Reference Range Information
Performing Location Reference Range
Marshfield​ Reported as "Reactive" or "Non-reactive"​
Interpretation

The presence of hepatitis B surface antigen indicates ongoing acute or chronic hepatitis B infection.

A result of a single hepatitis B lab study should be interpreted with caution.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
87340​ ​ ​ ​ ​
Synonyms/Keywords
Hepatitis, Australian Antigen, HBsAg, HAA​
Ordering Applications
Ordering Application Description
​Centricity

​a. Ag. Hepatitis B Surface

b. Hep. B Surf Antigen (HBsAg)

​Cerner Hepatitis B Surface Antigen​
​Clinical Order Manager ​Hepatitis B Surface Ag
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Serum Separator Tube (SST)​ Red Top Tube (RTT)​ 1 mL​ 0.5 mL​ 0.5 mL​
Collection Processing
Separate serum from the blood within 60 minutes of venipuncture and transport in an aliquot tube.  Specimen must be free of particulate matter including fibrin.
 
Regional/Outreach sites: Please send samples to Marshfield frozen.​ 
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay. It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Ambient​ <8 hours​
Refrigerate <48 hours
Frozen​ at -20 °C 6 months
​Frozen at -70 °C ​>6 months
Rejection Criteria
​Grossly hemolyzed
​Grossly icteric
​Specimens containing precipitate
​Cadaver specimens
​Heat-inactivated specimens
​Body fluids other than serum
Interference
Heterophilic antibodies in human serum can react with the immunoglobulins included in the assay components causing interference with immunoassay.
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay.
Useful For
To aid in the diagnosis of hepatitis B and determining the phase of a hepatitis B infection.
Reference Range Information
Performing Location Reference Range
Marshfield​ Reported as "Reactive" or "Non-reactive"​
Interpretation

The presence of hepatitis B surface antigen indicates ongoing acute or chronic hepatitis B infection.

A result of a single hepatitis B lab study should be interpreted with caution.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 1 day​  Sandwich Immunoassay using Direct Chemiluminometric Technology/Siemens Centaur ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
87340​ ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.