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22719 Kleihauer-Betke Stain

Kleihauer-Betke Stain
Test Code: KL-BET
Synonyms/Keywords
Fetal Red Cell Stain, Acid Elution for Hemoglobin F​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Whole Blood EDTA Lavender Top Tube (LTT)​ 2.0 mL​ ​1.0 mL ​1.0 mL
Collection Processing Instructions

Test performed M-F during normal business hours.  Test performed Off-hours and Weekends for STAT requests only.  Call Clinical Microscopy (800-222-5835, ext.16383) if STAT testing is required. 

* If STAT test request is for Rh immune globulin (RHIG) dosing, a Fetal Bleed Screen (FBSC) should be performed prior to ordering Kleihauer-Betke (K-B) stain.  Please provide patient date/time of delivery and estimated date/time of discharge when calling.

Specimen Stability Information
Specimen Type Temperature Time
​Whole Blood ​Refrigerate ​7 days
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Lakeview Medical Center​ ​Monday through Sunday ​​1 day ​Acid Elution
​Marshfield ​Monday through Friday ​1 day ​Acid Elution
Reference Range Information
Performing Location Reference Range
All Performing Sites ​<0.1 % 
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​85460
Synonyms/Keywords
Fetal Red Cell Stain, Acid Elution for Hemoglobin F​
Ordering Applications
Ordering Application Description
​Centricity ​Kleihauer Betke St.
​Cerner ​Kleihauer-Betke Stain
​COM ​Kleihauer Betke Stain
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Whole Blood EDTA Lavender Top Tube (LTT)​ 2.0 mL​ ​1.0 mL ​1.0 mL
Collection Processing

Test performed M-F during normal business hours.  Test performed Off-hours and Weekends for STAT requests only.  Call Clinical Microscopy (800-222-5835, ext.16383) if STAT testing is required. 

* If STAT test request is for Rh immune globulin (RHIG) dosing, a Fetal Bleed Screen (FBSC) should be performed prior to ordering Kleihauer-Betke (K-B) stain.  Please provide patient date/time of delivery and estimated date/time of discharge when calling.

Specimen Stability Information
Specimen Type Temperature Time
​Whole Blood ​Refrigerate ​7 days
Reference Range Information
Performing Location Reference Range
All Performing Sites ​<0.1 % 
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Lakeview Medical Center​ ​Monday through Sunday ​​1 day ​Acid Elution
​Marshfield ​Monday through Friday ​1 day ​Acid Elution
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​85460
For most current information refer to the Marshfield Laboratory online reference manual.