Specimens collected with 0.5 g sulfamic acid or 6N HCl are preferred. If locally collected, random urine specimen, no preservative is also acceptable.
The specimen should be pH 5.0 or less for analysis, unless the specimen volume is <2mL.
If uric acid is also ordered, no preservative is needed but maintain specimen refrigerated during transport to the receiving facility.
Receiving facility must prepare two 10 mL aliquots, adjusting pH of phosphorus aliquot to 5 or less using 6N hydrochloric acid (also appropriate for calcium, magnesium, oxalate, and citrate) and the pH of a second (uric acid) aliquot to 7.5-8.5 using 20% sodium hydroxide. Alternatively, use Marshfield preservative-containing aliquot tubes which provide automatic pH adjustment.