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Lab Test Reference Manual
Human Reference Manual
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22803
Lab Test Reference Manual
Human Reference Manual
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22803
Iron, Total Iron Binding Capacity and % Saturation, Serum
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Iron, Total Iron Binding Capacity and % Saturation, Serum
Test Code: FETIBC
Overview
Ordering
Specimen
Performing
Clinical/Interpretive
Contacts
Coding
Synonyms/Keywords
Synonyms, Keywords
FETIBC, Transferrin Saturation
Test Components
Test Components
Includes serum iron, serum total iron-binding capacity and a calculated percent iron (transferrin) saturation.
Useful For
Useful For
Alterations in iron and total iron binding capacity (IBCT) levels result from changes in iron intake, absorption, storage, and release mechanisms. Iron is decreased in iron deficiency, acute and chronic infection, and in malignancy. In iron deficiency, transferrin levels are usually increased, giving low percent saturation levels, whereas in the anemia of chronic infection and inflammation, IBCT levels are normal or decreased giving a normal percent saturation. Iron overload with a high percent saturation may occur in hematochromatosis, anemia with increased, but ineffective erythropoiesis (thallassemia major and siderosis), alcoholic liver disease, and dietary or IV overdose.
Specimen Requirements
Specimen Requirements
Fasting Required
Specimen Type
Preferred Container/Tube
Acceptable Container/Tube
Specimen Volume
Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No
Serum/ Plasma
Serum Separator Tube (SST), Lithium-heparin Plasma Separator (PST)
Red Top Tube (RTT),
Lithium or Sodium-heparin Green Top (GTT)
1 mL
0.3 mL
Collection Processing Instructions
Collection Processing
It is recommended that heparinized plasma samples be removed from contact from cells within 2 hours of collection.
Samples collected in a RTT must be removed from clot within one hour for storage or transport.
Specimen Stability Information
Specimen Stability Information
Specimen Type
Temperature
Time
Serum/ Plasma
Refrigerate
2 days
Frozen
1 year
Rejection Criteria
Rejection Criteria
Hemolyzed samples
Interference
Interference
Hemolysis may cause falsely elevated results.
Ingestion of oral contraceptives will elevate iron and/or total iron binding capacity values.
Iron-dextran administration can cause elevations in total serum iron with this methodology.
Performing Laboratory Information
Performing Laboratory Information
Performing Location
Day(s) Test Performed
Analytical Time
Methodology/Instrumentation
Eau Claire
Monday through Sunday
Less than 2 hours
TPTZ Endpoint Assay/Beckman AU
Marshfield
Monday through Sunday
Less than 2 hours
TPTZ Endpoint Assay/Beckman AU
Minocqua
Monday through Sunday
Less than 2 hours
TPTZ Endpoint Assay/Beckman AU
Rice Lake
Monday through Sunday
Less than 2 hours
TPTZ Endpoint Assay/Beckman AU
Test Information
Test Information
Do not order FE or TIBC separately when requesting this test.
Reference Range Information
Reference Range Information
Performing Location
Reference Range
All Performing Sites using
Beckman AU
Iron: 0-14 yr:
24 - 152 µg/dL
Female:
14 - <19 yr:
29 – 191
µg/dL
Male:
14 - <19 yr:
41 – 197
µg/dL
Adult:
42 – 173
µg/dL
Iron Binding Capacity: 260-420 ug/dL
% Saturation:
13 – 50%
All Performing Sites using Dimension
Iron: 40-160 ug/dL
Iron Binding Capacity: 250-450 ug/dL
% Saturation: 14-48%
Interpretation
Interpretations
All calculations are performed by the lab computer and are shown below:
Calculated TIBC (CIBC) in ug/dL = 1.4 x Transferrin (Non-Reporting) in mg/dL
Percent Saturation = 100 x Serum Iron / Calculated TIBC
Outreach CPTs
Outreach CPT Codes
CPT
Modifier
(if needed)
Quantity
Description
Comments
83540
Iron
83550
Iron binding capacity
Synonyms/Keywords
Synonyms, Keywords
FETIBC, Transferrin Saturation
Test Components
Test Components
Includes serum iron, serum total iron-binding capacity and a calculated percent iron (transferrin) saturation.
Ordering Applications
Ordering Applications
Ordering Application
Description
Centricity
Iron, TIBC, & Sat.
Cerner
Iron, TIBC, and Saturation
COM
Iron, TIBC, & Saturation
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Requirements
Fasting Required
Specimen Type
Preferred Container/Tube
Acceptable Container/Tube
Specimen Volume
Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No
Serum/ Plasma
Serum Separator Tube (SST), Lithium-heparin Plasma Separator (PST)
Red Top Tube (RTT),
Lithium or Sodium-heparin Green Top (GTT)
1 mL
0.3 mL
Collection Processing
Collection Processing
It is recommended that heparinized plasma samples be removed from contact from cells within 2 hours of collection.
Samples collected in a RTT must be removed from clot within one hour for storage or transport.
Specimen Stability Information
Specimen Stability Information
Specimen Type
Temperature
Time
Serum/ Plasma
Refrigerate
2 days
Frozen
1 year
Rejection Criteria
Rejection Criteria
Hemolyzed samples
Interference
Interference
Hemolysis may cause falsely elevated results.
Ingestion of oral contraceptives will elevate iron and/or total iron binding capacity values.
Iron-dextran administration can cause elevations in total serum iron with this methodology.
Useful For
Useful For
Alterations in iron and total iron binding capacity (IBCT) levels result from changes in iron intake, absorption, storage, and release mechanisms. Iron is decreased in iron deficiency, acute and chronic infection, and in malignancy. In iron deficiency, transferrin levels are usually increased, giving low percent saturation levels, whereas in the anemia of chronic infection and inflammation, IBCT levels are normal or decreased giving a normal percent saturation. Iron overload with a high percent saturation may occur in hematochromatosis, anemia with increased, but ineffective erythropoiesis (thallassemia major and siderosis), alcoholic liver disease, and dietary or IV overdose.
Test Components
Test Components
Includes serum iron, serum total iron-binding capacity and a calculated percent iron (transferrin) saturation.
Reference Range Information
Reference Range Information
Performing Location
Reference Range
All Performing Sites using
Beckman AU
Iron: 0-14 yr:
24 - 152 µg/dL
Female:
14 - <19 yr:
29 – 191
µg/dL
Male:
14 - <19 yr:
41 – 197
µg/dL
Adult:
42 – 173
µg/dL
Iron Binding Capacity: 260-420 ug/dL
% Saturation:
13 – 50%
All Performing Sites using Dimension
Iron: 40-160 ug/dL
Iron Binding Capacity: 250-450 ug/dL
% Saturation: 14-48%
Interpretation
Interpretations
All calculations are performed by the lab computer and are shown below:
Calculated TIBC (CIBC) in ug/dL = 1.4 x Transferrin (Non-Reporting) in mg/dL
Percent Saturation = 100 x Serum Iron / Calculated TIBC
For more information visit:
http://labtestsonline.org
Performing Laboratory Information
Performing Laboratory Information
Performing Location
Day(s) Test Performed
Analytical Time
Methodology/Instrumentation
Eau Claire
Monday through Sunday
Less than 2 hours
TPTZ Endpoint Assay/Beckman AU
Marshfield
Monday through Sunday
Less than 2 hours
TPTZ Endpoint Assay/Beckman AU
Minocqua
Monday through Sunday
Less than 2 hours
TPTZ Endpoint Assay/Beckman AU
Rice Lake
Monday through Sunday
Less than 2 hours
TPTZ Endpoint Assay/Beckman AU
For billing questions, see Contacts
Outreach CPTs
Outreach CPT Codes
CPT
Modifier
(if needed)
Quantity
Description
Comments
83540
Iron
83550
Iron binding capacity
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For most current information refer to the Marshfield Laboratory online reference manual.