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22850 Rabies Antibody Screen, RFFIT

Rabies Antibody Screen, RFFIT
Test Code: RABIES
Useful For
​Rabies vaccination response.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Serum ​Red Top Tube (RTT) ​Serum Separator Tube (SST)  ​2 mL ​1 mL
​CSF ​Sterile tube/container ​2 mL ​1 mL
Collection Processing Instructions

Collect serum in either RTT or SST, spin down and send serum in plastic transfer tube. 

​Specimen should be drawn 2-3 weeks post-vaccination.

All samples must have an accompanying Rabies Serology by RFFIT method submission form. 

Forms are available on the KSU website.

Specimen Stability Information
Specimen Type​Temperature​Time
​Serum ​​Refrigerated​7-10 days
​Frozen (-20 degrees C)
​CSF ​​Refrigerated​7-10 days
​Frozen (-20 degrees C)
Rejection Criteria
​Plasma
Gross hemolysis
​Gross lipemia
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation

KSU-Rabies Lab

Kansas State University

​Performed Monday and Thursday at 8:00 AM 3-4 weeks
Rapid Fluorescent Foci Inhibition Test 
[serum neutralization (inhibition) test]
Reference Lab
Reference Range Information
Performing Location Reference Range
​KSU-Rabies Lab

​Less than 0.1 IU/mL:  Below detection limit

>/= 0.1 IU/mL:  Above detection limit but below 0.5 IU/mL

>/= 0.5 IU/mL:  Equal to or above 0.5 IU/mL

Interpretation
​In humans, a result of 0.5 IU/mL or higher is considered an acceptable response to rabies vaccination according to the World Health Organization (WHO) guidelines.
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​86382
Ordering Applications
Ordering Application Description
​Centricity ​Rabies Ab.
​Cerner ​Rabies Ab. -Immune Status
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Serum ​Red Top Tube (RTT) ​Serum Separator Tube (SST)  ​2 mL ​1 mL
​CSF ​Sterile tube/container ​2 mL ​1 mL
Collection Processing

Collect serum in either RTT or SST, spin down and send serum in plastic transfer tube. 

​Specimen should be drawn 2-3 weeks post-vaccination.

All samples must have an accompanying Rabies Serology by RFFIT method submission form. 

Forms are available on the KSU website.

Specimen Stability Information
Specimen Type​Temperature​Time
​Serum ​​Refrigerated​7-10 days
​Frozen (-20 degrees C)
​CSF ​​Refrigerated​7-10 days
​Frozen (-20 degrees C)
Rejection Criteria
​Plasma
Gross hemolysis
​Gross lipemia
Useful For
​Rabies vaccination response.
Reference Range Information
Performing Location Reference Range
​KSU-Rabies Lab

​Less than 0.1 IU/mL:  Below detection limit

>/= 0.1 IU/mL:  Above detection limit but below 0.5 IU/mL

>/= 0.5 IU/mL:  Equal to or above 0.5 IU/mL

Interpretation
​In humans, a result of 0.5 IU/mL or higher is considered an acceptable response to rabies vaccination according to the World Health Organization (WHO) guidelines.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation

KSU-Rabies Lab

Kansas State University

​Performed Monday and Thursday at 8:00 AM 3-4 weeks
Rapid Fluorescent Foci Inhibition Test 
[serum neutralization (inhibition) test]
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​86382
For most current information refer to the Marshfield Laboratory online reference manual.