Surgical Pathology, Gross and Microscopic Examination H&E Surgical Pathology, Gross and Microscopic Examination H&E (G&M)
Special Stains – PAS, Masson Trichrome, Jones, and Congo Red
2. Electron Microscopy (EM)
3. Direct Immunofluorescence (DIF) - IgA IF, IgG IF, IgM IF, C3 IF, Fibrinogen IF, C1q IF, Kappa IF, and Lambda IF.
Optimum size of tissue pieces: 2 x 2 x 1 mm
Surgical Pathology, Gross and Micro (Light Microscopy) must be ordered along with Electron Microscopy and Direct Immunofluorescence, all of which will be charged separately.
Marshfield Center: Place fresh specimen in a petri dish with biopsy on gauze moistened with saline and hand delivered to the histology lab.
Other Testing Locations: Samples should be placed into the respective vials of fixative/preservative as soon as possible to limit drying and fixation artifacts. Renal kits can be ordered from Marshfield Labs. Supplies included in these kits are: 1. A vial of 10% neutral buffered formalin for Gross & Micro, Light Microscopy (LM). 2. A vial of Zeus tissue fixative (or Michel’s) for Immunofluorescence (IF), If Zeus is not available, call the lab for instructions on possible specimen freezing and shipping. The fresh biopsy should be placed in a vial of Zeus transport media (or Michel’s) immediately upon excision. Be certain that the tissue is immersed in the media and not stuck to the edge of the vial or snap cap before tightly replacing the cap. 3. A vial of 2.5% buffered glutaraldehyde for electron microscopy (EM). Specimens must be 1.0 mm or less in one dimension to insure proper penetration of the fixative before autolysis occurs. Tissue must be placed into fixative within one-half hour of removal from patient. Do not freeze any of these specimens at any time. Label specimens with patient name, date of birth, and fixative type. Submit completed histology requisition containing patient history, clinical and relevant laboratory test information, requesting physician, collection date, and tissue source, must accompany the specimen. Electronic orders are acceptable, but a hard copy must be sent with the specimen.
1. EM - Handling that will interfere with results include: a. Tissue placed in formalin fixative b. Frozen or yellowed glutaraldehyde fixative that has degraded and will not preserve the tissue adequately. Please order fresh fixative and keep refrigerated until ready to use. c. Tissues removed from the patient more than one hour before being placed in the fixative d. Samples that have become dried out before being placed in the fixative d. Samples that have become dried out before being placed in the fixative 2. Immunofluorescence – Tissues transported in expired, cloudy, crystalline, or frozen Zeus transport media or tissue that has been frozen and thawed may interfere with results.
This test was developed and its performance characteristics determined by Marshfield Labs. It has not been cleared or approved by the US Food and Drug Administration. This test is used for clinical purposes. It should not be regarded as investigational or for research.