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22864 SCL70 Antibodies

SCL70 Antibodies
Test Code: SCL70
Synonyms/Keywords
Scleroderma Ab, Topoisomerase I, ENA​
Useful For
A confirmatory test for the evaluation of patients with signs and symptoms of scleroderma and other CTD. SCL70 is also known as "DNA-topoisomerase 1". SCL70 antibodies are frequently presented as a speckled pattern by ANA IFA.

See the Mixed Connective Tissue Disease Panel Algorithm for suggested testing strategy. ​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT) or Serum Separator Tube (SST) ​ ​ 1.0 mL​ 0.4 mL​ 0.3 mL​
Collection Processing Instructions

​Separate serum from cells within 2 hours of collection.

Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerate​ 7 days​
Frozen > 7 days​
Rejection Criteria
Gross lipemia
Gross hemolysis
Heat inactivated samples​​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday  ​ 1 day​ Multiplex Flow Immunoassay/Bio-Rad BioPlex 2200​
Test Information
The test system includes a recombinant Scl-70 antigen.​
Reference Range Information
Performing Location Reference Range
Marshfield ​ Negative: <1.0​
Interpretation

​A positive result supports the diagnosis of scleroderma.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86235 ​ ​ ​ ​
Synonyms/Keywords
Scleroderma Ab, Topoisomerase I, ENA​
Ordering Applications
Ordering Application Description
​Cerner ​Scl 70 Antibodies​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT) or Serum Separator Tube (SST) ​ ​ 1.0 mL​ 0.4 mL​ 0.3 mL​
Collection Processing

​Separate serum from cells within 2 hours of collection.

Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerate​ 7 days​
Frozen > 7 days​
Rejection Criteria
Gross lipemia
Gross hemolysis
Heat inactivated samples​​
Useful For
A confirmatory test for the evaluation of patients with signs and symptoms of scleroderma and other CTD. SCL70 is also known as "DNA-topoisomerase 1". SCL70 antibodies are frequently presented as a speckled pattern by ANA IFA.

See the Mixed Connective Tissue Disease Panel Algorithm for suggested testing strategy. ​
Reference Range Information
Performing Location Reference Range
Marshfield ​ Negative: <1.0​
Interpretation

​A positive result supports the diagnosis of scleroderma.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday  ​ 1 day​ Multiplex Flow Immunoassay/Bio-Rad BioPlex 2200​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
86235 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.