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22985 Protein S Antigen (Free), No Coumadin

Protein S Antigen (Free), No Coumadin
Test Code: PROTSI
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
*Platelet Poor Plasma 3.2% Citrated Blue Top Tube (BTT)​ ​ 0.75 mL​ 0.75 mL​ 0.5 mL​
Collection Processing Instructions
One 0.75 mL citrated Blue Top Tube (BTT) plasma aliquot.
If more than one coagulation test is ordered, a separate aliquot is needed for each test.
-Collect in Citrated Blue Top Tube (BTT)
-Citrate anticoagulant must be adjusted for HCT >55%
-Tube must be at least 90% full
-Invert completely 3-4 times (without shaking) to mix
*See Instructions for: Preparation of Platelet Poor Plasma
Specimen Stability Information
Specimen Type Temperature Time
Plasma​ ​Frozen ​6 months
Rejection Criteria
Clotted or Hemolyzed
​Contamination with IV or Hickman Line fluids
​Specimens that thaw during storage or transport
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday and Friday ​Less than 2 hours Immuno-turbidimetric /IL ACL TOP 550​
Test Information

Free Protein S Antigen will be performed.

Do not order if the patient has received Coumadin for the past two weeks. If patient is on a stable dose of Coumadin (2 weeks), see Protein S Antigen, On Coumadin test (PROT-SM).

Free Protein S Antigen is the initial test in the algorithm for the diagnosis of Protein S deficiency. This assay has better accuracy and less potential interference that Protein S Activity.

When indicated, Protein S Activity testing will be initiated by a clinical pathologist who has reviewed the case.

 

See Clinical Practice Guidelines for more information
Reference Range Information
Performing Location Reference Range
​Marshfield
Free: 55-146 %
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​85306 ​ ​ ​Protein S, Free ​
Ordering Applications
Ordering Application Description
​Centricity ​Protein S Ag-No Coumadin
​Cerner ​Protein S Total-NO Coumadin
​COM ​Protein S Ag-No Coumadin
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
*Platelet Poor Plasma 3.2% Citrated Blue Top Tube (BTT)​ ​ 0.75 mL​ 0.75 mL​ 0.5 mL​
Collection Processing
One 0.75 mL citrated Blue Top Tube (BTT) plasma aliquot.
If more than one coagulation test is ordered, a separate aliquot is needed for each test.
-Collect in Citrated Blue Top Tube (BTT)
-Citrate anticoagulant must be adjusted for HCT >55%
-Tube must be at least 90% full
-Invert completely 3-4 times (without shaking) to mix
*See Instructions for: Preparation of Platelet Poor Plasma
Specimen Stability Information
Specimen Type Temperature Time
Plasma​ ​Frozen ​6 months
Rejection Criteria
Clotted or Hemolyzed
​Contamination with IV or Hickman Line fluids
​Specimens that thaw during storage or transport
Reference Range Information
Performing Location Reference Range
​Marshfield
Free: 55-146 %
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday and Friday ​Less than 2 hours Immuno-turbidimetric /IL ACL TOP 550​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​85306 ​ ​ ​Protein S, Free ​
For most current information refer to the Marshfield Laboratory online reference manual.