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23058 Human Papillomavirus (HPV) High/Low Risk, In Situ Hybridization (HPVHL)

Human Papillomavirus (HPV) High/Low Risk, In Situ Hybridization (HPVHL)
Test Code: HPVHLSO
Synonyms/Keywords
​HPV (Human Papillomavirus), Papillomavirus, GPHPVHLISH, ISHPV611ISH, HPV E6/E7, ISRNAHPVE6E7ISH, ISHPVE6E7IISH, ISNEGHPVE6E7ISH, Surgical Pathology HPV DNA ISH (HPVHL), HPV High/Low Risk DNA ISH (HPVHL) 
Useful For
Detection of human papillomavirus for both low-risk (6, 11) and high-risk (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82) genotypes
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Formalin-fixed, paraffin-embedded tissue block ​ ​ ​Entire block ​
​6 unstained glass, positively charged slides with 5 (+ or - 1)-microns formalin-fixed, paraffin-embedded tissue ​ ​ ​6 slides ​ ​
Collection Processing Instructions
A pathology/diagnostic report and a brief history are required.
Specimen Stability Information
Specimen Type Temperature
​Tissue Slides Ambient
Formalin-fixed, paraffin-embedded tissue block ​ Ambient (preferred)
​Refrigerate
Rejection Criteria
Wet/Frozen Tissue
​Cytology Smears
​Nonformalin fixed tissue
​Nonparaffin embedded tissue
​Noncharged slides
​ProbeOn slides
Interference

​Age of a cut paraffin section can affect staining quality.  Stability thresholds vary widely among published literature.  Best practice is for paraffin sections to be cut within 6 weeks.

Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Friday ​5 to 7 days In Situ Hybridization (ISH)
Reference Lab
Mayo Clinic Laboratories
Test Information

Human papillomavirus (HPV) infections with low-risk genotypes (6, 11) can cause benign hyperplasia such as condylomas and papillomas. Persistent infections with high-risk genotypes (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82) are associated with cervical, vaginal, vulvar, and head and neck malignancies. Patients with HPV-related oropharyngeal squamous cell carcinoma (OPSCC) have shown better disease-specific survival and overall survival when compared to HPV-negative cases of OPSCC.

Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​Results are reported as positive or negative for types 6 and 11 (low risk), and 16, 18, 26, 31, 33, 35, 39, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82 (high risk).
Interpretation
This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
88365 ​ ​1 Primary ​
​88364 ​ ​1 ​If additional ISH ​
Synonyms/Keywords
​HPV (Human Papillomavirus), Papillomavirus, GPHPVHLISH, ISHPV611ISH, HPV E6/E7, ISRNAHPVE6E7ISH, ISHPVE6E7IISH, ISNEGHPVE6E7ISH, Surgical Pathology HPV DNA ISH (HPVHL), HPV High/Low Risk DNA ISH (HPVHL) 
Ordering Applications
Ordering Application Description​
​COM​HPV High/Low Risk DNA ISH (HPVHL)
​Cerner​HPV High/Low Risk DNA ISH (HPVHL)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Formalin-fixed, paraffin-embedded tissue block ​ ​ ​Entire block ​
​6 unstained glass, positively charged slides with 5 (+ or - 1)-microns formalin-fixed, paraffin-embedded tissue ​ ​ ​6 slides ​ ​
Collection Processing
A pathology/diagnostic report and a brief history are required.
Specimen Stability Information
Specimen Type Temperature
​Tissue Slides Ambient
Formalin-fixed, paraffin-embedded tissue block ​ Ambient (preferred)
​Refrigerate
Rejection Criteria
Wet/Frozen Tissue
​Cytology Smears
​Nonformalin fixed tissue
​Nonparaffin embedded tissue
​Noncharged slides
​ProbeOn slides
Interference

​Age of a cut paraffin section can affect staining quality.  Stability thresholds vary widely among published literature.  Best practice is for paraffin sections to be cut within 6 weeks.

Useful For
Detection of human papillomavirus for both low-risk (6, 11) and high-risk (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82) genotypes
Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​Results are reported as positive or negative for types 6 and 11 (low risk), and 16, 18, 26, 31, 33, 35, 39, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82 (high risk).
Interpretation
This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Friday ​5 to 7 days In Situ Hybridization (ISH)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
88365 ​ ​1 Primary ​
​88364 ​ ​1 ​If additional ISH ​
For most current information refer to the Marshfield Laboratory online reference manual.