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23062 Surgical Pathology, Special Stains, Group II

Surgical Pathology, Special Stains, Group II
Test Code: HMISC
Synonyms/Keywords
​​Special Stains, Histology
Useful For
Special stains can be used to help aide in diagnosis. ​
Specimen Requirements
 
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​TissueTissue, Paraffin Embedded Block, or Unstained Air-dried Slides, Fixed in 10% Neutral Buffered Formalin.  If sending unstained slides, call for additional information. ​ ​ ​ ​
Collection Processing Instructions
Submit completed Histology requisition form with patient history and tissue source information.
 
This test is surgical pathology Group II stains, all others (Ex. Iron, Trichrome). Stains included in this group are as follows: Alcian Blue, Alcian Blue PAS, Alkaline Congo Red for Amyloid Digestion by Congo Red, Bielschowsky, Colloidal Iron, Elastic/Masson, Fontana Masson-Argentaffin, Gomori's One-step Trichrome, Gomori's Iron, Gordon and Sweet Reticulum, Grimelius Technique for Argyrophil, Jones, Luxol Fast Blue-PAS Stain, Masson Trichrome, Mayer's Mucicamine, Melanin Bleach, Oil Red (Dezna), PAS with Diatase, PAS Stain, PTAH for CNS 1-day, Rhodamine (same day), Schmorl's Method for Reducing Substances, Toluidine Blue for Mucin, Verhoeff's Elastic Tissue Stain, Victoria Blue.
Specimen Stability Information

​

​Specimen Type​Temperature​Time
​Tissue​Room Temperature/Ambient​Fixed in 10% Neutral Buffered Formalin Total Time 6-72 Hours
​Paraffin Embedded Tissue/Cell Blocks​Room Temperature/Ambient​Indefinite
​Unstained Slides, Air-dried - Call Histology for type, number, thickness, and additional information on slides required.​Room Temperature/Ambient​Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent.  Best practice is for paraffin sections to be cut fresh.

 

Performing Laboratory Information
 
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday - Friday​3 days ​
Reference Range Information
Performing Location Reference Range
​Marshfield ​By report
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​88313 ​ ​ ​ ​
Synonyms/Keywords
​​Special Stains, Histology
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
 
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​TissueTissue, Paraffin Embedded Block, or Unstained Air-dried Slides, Fixed in 10% Neutral Buffered Formalin.  If sending unstained slides, call for additional information. ​ ​ ​ ​
Collection Processing
Submit completed Histology requisition form with patient history and tissue source information.
 
This test is surgical pathology Group II stains, all others (Ex. Iron, Trichrome). Stains included in this group are as follows: Alcian Blue, Alcian Blue PAS, Alkaline Congo Red for Amyloid Digestion by Congo Red, Bielschowsky, Colloidal Iron, Elastic/Masson, Fontana Masson-Argentaffin, Gomori's One-step Trichrome, Gomori's Iron, Gordon and Sweet Reticulum, Grimelius Technique for Argyrophil, Jones, Luxol Fast Blue-PAS Stain, Masson Trichrome, Mayer's Mucicamine, Melanin Bleach, Oil Red (Dezna), PAS with Diatase, PAS Stain, PTAH for CNS 1-day, Rhodamine (same day), Schmorl's Method for Reducing Substances, Toluidine Blue for Mucin, Verhoeff's Elastic Tissue Stain, Victoria Blue.
Specimen Stability Information

​

​Specimen Type​Temperature​Time
​Tissue​Room Temperature/Ambient​Fixed in 10% Neutral Buffered Formalin Total Time 6-72 Hours
​Paraffin Embedded Tissue/Cell Blocks​Room Temperature/Ambient​Indefinite
​Unstained Slides, Air-dried - Call Histology for type, number, thickness, and additional information on slides required.​Room Temperature/Ambient​Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent.  Best practice is for paraffin sections to be cut fresh.

 

Useful For
Special stains can be used to help aide in diagnosis. ​
Reference Range Information
Performing Location Reference Range
​Marshfield ​By report
For more information visit:
Performing Laboratory Information
 
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday - Friday​3 days ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​88313 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.