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23099 Herpes Simplex Virus (HSV) 1/2 by Rapid PCR

Herpes Simplex Virus (HSV) 1/2 by Rapid PCR
Test Code: HS12PCR
Synonyms/Keywords
​HSV by PCR, VAG
Useful For

Useful for detection and differentiation of HSV I and II DNA in CSF, vesicular fluid, and cellular material from base of lesion – (skin, mucosal, urogenital).  This test should not be used for any other source, including ocular.  For ocular specimens order MISC for HSVPV to Mayo Clinic Laboratories.

Please note this test should not be used to screen asymptomatic patients or as neonatal screening for HSV exposure during delivery.  In suspected cases of congenital herpes, order MISC for VHSV culture to Mayo Clinic Laboratories.​

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​CSF ​Sterile, leak-proof vial ​ ​1.0 mL ​0.5 mL ​0.25 mL
​No ​Swab
Universal Transport Media (UTM)
 BD UVT, Remel M4, Remel M4RT, Remel M5, Remel M6
 1 swab​ ​ ​
Collection Processing Instructions
CSF: spinal fluid (CSF) in a sterile, leak-proof container.

Swabs: Vesicular fluid and cellular material from the base of lesion (skin, mucosal, urogenital). Specimens from early stage vesicular lesions rather than ulcerative or crusted lesions should be obtained. Place swabs in transport medium and break off swab at breakpoint of shaft.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Specimen Stability Information
Specimen Type Temperature Time
CSF​ Refrigerate​ ​7 days
​Frozen ​30 days
​Swab ​Refrigerate ​7 days
Rejection Criteria

Collected on Wood-Shafted, Cotton, or Calcium Alginate Swabs
Urine and Body Fluids (with the exception of CSF)​
​Solid Tissue Samples
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Lesions: ​Monday - Friday
CSF: Monday - Sunday		 ​6-8 hours* ​PCR/Hybridization Probe
Test Information

HS12PCR is a real-time polymerase chain reaction (PCR) based qualitative in vitro diagnostic test for the direct amplification and detection of HSV-1 and HSV-2 from unprocessed cutaneous and mucocutaneous lesion swab specimens and CSF.Negative results do not preclude HSV-1 or HSV-2 infection and should not be used as the sole basis for treatment or other patient​

Reference Range Information
Performing Location Reference Range
​Marshfield ​Negative
Interpretation
​Negative: No Herpes Simplex Virus DNA detected by Rapid PCR Positives: Herpes simplex type 1 DNA detected or Herpes simplex type 2 DNA detected
- All positive HS12PCR (CSF) results are called to the provider.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87529 ​ ​2​
 ​ ​
Synonyms/Keywords
​HSV by PCR, VAG
Ordering Applications
Ordering Application Description
​Cerner Herpes Simplex Virus (HSV) 1/2 by Rapid PCR​

​

If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​CSF ​Sterile, leak-proof vial ​ ​1.0 mL ​0.5 mL ​0.25 mL
​No ​Swab
Universal Transport Media (UTM)
 BD UVT, Remel M4, Remel M4RT, Remel M5, Remel M6
 1 swab​ ​ ​
Collection Processing
CSF: spinal fluid (CSF) in a sterile, leak-proof container.

Swabs: Vesicular fluid and cellular material from the base of lesion (skin, mucosal, urogenital). Specimens from early stage vesicular lesions rather than ulcerative or crusted lesions should be obtained. Place swabs in transport medium and break off swab at breakpoint of shaft.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Specimen Stability Information
Specimen Type Temperature Time
CSF​ Refrigerate​ ​7 days
​Frozen ​30 days
​Swab ​Refrigerate ​7 days
Rejection Criteria

Collected on Wood-Shafted, Cotton, or Calcium Alginate Swabs
Urine and Body Fluids (with the exception of CSF)​
​Solid Tissue Samples
Useful For

Useful for detection and differentiation of HSV I and II DNA in CSF, vesicular fluid, and cellular material from base of lesion – (skin, mucosal, urogenital).  This test should not be used for any other source, including ocular.  For ocular specimens order MISC for HSVPV to Mayo Clinic Laboratories.

Please note this test should not be used to screen asymptomatic patients or as neonatal screening for HSV exposure during delivery.  In suspected cases of congenital herpes, order MISC for VHSV culture to Mayo Clinic Laboratories.​

Reference Range Information
Performing Location Reference Range
​Marshfield ​Negative
Interpretation
​Negative: No Herpes Simplex Virus DNA detected by Rapid PCR Positives: Herpes simplex type 1 DNA detected or Herpes simplex type 2 DNA detected
- All positive HS12PCR (CSF) results are called to the provider.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Lesions: ​Monday - Friday
CSF: Monday - Sunday		 ​6-8 hours* ​PCR/Hybridization Probe
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87529 ​ ​2​
 ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.