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23182 Apolipoprotein E Genotyping (APOEG)

Apolipoprotein E Genotyping (APOEG)
Test Code: MISC
Synonyms/Keywords
APOE, Dislipidemia, Dysbetalipoproteinemia, Dyslipidemia, Dyslipoproteinemia, Frederickson Type III, Hyperlipidemia Type III, Type III hyperlipoproteinemia, Alzheimer disease, Dementia
Useful For

Determining the specific apolipoprotein E (APOE) genotypes in patients with type III hyperlipoproteinemia

APOE genotyping has been used to assess susceptibility for Alzheimer disease. However, the use of APOE analysis for predictive testing for Alzheimer disease is not currently recommended by the American College of Medical Genetics due to limited clinical utility and poor predictive value.

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Whole blood​ ​EDTA Lavender Top Tube (LTT) ​ACD Yellow Top Tube (YTT) ​3 mL
Collection Processing Instructions
Specimen preferred to arrive within 96 hours of draw.
 
Patient Preparation: A previous bone marrow transplant from an allogenic donor will interfere with testing. Call 800-533-1710 for instructions for testing patients who have received a bone marrow transplant.
 
Special Instructions and forms:
Specimen Stability Information
Specimen Type Temperature Time
Whole blood​ ​ ​ Ambient (preferred)​ 96 hours​
​Frozen ​96 hours
​Refrigerated ​96 hours
Rejection Criteria

​All specimens will be evaluated at Mayo Clinic Laboratories for test suitability. 

Interference

This assay will not detect all of the mutations that cause type III hyperlipoproteinemia. Therefore, the absence of a detectable mutation does not rule out the possibility that an individual is a carrier of or affected with this disease.

This assay cannot predict or rule out the development of Alzheimer disease in an individual.

Test results should be interpreted in the context of clinical findings, family history, and other laboratory data. Errors in our interpretation of results may occur if information given is inaccurate or incomplete.

Rare polymorphisms exist that could lead to false-negative or false-positive results. If results obtained do not match the clinical findings, additional testing should be considered.

In rare cases, DNA alterations of undetermined significance may be identified.

  This assay does not identify all of the less common apolipoprotein E alleles. Thus, an individual who appears to be homozygous for e2, e3, or e4 may carry 1 of the rare alleles that cannot be detected by this assay.

Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
Mayo Clinic Laboratories​​ Friday ​ 5 to 10 days​
Polymerase Chain Reaction (PCR), Including Restriction Digest
(PCR is utilized pursuant to a license agreement with Roche Diagnostic Systems, Inc.)​
Reference Lab
Interpretation
An interpretive report will be provided​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​81401
Synonyms/Keywords
APOE, Dislipidemia, Dysbetalipoproteinemia, Dyslipidemia, Dyslipoproteinemia, Frederickson Type III, Hyperlipidemia Type III, Type III hyperlipoproteinemia, Alzheimer disease, Dementia
Ordering Applications
Ordering Application Description
​COM ​Misc Test
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Whole blood​ ​EDTA Lavender Top Tube (LTT) ​ACD Yellow Top Tube (YTT) ​3 mL
Collection Processing
Specimen preferred to arrive within 96 hours of draw.
 
Patient Preparation: A previous bone marrow transplant from an allogenic donor will interfere with testing. Call 800-533-1710 for instructions for testing patients who have received a bone marrow transplant.
 
Special Instructions and forms:
Specimen Stability Information
Specimen Type Temperature Time
Whole blood​ ​ ​ Ambient (preferred)​ 96 hours​
​Frozen ​96 hours
​Refrigerated ​96 hours
Rejection Criteria

​All specimens will be evaluated at Mayo Clinic Laboratories for test suitability. 

Interference

This assay will not detect all of the mutations that cause type III hyperlipoproteinemia. Therefore, the absence of a detectable mutation does not rule out the possibility that an individual is a carrier of or affected with this disease.

This assay cannot predict or rule out the development of Alzheimer disease in an individual.

Test results should be interpreted in the context of clinical findings, family history, and other laboratory data. Errors in our interpretation of results may occur if information given is inaccurate or incomplete.

Rare polymorphisms exist that could lead to false-negative or false-positive results. If results obtained do not match the clinical findings, additional testing should be considered.

In rare cases, DNA alterations of undetermined significance may be identified.

  This assay does not identify all of the less common apolipoprotein E alleles. Thus, an individual who appears to be homozygous for e2, e3, or e4 may carry 1 of the rare alleles that cannot be detected by this assay.

Useful For

Determining the specific apolipoprotein E (APOE) genotypes in patients with type III hyperlipoproteinemia

APOE genotyping has been used to assess susceptibility for Alzheimer disease. However, the use of APOE analysis for predictive testing for Alzheimer disease is not currently recommended by the American College of Medical Genetics due to limited clinical utility and poor predictive value.

Interpretation
An interpretive report will be provided​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
Mayo Clinic Laboratories​​ Friday ​ 5 to 10 days​
Polymerase Chain Reaction (PCR), Including Restriction Digest
(PCR is utilized pursuant to a license agreement with Roche Diagnostic Systems, Inc.)​
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​81401
For most current information refer to the Marshfield Laboratory online reference manual.