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24757 Vitamin B7 (FBIOT)

Vitamin B7 (FBIOT)
Test Code: VB7SO
Synonyms/Keywords
​ Biotin, H (Biotin), Vitamin B7
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Serum​ ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​2.0 mL ​1.0 mL
Collection Processing Instructions
Draw blood in a plain, no additive red-top tube(s) or serum gel
tube(s).  Spin down and send 2 mL serum frozen in amber vial to protect from light.
Specimen Stability Information
Specimen Type Temperature​Time​Special Container
​Serum ​​
​Frozen (preferred)
​14 days​LIGHT PROTECTED

​Refrigerated
​7 days
​LIGHT PROTECTED
Rejection Criteria
Specimens other than serum
​Hemolysis
Gross
​Lipemia
Gross
​Other​Not light protected
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available
Methodology/Instrumentation
​Mayo Clinic Laboratories who forwards to BioAgilytix Diagnostics Varies, 2 days per week
4-12 days
Microbiological Assay
Reference Lab
Test Information

Biotin, vitamin B7, or vitamin H, is a water-soluble vitamin. The vitamin plays a role in the transferring of carbon dioxide in the metabolism of fat, carbohydrate and protein by functioning as an enzyme cofactor. It is involved in multiple biochemical reactions including niacin metabolism, amino acid degradation, and the formation of purine, which is an integral part of nucleic acids. It interacts with histone by the action of biotinyl-transferase. Sometimes the vitamin is used in weight reduction programs. It may be prescribed as a supplement for diabetic patients due to its role in carbohydrate metabolism. Biotin is commonly found in vitamin B complex and many food sources, such as milk, yeast, egg yolk, cereal, and mushrooms. The reference daily intake for vitamin B7 is 300 micrograms. Deficiency in the vitamin may result in seborrheic dermatitis, alopecia, myalgia, hyperesthesia, and conjunctivitis. Disorders of biotin metabolism can be acquired or congenital. Biotinidase and holocarboxylase synthethase deficiency are the two better known forms of disorders. The lack of biotin-dependent pyruvate carboxylase, propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, and acetyl-CoA carboxylase can lead to the life-threatening disorder of multiple carboxylase deficiency. Treatment involves a daily dose of approximately 10 mg biotin/day. Irreversible mental or neurological abnormalities may result from delayed clinical intervention.

Reference Range Information
Pediatric Normal < 12 yrs: 100.0 – 2460.2 pg/mL
Adults Normal > or = 12 yrs: 221.0 – 3004.0 pg/mL
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​84591
Synonyms/Keywords
​ Biotin, H (Biotin), Vitamin B7
Ordering Applications
Ordering Application Description
​COM
Vitamin B7 (Biotin) (FBIOT)​
​Cerner ​Vitamin B7 (FBIOT)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Serum​ ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​2.0 mL ​1.0 mL
Collection Processing
Draw blood in a plain, no additive red-top tube(s) or serum gel
tube(s).  Spin down and send 2 mL serum frozen in amber vial to protect from light.
Specimen Stability Information
Specimen Type Temperature​Time​Special Container
​Serum ​​
​Frozen (preferred)
​14 days​LIGHT PROTECTED

​Refrigerated
​7 days
​LIGHT PROTECTED
Rejection Criteria
Specimens other than serum
​Hemolysis
Gross
​Lipemia
Gross
​Other​Not light protected
Reference Range Information
Pediatric Normal < 12 yrs: 100.0 – 2460.2 pg/mL
Adults Normal > or = 12 yrs: 221.0 – 3004.0 pg/mL
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available
Methodology/Instrumentation
​Mayo Clinic Laboratories who forwards to BioAgilytix Diagnostics Varies, 2 days per week
4-12 days
Microbiological Assay
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​84591
For most current information refer to the Marshfield Laboratory online reference manual.