Yes (12-14 hours)
Fasting-overnight (12-14 hours) (infants-draw prior to next feeding).
Serum: Draw blood in a plain, red-top tube(s). Spin down and send 2 mL of serum.
Testing of nonfasting specimens or the use of vitamin K1 supplementation can result in elevated serum vitamin K1 concentrations.
Vitamin K1 or phylloquinone is part of a group of similar fat-soluble vitamins in which the 2-methyl-1,4-naphthoquinone ring is common. Phylloquinone is found in high amounts in leafy green vegetables and some fruits (avocado, kiwi). It is a required cofactor involved in the gamma-carboxylation of glutamate residues of several proteins. Most notably, the inactive forms of the coagulation factors prothrombin (factor II), factors VII, IX, and X, and protein S and protein C are converted to their active forms by the transformation of glutamate residues to gamma-carboxyglutamic acid (Gla). Other proteins such as those involved in bone metabolism, cell growth, and apoptosis also undergo this Gla transformation. Measurement of vitamin K1 (phylloquinone) in fasting serum is a strong indicator of dietary intake and status.