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24908 Ascorbic Acid (VITC)

Ascorbic Acid (VITC)
Test Code: VITCSO
Synonyms/Keywords
Vitamin C
Useful For
Identifying vitamin C deficiency
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Yes Heparinized Plasma Sodium or Lithium ​Heparin Green Top Tube (GTT) 1 mL​ ​0.5 mL
Collection Processing Instructions
1. Fasting-overnight (12-14 hours) (infants-draw prior to next feeding). Water can be taken as needed.
2. Immediately place specimen on wet ice. Maintain specimen on wet ice and process within 4 hours of collection.
3. Centrifuge at 4 degrees C, aliquot plasma into Amber vial to protect from light and freeze immediately.
Specimen Stability Information
Specimen Type Temperature Time​Special Container
​Heparinized Plasma ​Frozen ​14 days​Light Protected
Rejection Criteria

Gross hemolysis, specimens not protected from light

Interference

Testing of nonfasting specimens or the use of vitamin supplementation can result in elevated plasma vitamin concentrations. Reference values were established in patients who were fasting.

After consuming vitamin C, plasma values rapidly rise within 1 to 2 hours and reach peak concentration within 3 to 6 hours after ingestion.

Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories Monday through Friday​ ​3 to 5 days
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)​
Reference Lab
Test Information

Vitamin C, also known as L-ascorbic acid or simply ascorbic acid, is a water-soluble vitamin that is naturally present in some foods, added to others, and available as a dietary supplement. Humans, unlike most animals, are unable to synthesize vitamin C endogenously, so it is an essential dietary component. Vitamin C is required for the enzymatic amidation of neuropeptides, production of adrenal cortical steroid hormones, promotion of the conversion of tropocollagen to collagen, and metabolism of tyrosine and folate. It also plays a role in lipid and vitamin metabolism and is a powerful reducing agent or antioxidant. Specific actions include activation of detoxifying enzymes in the liver; antioxidation, interception and destruction of free radicals; preservation and restoration of the antioxidant potential of vitamin E; and blockage of the formation of carcinogenic nitrosamines. In addition, vitamin C appears to function in a variety of other metabolic processes in which its role has not been well characterized.

Prolonged deficiency of vitamin C leads to the development of scurvy, a disease characterized by an inability to form adequate intercellular substance in connective tissues. This results in the formation of swollen, ulcerative lesions in the gums, mouth, and other tissues that are structurally weakened. Early symptoms may include weakness, easy fatigue and listlessness, as well as shortness of breath, and aching joints, bones, and muscles.

The need for vitamin C can be increased by the use of aspirin, oral contraceptives, tetracycline, and a variety of other medications. Psychological stress and advancing age also tend to increase the need for vitamin C. Among older adults, lack of fresh fruit and vegetables often adds vitamin C depletion to the inherently increased need, with development of near-scurvy status.

Reference Range Information

​0.4-2.0 mg/dL

Interpretation

Values below 0.2 mg/dL indicate significant deficiency.

Values greater than or equal to 0.2 mg/dL and less than 0.4 mg/dL are consistent with a moderate risk of deficiency due to inadequate tissue stores.

Values of 0.4 to 2.0 mg/dL indicate adequate supply.

The actual level at which vitamin C is excessive has not been defined. Values above 3.0 mg/dL are suggestive of excess intake. Whether vitamin C in excess is indeed toxic continues to be uncertain. However, limited observations suggest that this condition may induce uricosuria and, in individuals with glucose-6-phosphate dehydrogenase deficiency, may induce increased red blood cell fragility.

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​82180
Synonyms/Keywords
Vitamin C
Ordering Applications
Ordering Application Description
​Cerner Ascorbic Acid (VITC)

If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Yes Heparinized Plasma Sodium or Lithium ​Heparin Green Top Tube (GTT) 1 mL​ ​0.5 mL
Collection Processing
1. Fasting-overnight (12-14 hours) (infants-draw prior to next feeding). Water can be taken as needed.
2. Immediately place specimen on wet ice. Maintain specimen on wet ice and process within 4 hours of collection.
3. Centrifuge at 4 degrees C, aliquot plasma into Amber vial to protect from light and freeze immediately.
Specimen Stability Information
Specimen Type Temperature Time​Special Container
​Heparinized Plasma ​Frozen ​14 days​Light Protected
Rejection Criteria

Gross hemolysis, specimens not protected from light

Interference

Testing of nonfasting specimens or the use of vitamin supplementation can result in elevated plasma vitamin concentrations. Reference values were established in patients who were fasting.

After consuming vitamin C, plasma values rapidly rise within 1 to 2 hours and reach peak concentration within 3 to 6 hours after ingestion.

Useful For
Identifying vitamin C deficiency
Reference Range Information

​0.4-2.0 mg/dL

Interpretation

Values below 0.2 mg/dL indicate significant deficiency.

Values greater than or equal to 0.2 mg/dL and less than 0.4 mg/dL are consistent with a moderate risk of deficiency due to inadequate tissue stores.

Values of 0.4 to 2.0 mg/dL indicate adequate supply.

The actual level at which vitamin C is excessive has not been defined. Values above 3.0 mg/dL are suggestive of excess intake. Whether vitamin C in excess is indeed toxic continues to be uncertain. However, limited observations suggest that this condition may induce uricosuria and, in individuals with glucose-6-phosphate dehydrogenase deficiency, may induce increased red blood cell fragility.

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Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories Monday through Friday​ ​3 to 5 days
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)​
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​82180
For most current information refer to the Marshfield Laboratory online reference manual.