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24933 Riboflavin (Vitamin B2), Plasma (VITB2)

Riboflavin (Vitamin B2), Plasma (VITB2)
Test Code: VITB2SO
Synonyms/Keywords
​Ref Lab Code: 61637, Riboflavin, Vitamin B2
Useful For
Evaluation of persons who present the signs of ariboflavinosis​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Yes​ ​Plasma Heparin Sodium ​Heparin Green Top Tube (GTT) or Lithium Heparin Green Top Tube (GTT) ​Plasma gel separator tubes (PST) 2.0 mL​ 0.5 mL​
Collection Processing Instructions
1. Fasting-overnight (12-14 hours) (infants-draw prior to next feeding).
2. Place heparin collection tube on ice, keep covered to protect from light.
3. Centrifuge within 2 hours of collection and aliquot to amber vial.
4. Ship specimen in amber vial to protect from light.
Specimen Stability Information
Specimen Type Temperature Time​Special Container
​Plasma Heparin Refrigerated (preferred) 28 days​LIGHT PROTECTED
Frozen ​28 days​LIGHT PROTECTED
​Ambient​72 Hours​LIGHT PROTECTED
Rejection Criteria
Hemolysis Mild OK
​Lipemia ​Gross Reject
​Icterus ​Mild OK
​Other EDTA or serum or specimen NOT protected from light
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories ​Monday, Wednesday, Friday 2 days ​
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)​
Reference Lab
Test Information

​Dietary deficiency of riboflavin (ariboflavinosis) is characterized by sore throat, cheilosis (lesions on the lips), angular stomatitis (lesions on the angles of the mouth), glossitis (fissured and magenta-colored tongue), corneal vascularization, dyssebacia (red, scaly, greasy patches on the nose, eyelids, scrotum, and labia), and normocytic, normochromic anemia. Severe riboflavin deficiency may affect the conversion of vitamin B(6) to its coenzyme, as well as conversion of tryptophan to niacin.

Riboflavin has a low level of toxicity and no case of riboflavin toxicity in humans has been reported. The limited absorptivity of riboflavin and its ready excretion in the urine normally preclude a health problem due to increased intake of riboflavin.


Testing of nonfasting specimens or the use of dietary vitamin B2 supplementation can result in elevated plasma vitamin B2 concentrations.

Reference Range Information
Performing Location Reference Range
​Mayo Medical Laboratories ​1-19 mcg/mL
Interpretation
Low concentrations in the blood plasma are indicative of nutritional deficiency. Concentrations <1 mcg/L are considered significantly diminished. Marginally low levels probably represent nutritional deficiency that should be corrected. ​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​84252
Synonyms/Keywords
​Ref Lab Code: 61637, Riboflavin, Vitamin B2
Ordering Applications
Ordering Application Description
​Centricity ​Riboflavin (VITB2)
​Cerner ​Vitamin B2 (80230)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Yes​ ​Plasma Heparin Sodium ​Heparin Green Top Tube (GTT) or Lithium Heparin Green Top Tube (GTT) ​Plasma gel separator tubes (PST) 2.0 mL​ 0.5 mL​
Collection Processing
1. Fasting-overnight (12-14 hours) (infants-draw prior to next feeding).
2. Place heparin collection tube on ice, keep covered to protect from light.
3. Centrifuge within 2 hours of collection and aliquot to amber vial.
4. Ship specimen in amber vial to protect from light.
Specimen Stability Information
Specimen Type Temperature Time​Special Container
​Plasma Heparin Refrigerated (preferred) 28 days​LIGHT PROTECTED
Frozen ​28 days​LIGHT PROTECTED
​Ambient​72 Hours​LIGHT PROTECTED
Rejection Criteria
Hemolysis Mild OK
​Lipemia ​Gross Reject
​Icterus ​Mild OK
​Other EDTA or serum or specimen NOT protected from light
Useful For
Evaluation of persons who present the signs of ariboflavinosis​
Reference Range Information
Performing Location Reference Range
​Mayo Medical Laboratories ​1-19 mcg/mL
Interpretation
Low concentrations in the blood plasma are indicative of nutritional deficiency. Concentrations <1 mcg/L are considered significantly diminished. Marginally low levels probably represent nutritional deficiency that should be corrected. ​
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Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories ​Monday, Wednesday, Friday 2 days ​
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)​
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​84252
For most current information refer to the Marshfield Laboratory online reference manual.