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25006 Oligoclonal Banding, CSF and Serum

Oligoclonal Banding, CSF and Serum
Test Code: OLIGO
Synonyms/Keywords
​​Oligoclonal Banding in CSF​
Useful For
This test is useful to help determine if increased CSF proteins are due to increased permeability of the blood - CSF barrier, or due to synthesis of immunoglobulins (primarily IgG) within the Central Nervous System.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​
CSF
and
Serum required
Spinal Fluid collection tube for CSF and
 
Red Top Tube (RTT) for Serum

Plastic tube for CSF​

 

Serum Separator Tube (SST) for Serum

1.0 mL CSF

 

1.0 mL Serum​

0.5 mL CSF

 

0.5 mL Serum​

0.5 mL CSF

 

0.5 mL Serum​

Collection Processing Instructions
Serum sample must be drawn within 24 hours of spinal fluid collection.
Separate serum/plasma from cells within 2 hours of collection.
If serum is not collected within 24 hours of CSF collection, please call Customer Service to discuss the case with a Clinical Chemist.
Specimen Stability Information
Specimen Type Temperature Time
CSF/Serum​ ​ Refrigerate​ 7 days​
Frozen 1 month​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Thursday​ 6-8 hours​ Isoelectrofocusing with IgG detection/Sebia Hydrasys​
Test Information
This procedure involves isoelectrofocusing on an agarose gel to fractionate the proteins in the CSF and serum.  This is followed by immunofixation with anti-IgG antiserum.  The use of enzyme-labeled (peroxidase) anti-IgG antiserum permits detection of IgG oligoclonal banding at increased specificity and sensitivity.  The serum is needed to determine if oligoclonal bands are produced only in the Central Nervous System.​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
83916​
Synonyms/Keywords
​​Oligoclonal Banding in CSF​
Ordering Applications
Ordering Application Description
​Centricity ​Oligoclonal Banding
​Cerner ​Oligoclonal Bands CSF and Serum
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​
CSF
and
Serum required
Spinal Fluid collection tube for CSF and
 
Red Top Tube (RTT) for Serum

Plastic tube for CSF​

 

Serum Separator Tube (SST) for Serum

1.0 mL CSF

 

1.0 mL Serum​

0.5 mL CSF

 

0.5 mL Serum​

0.5 mL CSF

 

0.5 mL Serum​

Collection Processing
Serum sample must be drawn within 24 hours of spinal fluid collection.
Separate serum/plasma from cells within 2 hours of collection.
If serum is not collected within 24 hours of CSF collection, please call Customer Service to discuss the case with a Clinical Chemist.
Specimen Stability Information
Specimen Type Temperature Time
CSF/Serum​ ​ Refrigerate​ 7 days​
Frozen 1 month​
Useful For
This test is useful to help determine if increased CSF proteins are due to increased permeability of the blood - CSF barrier, or due to synthesis of immunoglobulins (primarily IgG) within the Central Nervous System.​
Reference Range Information
Performing Location Reference Range
Marshfield​ Negative​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Thursday​ 6-8 hours​ Isoelectrofocusing with IgG detection/Sebia Hydrasys​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
83916​
For most current information refer to the Marshfield Laboratory online reference manual.