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25047 Legionella species, PCR (LEGRP)

Legionella species, PCR (LEGRP)
Test Code: LEGPSO
Synonyms/Keywords
​​​Fluoribacter bozemanae
Fluoribacter dumoffii
Legionella
Legionella sp
Legionella spp
Legionella bozemanae
Legionella dumoffii
Legionella gormanii
Legionella hackeliae
Legionella jordanis
Legionella longbeachae
Legionella micdadei
Legionella oakridgensis
Legionella pneumophila
Legionella wadsworthii
Legionellosis
Legionnaires disease
Useful For

Sensitive and rapid diagnosis of pneumonia caused by Legionella species

The assay is not recommended as a test of cure because bacteria nucleic acids may persist after successful treatment. 

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Sputum, tracheal secretions/aspirates, transtracheal aspirate, bronchial washing/aspirate, bronchoalveolar lavage, lung fluid or pleural fluid Sterile container​ ​ ​1 mL ​0.5 mL ​
​Lung Tissue or Lung Biopsy​Sterile container​​Entire Collection​5 mm(3)​
Specimen Stability Information
Specimen Type Temperature Time
​Respiratory specimen ​ ​Refrigerated (preferred) ​7 days
​Frozen​7 days
​Lung tissue ​ ​Refrigerated (preferred) ​7 days
​Frozen ​7 days
Rejection Criteria
Tissue/OtherTissue in formalin, formaldehyde, or acetone; formalin-fixed paraffin-embedded (FFPE) block
Interference

​This assay does not differentiate between the Legionella species. False-positive results are theoretically possible if patient specimens are contaminated with Legionella DNA, which may occur since Legionella species are environmental organisms present in aquatic environments. The following uncommonly encountered species of Legionella are not detected by this assay: Legionella anisa, L feeleii, L maceachernii, L parisiensis, and L sainthelensi.

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories Monday through Sunday​ 3 day​s
Rapid Polymerase Chain Reaction (PCR)
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)​
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​Not applicable
Interpretation

A positive PCR result for the presence of a specific sequence found within the Legionella 5S rRNA gene indicates the presence of a Legionella species DNA, which may be due to Legionella infection or environmental/water Legionella DNA in the specimen.

A negative PCR result indicates the absence of detectable Legionella DNA in the specimen, but does not rule-out legionellosis as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of Legionella species in quantities less than the limit of detection of the assay.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87801 ​ ​ ​ ​
Synonyms/Keywords
​​​Fluoribacter bozemanae
Fluoribacter dumoffii
Legionella
Legionella sp
Legionella spp
Legionella bozemanae
Legionella dumoffii
Legionella gormanii
Legionella hackeliae
Legionella jordanis
Legionella longbeachae
Legionella micdadei
Legionella oakridgensis
Legionella pneumophila
Legionella wadsworthii
Legionellosis
Legionnaires disease
Ordering Applications
Ordering Application Description
​Centricity ​Legionella Species, PCR (LEGRP)
​Cerner ​Legionella Sps, PCR (89564)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
Sputum, tracheal secretions/aspirates, transtracheal aspirate, bronchial washing/aspirate, bronchoalveolar lavage, lung fluid or pleural fluid Sterile container​ ​ ​1 mL ​0.5 mL ​
​Lung Tissue or Lung Biopsy​Sterile container​​Entire Collection​5 mm(3)​
Specimen Stability Information
Specimen Type Temperature Time
​Respiratory specimen ​ ​Refrigerated (preferred) ​7 days
​Frozen​7 days
​Lung tissue ​ ​Refrigerated (preferred) ​7 days
​Frozen ​7 days
Rejection Criteria
Tissue/OtherTissue in formalin, formaldehyde, or acetone; formalin-fixed paraffin-embedded (FFPE) block
Interference

​This assay does not differentiate between the Legionella species. False-positive results are theoretically possible if patient specimens are contaminated with Legionella DNA, which may occur since Legionella species are environmental organisms present in aquatic environments. The following uncommonly encountered species of Legionella are not detected by this assay: Legionella anisa, L feeleii, L maceachernii, L parisiensis, and L sainthelensi.

Useful For

Sensitive and rapid diagnosis of pneumonia caused by Legionella species

The assay is not recommended as a test of cure because bacteria nucleic acids may persist after successful treatment. 

Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories ​Not applicable
Interpretation

A positive PCR result for the presence of a specific sequence found within the Legionella 5S rRNA gene indicates the presence of a Legionella species DNA, which may be due to Legionella infection or environmental/water Legionella DNA in the specimen.

A negative PCR result indicates the absence of detectable Legionella DNA in the specimen, but does not rule-out legionellosis as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of Legionella species in quantities less than the limit of detection of the assay.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories Monday through Sunday​ 3 day​s
Rapid Polymerase Chain Reaction (PCR)
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)​
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87801 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.